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Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs

Hambraeus, Gustav LU ; von Wachenfeldt, Claes LU and Hederstedt, Lars LU (2003) In Molecular Genetics and Genomics2001-01-01+01:00 269(5). p.706-714
Abstract
We have used DNA microarrays to survey rates of mRNA decay on a genomic scale in early stationary-phase cultures of Bacillus subtilis. The decay rates for mRNAs corresponding to about 1500 genes could be estimated. About 80% of these mRNAs had a half-life of less than 7 min. More than 30 mRNAs, including both mono- and polycistronic transcripts, were found to be extremely stable, i.e. to have a half-life of greater than or equal to15 min. Only two such transcripts were known previously in B. subtilis. The results provide the first overview of mRNA decay rates in a gram-positive bacterium and help to identify polycistronic operons. We could find no obvious correlation between the stability of an mRNA and the function of the encoded protein.... (More)
We have used DNA microarrays to survey rates of mRNA decay on a genomic scale in early stationary-phase cultures of Bacillus subtilis. The decay rates for mRNAs corresponding to about 1500 genes could be estimated. About 80% of these mRNAs had a half-life of less than 7 min. More than 30 mRNAs, including both mono- and polycistronic transcripts, were found to be extremely stable, i.e. to have a half-life of greater than or equal to15 min. Only two such transcripts were known previously in B. subtilis. The results provide the first overview of mRNA decay rates in a gram-positive bacterium and help to identify polycistronic operons. We could find no obvious correlation between the stability of an mRNA and the function of the encoded protein. We have also not found any general features in the 5' regions of mRNAs that distinguish stable from unstable transcripts. The identified set of extremely stable mRNAs may be useful in the construction of stable recombinant genes for the overproduction of biomolecules in Bacillus species. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gram-positive bacteria, mRNA stability, decay rates, microarrays, global analysis
in
Molecular Genetics and Genomics2001-01-01+01:00
volume
269
issue
5
pages
706 - 714
publisher
Springer
external identifiers
  • pmid:12884008
  • wos:000185553600014
  • scopus:0042330135
ISSN
1617-4615
DOI
10.1007/s00438-003-0883-6
language
English
LU publication?
yes
id
5eb8608d-f549-4b45-9720-f1e5b223b02b (old id 299705)
date added to LUP
2007-09-03 14:36:29
date last changed
2018-10-14 04:09:53
@article{5eb8608d-f549-4b45-9720-f1e5b223b02b,
  abstract     = {We have used DNA microarrays to survey rates of mRNA decay on a genomic scale in early stationary-phase cultures of Bacillus subtilis. The decay rates for mRNAs corresponding to about 1500 genes could be estimated. About 80% of these mRNAs had a half-life of less than 7 min. More than 30 mRNAs, including both mono- and polycistronic transcripts, were found to be extremely stable, i.e. to have a half-life of greater than or equal to15 min. Only two such transcripts were known previously in B. subtilis. The results provide the first overview of mRNA decay rates in a gram-positive bacterium and help to identify polycistronic operons. We could find no obvious correlation between the stability of an mRNA and the function of the encoded protein. We have also not found any general features in the 5' regions of mRNAs that distinguish stable from unstable transcripts. The identified set of extremely stable mRNAs may be useful in the construction of stable recombinant genes for the overproduction of biomolecules in Bacillus species.},
  author       = {Hambraeus, Gustav and von Wachenfeldt, Claes and Hederstedt, Lars},
  issn         = {1617-4615},
  keyword      = {gram-positive bacteria,mRNA stability,decay rates,microarrays,global analysis},
  language     = {eng},
  number       = {5},
  pages        = {706--714},
  publisher    = {Springer},
  series       = {Molecular Genetics and Genomics2001-01-01+01:00},
  title        = {Genome-wide survey of mRNA half-lives in <em>Bacillus subtilis</em> identifies extremely stable mRNAs},
  url          = {http://dx.doi.org/10.1007/s00438-003-0883-6},
  volume       = {269},
  year         = {2003},
}