Ultrasound standing wave spatial patterning of human umbilical vein endothelial cells for 3D micro-vascular networks formation
(2023) In Biofabrication 16(1). p.015009-015009- Abstract
- Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving several different factors such as time consuming, cells survival, micro-diameter vasculature and strict alignment. Here, we propose a technique combining multi-material extrusion and ultrasound standing wave forces to create a network structure of human umbilical vein endothelial cells within a mixture of calcium alginate and decellularized extracellular matrix. The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell–cell adhesion, angiogenesis process, and... (More)
- Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving several different factors such as time consuming, cells survival, micro-diameter vasculature and strict alignment. Here, we propose a technique combining multi-material extrusion and ultrasound standing wave forces to create a network structure of human umbilical vein endothelial cells within a mixture of calcium alginate and decellularized extracellular matrix. The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell–cell adhesion, angiogenesis process, and perfusion tests with microparticles, FITC-dextran, and whole mouse blood. Moreover, animal experiments exhibited the implantability including that the pre-existing blood vessels of the host sprout towards the preformed vessels of the scaffold over time and the microvessels inside the implanted scaffold matured from empty tubular structures to functional blood-carrying microvessels in two weeks. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2985125c-4fa1-47e4-9792-4bb6d8b1d6c4
- author
- Le, Huong Thi ; Phan, Huu Lam ; Lenshof, Andreas LU ; Choi, Cholong ; Cha, Chaenyung ; Laurell, Thomas LU and Koo, Kyo-in LU
- organization
-
- Department of Biomedical Engineering
- Acoustofluidics group (research group)
- NanoLund: Centre for Nanoscience
- Electrical Engineering (M.Sc.Eng.)
- LUCC: Lund University Cancer Centre
- LTH Profile Area: Engineering Health
- LTH Profile Area: Nanoscience and Semiconductor Technology
- LU Profile Area: Light and Materials
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biofabrication
- volume
- 16
- issue
- 1
- pages
- 1 pages
- publisher
- IOP Publishing
- external identifiers
-
- scopus:85176499835
- pmid:37844581
- ISSN
- 1758-5082
- DOI
- 10.1088/1758-5090/ad03be
- language
- English
- LU publication?
- yes
- id
- 2985125c-4fa1-47e4-9792-4bb6d8b1d6c4
- date added to LUP
- 2023-11-24 13:24:55
- date last changed
- 2023-11-29 03:00:02
@article{2985125c-4fa1-47e4-9792-4bb6d8b1d6c4, abstract = {{Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving several different factors such as time consuming, cells survival, micro-diameter vasculature and strict alignment. Here, we propose a technique combining multi-material extrusion and ultrasound standing wave forces to create a network structure of human umbilical vein endothelial cells within a mixture of calcium alginate and decellularized extracellular matrix. The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell–cell adhesion, angiogenesis process, and perfusion tests with microparticles, FITC-dextran, and whole mouse blood. Moreover, animal experiments exhibited the implantability including that the pre-existing blood vessels of the host sprout towards the preformed vessels of the scaffold over time and the microvessels inside the implanted scaffold matured from empty tubular structures to functional blood-carrying microvessels in two weeks.}}, author = {{Le, Huong Thi and Phan, Huu Lam and Lenshof, Andreas and Choi, Cholong and Cha, Chaenyung and Laurell, Thomas and Koo, Kyo-in}}, issn = {{1758-5082}}, language = {{eng}}, number = {{1}}, pages = {{015009--015009}}, publisher = {{IOP Publishing}}, series = {{Biofabrication}}, title = {{Ultrasound standing wave spatial patterning of human umbilical vein endothelial cells for 3D micro-vascular networks formation}}, url = {{http://dx.doi.org/10.1088/1758-5090/ad03be}}, doi = {{10.1088/1758-5090/ad03be}}, volume = {{16}}, year = {{2023}}, }