Matrilysin Expression Related to Radiation and Microflora Changes in Murine Bowel.
(2011) In The Journal of surgical research 167. p.137-143- Abstract
- BACKGROUND: Matrilysin (MMP-7) elevation after radiotherapy is shown in humans. Matrilysin regulates certain cytokines and the production of bactericidal proteins when the mucosa is exposed to bacterial antigens. We investigate the effect of irradiation on matrilysin and microflora in murine bowel, after modulation with antibiotics. METHODS: Animals were divided into two different groups a radiation group (72 animals) and sham radiation group (36 animals). Animals were divided into smaller groups of six according to radiation dose (19 or 38 Gy or sham). Seven days before radiotherapy ampicillin 500 mg/kg/d was administered intramuscularly, in the antibiotic groups. An exteriorized segment of ileum was subjected to single high dose... (More)
- BACKGROUND: Matrilysin (MMP-7) elevation after radiotherapy is shown in humans. Matrilysin regulates certain cytokines and the production of bactericidal proteins when the mucosa is exposed to bacterial antigens. We investigate the effect of irradiation on matrilysin and microflora in murine bowel, after modulation with antibiotics. METHODS: Animals were divided into two different groups a radiation group (72 animals) and sham radiation group (36 animals). Animals were divided into smaller groups of six according to radiation dose (19 or 38 Gy or sham). Seven days before radiotherapy ampicillin 500 mg/kg/d was administered intramuscularly, in the antibiotic groups. An exteriorized segment of ileum was subjected to single high dose radiation (19 or 38 Gy). Samples were collected 2, 24, and 48 h and analyzed for microflora, MIP-2, TGF-β, and MMP-7. RESULTS: The combination of antibiotics and irradiation leads to an early significant reduction of bacteria, down-regulates MIP-2, up-regulates TGF-β and elevation of MMP-7 to levels achieved by antibiotics or irradiation alone. Lactobacilli were reduced to non-existent levels after antibiotics. CONCLUSIONS: Pretreatment with Ampicillin before irradiation and laparotomy in a murine model leads to Matrilysin over-expression as achieved by radiotherapy alone. Microfloral regulation does not affect MMP-7 stimulation after surgical or radiological trauma. Radiotherapy overrides the effect of antibiotics leading to an up-regulation of MMP-7, TGF-β and MIP-2 expression between 24 h and 48 h. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1831771
- author
- Polistena, Andrea LU ; Johnson, Louis Banka LU ; Röme, Andrada LU ; Wittgren, Lena LU ; Bäck, Sven LU ; Osman, Nadia LU ; Molin, Göran ; Adawi, Diya LU and Jeppsson, Bengt LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of surgical research
- volume
- 167
- pages
- 137 - 143
- publisher
- Elsevier
- external identifiers
-
- wos:000289407800011
- pmid:21324398
- scopus:79954616714
- pmid:21324398
- ISSN
- 1095-8673
- DOI
- 10.1016/j.jss.2010.12.031
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Radiation Physics, Malmö (013243210), Surgery Research Unit (013242220), Emergency medicine/Medicine/Surgery (013240200), Apetite Regulation (013212030)
- id
- 2cb3993f-61be-4cfb-89b4-7f9b120a4e32 (old id 1831771)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21324398?dopt=Abstract
- date added to LUP
- 2016-04-04 09:06:55
- date last changed
- 2022-01-29 08:17:30
@article{2cb3993f-61be-4cfb-89b4-7f9b120a4e32, abstract = {{BACKGROUND: Matrilysin (MMP-7) elevation after radiotherapy is shown in humans. Matrilysin regulates certain cytokines and the production of bactericidal proteins when the mucosa is exposed to bacterial antigens. We investigate the effect of irradiation on matrilysin and microflora in murine bowel, after modulation with antibiotics. METHODS: Animals were divided into two different groups a radiation group (72 animals) and sham radiation group (36 animals). Animals were divided into smaller groups of six according to radiation dose (19 or 38 Gy or sham). Seven days before radiotherapy ampicillin 500 mg/kg/d was administered intramuscularly, in the antibiotic groups. An exteriorized segment of ileum was subjected to single high dose radiation (19 or 38 Gy). Samples were collected 2, 24, and 48 h and analyzed for microflora, MIP-2, TGF-β, and MMP-7. RESULTS: The combination of antibiotics and irradiation leads to an early significant reduction of bacteria, down-regulates MIP-2, up-regulates TGF-β and elevation of MMP-7 to levels achieved by antibiotics or irradiation alone. Lactobacilli were reduced to non-existent levels after antibiotics. CONCLUSIONS: Pretreatment with Ampicillin before irradiation and laparotomy in a murine model leads to Matrilysin over-expression as achieved by radiotherapy alone. Microfloral regulation does not affect MMP-7 stimulation after surgical or radiological trauma. Radiotherapy overrides the effect of antibiotics leading to an up-regulation of MMP-7, TGF-β and MIP-2 expression between 24 h and 48 h.}}, author = {{Polistena, Andrea and Johnson, Louis Banka and Röme, Andrada and Wittgren, Lena and Bäck, Sven and Osman, Nadia and Molin, Göran and Adawi, Diya and Jeppsson, Bengt}}, issn = {{1095-8673}}, language = {{eng}}, pages = {{137--143}}, publisher = {{Elsevier}}, series = {{The Journal of surgical research}}, title = {{Matrilysin Expression Related to Radiation and Microflora Changes in Murine Bowel.}}, url = {{http://dx.doi.org/10.1016/j.jss.2010.12.031}}, doi = {{10.1016/j.jss.2010.12.031}}, volume = {{167}}, year = {{2011}}, }