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Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part II. Experimental evaluation.

Magnusson, Emma LU ; Håkansson, Andreas LU ; Janiak, John LU ; Bergenståhl, Björn LU and Nilsson, Lars LU (2012) In Journal of chromatography. A 1253. p.127-133
Abstract
In this study we investigate the effect of programmed cross-flows on the error in the hydrodynamic radii (r(h)) determination with asymmetrical flow field-flow fractionation (AsFlFFF). Three different standard polystyrene particles (nominal radii of 30 and 40 and 50nm) are fractionated with exponentially and linearly decaying cross-flows with different decay rates. Hydrodynamic radii are calculated according to retention theory including steric effects. Rapid decay is expected to give rise to systematic deviations in r(h) determination. The error in r(h) was found to be small when decay rates with half-lives longer than 6min were used, whereas steeper decays could give rise to errors as high as 16% of the particle size. The error is often... (More)
In this study we investigate the effect of programmed cross-flows on the error in the hydrodynamic radii (r(h)) determination with asymmetrical flow field-flow fractionation (AsFlFFF). Three different standard polystyrene particles (nominal radii of 30 and 40 and 50nm) are fractionated with exponentially and linearly decaying cross-flows with different decay rates. Hydrodynamic radii are calculated according to retention theory including steric effects. Rapid decay is expected to give rise to systematic deviations in r(h) determination. The error in r(h) was found to be small when decay rates with half-lives longer than 6min were used, whereas steeper decays could give rise to errors as high as 16% of the particle size. The error is often explained in terms of secondary relaxation. However, comparisons show that experimental errors are significantly larger than what would be expected due to secondary relaxation, suggesting that other factors also have to be considered in order to fully understand deviations for rapidly decaying cross-flow. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of chromatography. A
volume
1253
pages
127 - 133
publisher
Elsevier
external identifiers
  • wos:000307793700015
  • pmid:22818771
  • scopus:84864570841
ISSN
1873-3778
DOI
10.1016/j.chroma.2012.07.005
language
English
LU publication?
yes
id
81071597-96d3-457c-a366-fd8aa1e46d9f (old id 2966839)
date added to LUP
2012-09-03 12:37:25
date last changed
2017-01-15 03:53:56
@article{81071597-96d3-457c-a366-fd8aa1e46d9f,
  abstract     = {In this study we investigate the effect of programmed cross-flows on the error in the hydrodynamic radii (r(h)) determination with asymmetrical flow field-flow fractionation (AsFlFFF). Three different standard polystyrene particles (nominal radii of 30 and 40 and 50nm) are fractionated with exponentially and linearly decaying cross-flows with different decay rates. Hydrodynamic radii are calculated according to retention theory including steric effects. Rapid decay is expected to give rise to systematic deviations in r(h) determination. The error in r(h) was found to be small when decay rates with half-lives longer than 6min were used, whereas steeper decays could give rise to errors as high as 16% of the particle size. The error is often explained in terms of secondary relaxation. However, comparisons show that experimental errors are significantly larger than what would be expected due to secondary relaxation, suggesting that other factors also have to be considered in order to fully understand deviations for rapidly decaying cross-flow.},
  author       = {Magnusson, Emma and Håkansson, Andreas and Janiak, John and Bergenståhl, Björn and Nilsson, Lars},
  issn         = {1873-3778},
  language     = {eng},
  pages        = {127--133},
  publisher    = {Elsevier},
  series       = {Journal of chromatography. A},
  title        = {Hydrodynamic radius determination with asymmetrical flow field-flow fractionation using decaying cross-flows. Part II. Experimental evaluation.},
  url          = {http://dx.doi.org/10.1016/j.chroma.2012.07.005},
  volume       = {1253},
  year         = {2012},
}