Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)
(2004) 864. p.66-78- Abstract
- Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard... (More)
- Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/291446
- author
- organization
- publishing date
- 2004
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- DEGRADING ENZYMES, MICROWAVE IRRADIATION, BETA-MANNANASE, HEMICELLULOSES, PRETREATMENT, CHROMATOGRAPHY, LIGNOCELLULOSE, GLUCOMANNAN, POSITIONS, PINUS-SILVESTRIS L
- host publication
- Hemicelluloses. Science and Technology (ACS Symposium Series)
- volume
- 864
- pages
- 66 - 78
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000187496800005
- scopus:38849099768
- ISSN
- 0097-6156
- ISBN
- 978-0-8412-3842-8
- 0-8412-3842-1
- language
- English
- LU publication?
- yes
- id
- 770de003-61c4-4506-8a7e-b20dd47782cc (old id 291446)
- alternative location
- http://www.oup.com/us/catalog/general/subject/Chemistry/IndustrialChemistry/?view=usa&ci=0841238421
- date added to LUP
- 2016-04-01 16:12:21
- date last changed
- 2023-10-02 15:28:32
@inbook{770de003-61c4-4506-8a7e-b20dd47782cc, abstract = {{Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase.}}, author = {{Stålbrand, Henrik and Lundqvist, Jon and Hägglund, Per and Anderson, Lars and Tjerneld, Folke and Jacobs, A and Teleman, A and Dahlman, O and Palm, Magnus and Zacchi, Guido and Andersson, Alexandra}}, booktitle = {{Hemicelluloses. Science and Technology (ACS Symposium Series)}}, isbn = {{978-0-8412-3842-8}}, issn = {{0097-6156}}, keywords = {{DEGRADING ENZYMES; MICROWAVE IRRADIATION; BETA-MANNANASE; HEMICELLULOSES; PRETREATMENT; CHROMATOGRAPHY; LIGNOCELLULOSE; GLUCOMANNAN; POSITIONS; PINUS-SILVESTRIS L}}, language = {{eng}}, pages = {{66--78}}, publisher = {{The American Chemical Society (ACS)}}, title = {{Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)}}, url = {{http://www.oup.com/us/catalog/general/subject/Chemistry/IndustrialChemistry/?view=usa&ci=0841238421}}, volume = {{864}}, year = {{2004}}, }