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Comparative cytogenetic and DNA flow cytometric analysis of 242 primary breast carcinomas

Adeyinka, Adewale LU ; Baldetorp, Bo LU ; Mertens, Fredrik LU ; Olsson, Håkan LU ; Johannsson, Oskar LU ; Heim, Sverre LU and Pandis, Nikos (2003) In Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00 147(1). p.62-67
Abstract

The cytogenetic and DNA flow cytometric findings in 242 breast carcinomas were compared. The combined use of both techniques improved the detection of abnormal cell populations from 65% by cytogenetic analysis alone and 59% by DNA flow cytometric analysis alone to 84%. Informative and comparable cytogenetic and flow cytometric data were obtained for 155 tumors. Among these 155 tumors, there was good concordance (64%) between the estimates of genomic changes by the two methods. Most discrepancies were among the DNA-diploid cases, where cytogenetic analysis detected small genomic changes. There were, however, also some exceptions in which large genomic changes detected by one method were missed by the other. Of the specific breast... (More)

The cytogenetic and DNA flow cytometric findings in 242 breast carcinomas were compared. The combined use of both techniques improved the detection of abnormal cell populations from 65% by cytogenetic analysis alone and 59% by DNA flow cytometric analysis alone to 84%. Informative and comparable cytogenetic and flow cytometric data were obtained for 155 tumors. Among these 155 tumors, there was good concordance (64%) between the estimates of genomic changes by the two methods. Most discrepancies were among the DNA-diploid cases, where cytogenetic analysis detected small genomic changes. There were, however, also some exceptions in which large genomic changes detected by one method were missed by the other. Of the specific breast cancer-associated cytogenetic aberrations subjected to separate correlation analysis, polysomy for chromosome 20 was significantly associated with a high S-phase fraction, whereas loss of the long arm of chromosome 16 and/or the presence of a der(1;16) were significantly associated with a low S-phase fraction. Our data show that cytogenetic and DNA flow cytometric analyses of breast carcinomas give largely comparable results, and that combining data from both methods significantly improves the information obtained by either technique used alone on the genetic abnormalities in these tumors.

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keywords
Breast Neoplasms, Carcinoma, Chromosome Aberrations, Chromosome Mapping, Chromosomes, Human, Pair 16, Chromosomes, Human, Pair 20, Cytogenetic Analysis, Diploidy, Female, Flow Cytometry, Humans, Karyotyping, Ploidies, S Phase, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00
volume
147
issue
1
pages
6 pages
publisher
Elsevier
external identifiers
  • pmid:14580772
  • wos:000186363100009
  • scopus:0142217431
ISSN
0165-4608
DOI
10.1016/S0165-4608(03)00190-0
language
English
LU publication?
yes
id
c519c802-dec4-4eaf-9813-17f29a7eb6c7 (old id 296557)
date added to LUP
2007-08-21 12:29:47
date last changed
2018-01-07 08:42:16
@article{c519c802-dec4-4eaf-9813-17f29a7eb6c7,
  abstract     = {<p>The cytogenetic and DNA flow cytometric findings in 242 breast carcinomas were compared. The combined use of both techniques improved the detection of abnormal cell populations from 65% by cytogenetic analysis alone and 59% by DNA flow cytometric analysis alone to 84%. Informative and comparable cytogenetic and flow cytometric data were obtained for 155 tumors. Among these 155 tumors, there was good concordance (64%) between the estimates of genomic changes by the two methods. Most discrepancies were among the DNA-diploid cases, where cytogenetic analysis detected small genomic changes. There were, however, also some exceptions in which large genomic changes detected by one method were missed by the other. Of the specific breast cancer-associated cytogenetic aberrations subjected to separate correlation analysis, polysomy for chromosome 20 was significantly associated with a high S-phase fraction, whereas loss of the long arm of chromosome 16 and/or the presence of a der(1;16) were significantly associated with a low S-phase fraction. Our data show that cytogenetic and DNA flow cytometric analyses of breast carcinomas give largely comparable results, and that combining data from both methods significantly improves the information obtained by either technique used alone on the genetic abnormalities in these tumors.</p>},
  author       = {Adeyinka, Adewale and Baldetorp, Bo and Mertens, Fredrik and Olsson, Håkan and Johannsson, Oskar and Heim, Sverre and Pandis, Nikos},
  issn         = {0165-4608},
  keyword      = {Breast Neoplasms,Carcinoma,Chromosome Aberrations,Chromosome Mapping,Chromosomes, Human, Pair 16,Chromosomes, Human, Pair 20,Cytogenetic Analysis,Diploidy,Female,Flow Cytometry,Humans,Karyotyping,Ploidies,S Phase,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {62--67},
  publisher    = {Elsevier},
  series       = {Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00},
  title        = {Comparative cytogenetic and DNA flow cytometric analysis of 242 primary breast carcinomas},
  url          = {http://dx.doi.org/10.1016/S0165-4608(03)00190-0},
  volume       = {147},
  year         = {2003},
}