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Interactions between cultured bovine arterial endothelial and smooth muscle cells : effects of injury on the release of growth stimulating and growth inhibiting substances

Xu, C B LU ; Pessah-Rasmussen, H LU and Stavenow, L. (1991) In Pharmacology and Toxicology 69(3). p.195-200
Abstract

Dimethylsulfoxide-soluble particles (DSP) from cigarette smoke and ultraviolet light caused a low degree (cell death less than 30%) and high degree (cell death 60-90%) injury to bovine arterial endothelial cells and smooth muscle cells in culture. Conditioned medium from low degree injured endothelial cells and smooth muscle cells generally inhibited DNA synthesis in new smooth muscle cells or endothelial cells while high degree injury increased DNA synthesis in new cells. Specifically, the growth stimulating activity from endothelial cells was decreased after low degree injury but increased after high degree. UV light released more growth stimulating substances from smooth muscle cells after both low and high degree injury. The release... (More)

Dimethylsulfoxide-soluble particles (DSP) from cigarette smoke and ultraviolet light caused a low degree (cell death less than 30%) and high degree (cell death 60-90%) injury to bovine arterial endothelial cells and smooth muscle cells in culture. Conditioned medium from low degree injured endothelial cells and smooth muscle cells generally inhibited DNA synthesis in new smooth muscle cells or endothelial cells while high degree injury increased DNA synthesis in new cells. Specifically, the growth stimulating activity from endothelial cells was decreased after low degree injury but increased after high degree. UV light released more growth stimulating substances from smooth muscle cells after both low and high degree injury. The release of growth inhibiting substances was dependent on both cell kind and degree of injury. In co-culture low and high degree DSP injury to endothelial cells inhibited smooth muscle cell proliferation, which was in contrast to the effect of conditioned medium from high degree injured endothelial cells. Conditioned medium from endothelial cells treated with LDL and glucose inhibited DNA synthesis in smooth muscle cells. It is concluded that injury to endothelial cells or smooth muscle cells will modify the release of growth inhibiting and growth stimulating activity and that this release will depend on cell kind as well as degree and kind of the injurious stimulus.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Cattle, Cell Count, Cell Division, Cell Survival, Cells, Cultured, DNA, Endothelium, Vascular, Glucose, Growth Inhibitors, Growth Substances, Lipoproteins, LDL, Muscle, Smooth, Vascular, Smoke, Ultraviolet Rays, Journal Article, Research Support, Non-U.S. Gov't
in
Pharmacology and Toxicology
volume
69
issue
3
pages
195 - 200
publisher
Wiley-Blackwell
external identifiers
  • scopus:0025780925
ISSN
0901-9928
DOI
10.1111/j.1600-0773.1991.tb01296.x
language
English
LU publication?
yes
id
2a7cae25-089d-4dbf-ba69-6eb060551da4
date added to LUP
2017-08-11 15:57:54
date last changed
2017-11-06 10:46:42
@article{2a7cae25-089d-4dbf-ba69-6eb060551da4,
  abstract     = {<p>Dimethylsulfoxide-soluble particles (DSP) from cigarette smoke and ultraviolet light caused a low degree (cell death less than 30%) and high degree (cell death 60-90%) injury to bovine arterial endothelial cells and smooth muscle cells in culture. Conditioned medium from low degree injured endothelial cells and smooth muscle cells generally inhibited DNA synthesis in new smooth muscle cells or endothelial cells while high degree injury increased DNA synthesis in new cells. Specifically, the growth stimulating activity from endothelial cells was decreased after low degree injury but increased after high degree. UV light released more growth stimulating substances from smooth muscle cells after both low and high degree injury. The release of growth inhibiting substances was dependent on both cell kind and degree of injury. In co-culture low and high degree DSP injury to endothelial cells inhibited smooth muscle cell proliferation, which was in contrast to the effect of conditioned medium from high degree injured endothelial cells. Conditioned medium from endothelial cells treated with LDL and glucose inhibited DNA synthesis in smooth muscle cells. It is concluded that injury to endothelial cells or smooth muscle cells will modify the release of growth inhibiting and growth stimulating activity and that this release will depend on cell kind as well as degree and kind of the injurious stimulus.</p>},
  author       = {Xu, C B and Pessah-Rasmussen, H and Stavenow, L.},
  issn         = {0901-9928},
  keyword      = {Animals,Cattle,Cell Count,Cell Division,Cell Survival,Cells, Cultured,DNA,Endothelium, Vascular,Glucose,Growth Inhibitors,Growth Substances,Lipoproteins, LDL,Muscle, Smooth, Vascular,Smoke,Ultraviolet Rays,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {195--200},
  publisher    = {Wiley-Blackwell},
  series       = {Pharmacology and Toxicology},
  title        = {Interactions between cultured bovine arterial endothelial and smooth muscle cells : effects of injury on the release of growth stimulating and growth inhibiting substances},
  url          = {http://dx.doi.org/10.1111/j.1600-0773.1991.tb01296.x},
  volume       = {69},
  year         = {1991},
}