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Identification of stage-specific surface markers in early B cell development provides novel tools for identification of progenitor populations

Jensen, Christina T. LU ; Lang, Stefan LU ; Somasundaram, Rajesh; Soneji, Shamit LU and Sigvardsson, Mikael LU (2016) In Journal of Immunology 197(5). p.1937-1944
Abstract

Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage-and stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19+ B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM2 stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that... (More)

Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage-and stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19+ B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM2 stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that selectively marked defined stages of B cell development. Among the stage-specific markers were the cell adhesion proteins CD49E, CD11A, and CD54 that are highly expressed selectively on the most immature progenitors. This work identifies a set of novel stage-specific surface markers that can be used as a complement to the classical staining protocols to explore B lymphocyte development.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
197
issue
5
pages
8 pages
publisher
American Association of Immunologists
external identifiers
  • scopus:84983786111
  • wos:000385002300041
ISSN
0022-1767
DOI
10.4049/jimmunol.1600297
language
English
LU publication?
yes
id
2c9c03e2-0f90-4f6a-937e-ca49eb9bb75e
date added to LUP
2016-12-06 11:34:59
date last changed
2017-04-18 14:06:58
@article{2c9c03e2-0f90-4f6a-937e-ca49eb9bb75e,
  abstract     = {<p>Whereas the characterization of B lymphoid progenitors has been facilitated by the identification of lineage-and stage-specific surface markers, the continued identification of differentially expressed proteins increases our capacity to explore normal and malignant B cell development. To identify novel surface markers with stage-specific expression patterns, we explored the reactivity of CD19+ B cell progenitor cells to Abs targeted to 176 surface proteins. Markers with stage-specific expression were identified using a transgenic reporter gene system subdividing the B cell progenitors into four surface IgM2 stages. This approach affirmed the utility of known stage-specific markers, as well as identifying additional proteins that selectively marked defined stages of B cell development. Among the stage-specific markers were the cell adhesion proteins CD49E, CD11A, and CD54 that are highly expressed selectively on the most immature progenitors. This work identifies a set of novel stage-specific surface markers that can be used as a complement to the classical staining protocols to explore B lymphocyte development.</p>},
  author       = {Jensen, Christina T. and Lang, Stefan and Somasundaram, Rajesh and Soneji, Shamit and Sigvardsson, Mikael},
  issn         = {0022-1767},
  language     = {eng},
  month        = {09},
  number       = {5},
  pages        = {1937--1944},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Identification of stage-specific surface markers in early B cell development provides novel tools for identification of progenitor populations},
  url          = {http://dx.doi.org/10.4049/jimmunol.1600297},
  volume       = {197},
  year         = {2016},
}