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Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part 1. A theoretical approach.

Håkansson, Andreas LU ; Magnusson, Emma LU ; Bergenståhl, Björn LU and Nilsson, Lars LU (2012) In Journal of chromatography. A 1253. p.120-126
Abstract
Direct determination of hydrodynamic radius from retention time is an advantage of the field-flow fractionation techniques. However, this is not always completely straight forward since non-idealities exist and assumptions have been made in deriving the retention equations. In this study we investigate the effect on accuracy from two factors: (1) level of sophistication of the equations used to determine channel height from a calibration experiment and (2) the influence of secondary relaxation on the accuracy of hydrodynamic radius determination. A new improved technique for estimating the channel height from calibration experiments is suggested. It is concluded that severe systematic error can arise if the most common channel height... (More)
Direct determination of hydrodynamic radius from retention time is an advantage of the field-flow fractionation techniques. However, this is not always completely straight forward since non-idealities exist and assumptions have been made in deriving the retention equations. In this study we investigate the effect on accuracy from two factors: (1) level of sophistication of the equations used to determine channel height from a calibration experiment and (2) the influence of secondary relaxation on the accuracy of hydrodynamic radius determination. A new improved technique for estimating the channel height from calibration experiments is suggested. It is concluded that severe systematic error can arise if the most common channel height equations are used and an alternative more rigorous approach is described. For secondary relaxation it is concluded that this effect increases with the cross-flow decay rate. The secondary relaxation effect is quantified for different conditions. This is part one of two. In the second part the determination of hydrodynamic radius are evaluated experimentally under similar conditions. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
radius, AsFlFFF, Programmed cross-flow, Hydrodynamic, Secondary relaxation
in
Journal of chromatography. A
volume
1253
pages
120 - 126
publisher
Elsevier
external identifiers
  • wos:000307793700014
  • pmid:22835686
  • scopus:84864566541
ISSN
1873-3778
DOI
10.1016/j.chroma.2012.07.029
language
English
LU publication?
yes
id
6215fbbe-a4e7-4133-891b-e32021d4d2f5 (old id 2966526)
date added to LUP
2012-09-03 12:41:02
date last changed
2017-08-20 04:13:30
@article{6215fbbe-a4e7-4133-891b-e32021d4d2f5,
  abstract     = {Direct determination of hydrodynamic radius from retention time is an advantage of the field-flow fractionation techniques. However, this is not always completely straight forward since non-idealities exist and assumptions have been made in deriving the retention equations. In this study we investigate the effect on accuracy from two factors: (1) level of sophistication of the equations used to determine channel height from a calibration experiment and (2) the influence of secondary relaxation on the accuracy of hydrodynamic radius determination. A new improved technique for estimating the channel height from calibration experiments is suggested. It is concluded that severe systematic error can arise if the most common channel height equations are used and an alternative more rigorous approach is described. For secondary relaxation it is concluded that this effect increases with the cross-flow decay rate. The secondary relaxation effect is quantified for different conditions. This is part one of two. In the second part the determination of hydrodynamic radius are evaluated experimentally under similar conditions.},
  author       = {Håkansson, Andreas and Magnusson, Emma and Bergenståhl, Björn and Nilsson, Lars},
  issn         = {1873-3778},
  keyword      = {radius,AsFlFFF,Programmed cross-flow,Hydrodynamic,Secondary relaxation},
  language     = {eng},
  pages        = {120--126},
  publisher    = {Elsevier},
  series       = {Journal of chromatography. A},
  title        = {Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part 1. A theoretical approach.},
  url          = {http://dx.doi.org/10.1016/j.chroma.2012.07.029},
  volume       = {1253},
  year         = {2012},
}