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Absence of Parallel Fibre to Purkinje Cell LTD During Eyeblink Conditioning

Johansson, Fredrik LU ; Jirenhed, Dan Anders LU ; Rasmussen, Anders LU ; Zucca, Riccardo LU and Hesslow, Germund LU (2018) In Scientific Reports 8(1).
Abstract

Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur.... (More)

Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur. Purkinje cells learned to respond with a firing pause to the conditional stimulus. Yet, there was no depression of parallel fibre excitation after training.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
8
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85054297619
ISSN
2045-2322
DOI
10.1038/s41598-018-32791-7
language
English
LU publication?
yes
id
2ea8494a-2a3c-47a2-b5a8-667dde645cd4
date added to LUP
2018-10-14 10:08:23
date last changed
2019-07-16 03:58:03
@article{2ea8494a-2a3c-47a2-b5a8-667dde645cd4,
  abstract     = {<p>Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur. Purkinje cells learned to respond with a firing pause to the conditional stimulus. Yet, there was no depression of parallel fibre excitation after training.</p>},
  articleno    = {14777},
  author       = {Johansson, Fredrik and Jirenhed, Dan Anders and Rasmussen, Anders and Zucca, Riccardo and Hesslow, Germund},
  issn         = {2045-2322},
  language     = {eng},
  month        = {12},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Absence of Parallel Fibre to Purkinje Cell LTD During Eyeblink Conditioning},
  url          = {http://dx.doi.org/10.1038/s41598-018-32791-7},
  volume       = {8},
  year         = {2018},
}