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Ultrasound standing wave spatial patterning of human umbilical vein endothelial cells for 3D micro-vascular networks formation

Le, Huong Thi ; Phan, Huu Lam ; Lenshof, Andreas LU ; Choi, Cholong ; Cha, Chaenyung ; Laurell, Thomas LU and Koo, Kyo-in LU orcid (2023) In Biofabrication 16(1). p.015009-015009
Abstract
Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving several different factors such as time consuming, cells survival, micro-diameter vasculature and strict alignment. Here, we propose a technique combining multi-material extrusion and ultrasound standing wave forces to create a network structure of human umbilical vein endothelial cells within a mixture of calcium alginate and decellularized extracellular matrix. The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell–cell adhesion, angiogenesis process, and... (More)
Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving several different factors such as time consuming, cells survival, micro-diameter vasculature and strict alignment. Here, we propose a technique combining multi-material extrusion and ultrasound standing wave forces to create a network structure of human umbilical vein endothelial cells within a mixture of calcium alginate and decellularized extracellular matrix. The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell–cell adhesion, angiogenesis process, and perfusion tests with microparticles, FITC-dextran, and whole mouse blood. Moreover, animal experiments exhibited the implantability including that the pre-existing blood vessels of the host sprout towards the preformed vessels of the scaffold over time and the microvessels inside the implanted scaffold matured from empty tubular structures to functional blood-carrying microvessels in two weeks. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biofabrication
volume
16
issue
1
pages
1 pages
publisher
IOP Publishing
external identifiers
  • scopus:85176499835
  • pmid:37844581
ISSN
1758-5082
DOI
10.1088/1758-5090/ad03be
language
English
LU publication?
yes
id
2985125c-4fa1-47e4-9792-4bb6d8b1d6c4
date added to LUP
2023-11-24 13:24:55
date last changed
2023-11-29 03:00:02
@article{2985125c-4fa1-47e4-9792-4bb6d8b1d6c4,
  abstract     = {{Generating functional and perfusable micro-vascular networks is an important goal for the fabrication of large and three-dimensional tissues. Up to now, the fabrication of micro-vascular networks is a complicated multitask involving several different factors such as time consuming, cells survival, micro-diameter vasculature and strict alignment. Here, we propose a technique combining multi-material extrusion and ultrasound standing wave forces to create a network structure of human umbilical vein endothelial cells within a mixture of calcium alginate and decellularized extracellular matrix. The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell–cell adhesion, angiogenesis process, and perfusion tests with microparticles, FITC-dextran, and whole mouse blood. Moreover, animal experiments exhibited the implantability including that the pre-existing blood vessels of the host sprout towards the preformed vessels of the scaffold over time and the microvessels inside the implanted scaffold matured from empty tubular structures to functional blood-carrying microvessels in two weeks.}},
  author       = {{Le, Huong Thi and Phan, Huu Lam and Lenshof, Andreas and Choi, Cholong and Cha, Chaenyung and Laurell, Thomas and Koo, Kyo-in}},
  issn         = {{1758-5082}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{015009--015009}},
  publisher    = {{IOP Publishing}},
  series       = {{Biofabrication}},
  title        = {{Ultrasound standing wave spatial patterning of human umbilical vein endothelial cells for 3D micro-vascular networks formation}},
  url          = {{http://dx.doi.org/10.1088/1758-5090/ad03be}},
  doi          = {{10.1088/1758-5090/ad03be}},
  volume       = {{16}},
  year         = {{2023}},
}