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Fluorescence Microscopy of Nanochannel-Confined DNA

Kk, Sriram ; Persson, Fredrik ; Fritzsche, Joachim ; Beech, Jason P. LU ; Tegenfeldt, Jonas O. LU orcid and Westerlund, Fredrik (2024) In Methods in Molecular Biology 2694. p.175-202
Abstract

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.

Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DNA, Fluorescence, Nanochannels, Single molecule
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
2694
pages
28 pages
publisher
Humana Press
external identifiers
  • pmid:37824005
  • scopus:85175184695
ISSN
1064-3745
1940-6029
ISBN
978-1-0716-3376-2
978-1-0716-3377-9
DOI
10.1007/978-1-0716-3377-9_9
language
English
LU publication?
yes
additional info
Publisher Copyright: © The Author(s) 2024.
id
2e52ca9a-62e8-4eef-b55f-c44999d5bbfe
date added to LUP
2023-11-12 07:56:04
date last changed
2024-11-06 19:24:19
@inbook{2e52ca9a-62e8-4eef-b55f-c44999d5bbfe,
  abstract     = {{<p>Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.</p>}},
  author       = {{Kk, Sriram and Persson, Fredrik and Fritzsche, Joachim and Beech, Jason P. and Tegenfeldt, Jonas O. and Westerlund, Fredrik}},
  booktitle    = {{Methods in Molecular Biology}},
  isbn         = {{978-1-0716-3376-2}},
  issn         = {{1064-3745}},
  keywords     = {{DNA; Fluorescence; Nanochannels; Single molecule}},
  language     = {{eng}},
  pages        = {{175--202}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Fluorescence Microscopy of Nanochannel-Confined DNA}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-3377-9_9}},
  doi          = {{10.1007/978-1-0716-3377-9_9}},
  volume       = {{2694}},
  year         = {{2024}},
}