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Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Stålbrand, Henrik LU ; Lundqvist, Jon LU ; Hägglund, Per LU ; Anderson, Lars LU ; Tjerneld, Folke LU ; Jacobs, A; Teleman, A; Dahlman, O; Palm, Magnus LU and Zacchi, Guido LU , et al. (2004) In Hemicelluloses. Science and Technology (ACS Symposium Series) 864. p.66-78
Abstract
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard... (More)
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase. (Less)
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DEGRADING ENZYMES, MICROWAVE IRRADIATION, BETA-MANNANASE, HEMICELLULOSES, PRETREATMENT, CHROMATOGRAPHY, LIGNOCELLULOSE, GLUCOMANNAN, POSITIONS, PINUS-SILVESTRIS L
in
Hemicelluloses. Science and Technology (ACS Symposium Series)
volume
864
pages
66 - 78
publisher
The American Chemical Society
external identifiers
  • wos:000187496800005
  • scopus:38849099768
ISSN
0097-6156
ISBN
978-0-8412-3842-8
0-8412-3842-1
language
English
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yes
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770de003-61c4-4506-8a7e-b20dd47782cc (old id 291446)
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http://www.oup.com/us/catalog/general/subject/Chemistry/IndustrialChemistry/?view=usa&ci=0841238421
date added to LUP
2007-10-26 11:25:20
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2017-06-11 04:29:33
@inbook{770de003-61c4-4506-8a7e-b20dd47782cc,
  abstract     = {Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase.},
  author       = {Stålbrand, Henrik and Lundqvist, Jon and Hägglund, Per and Anderson, Lars and Tjerneld, Folke and Jacobs, A and Teleman, A and Dahlman, O and Palm, Magnus and Zacchi, Guido and Andersson, Alexandra},
  isbn         = {978-0-8412-3842-8},
  issn         = {0097-6156},
  keyword      = {DEGRADING ENZYMES,MICROWAVE IRRADIATION,BETA-MANNANASE,HEMICELLULOSES,PRETREATMENT,CHROMATOGRAPHY,LIGNOCELLULOSE,GLUCOMANNAN,POSITIONS,PINUS-SILVESTRIS L},
  language     = {eng},
  pages        = {66--78},
  publisher    = {The American Chemical Society},
  series       = {Hemicelluloses. Science and Technology (ACS Symposium Series)},
  title        = {Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)},
  volume       = {864},
  year         = {2004},
}