Absence of Parallel Fibre to Purkinje Cell LTD During Eyeblink Conditioning
Johansson, Fredrik LU ; Jirenhed, Dan Anders LU ; Rasmussen, Anders LU
; Zucca, Riccardo
LU
and Hesslow, Germund
LU
(2018)
In Scientific Reports
8(1).
- Abstract
Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur.... (More)
Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur. Purkinje cells learned to respond with a firing pause to the conditional stimulus. Yet, there was no depression of parallel fibre excitation after training.
(Less)
- author
- Johansson, Fredrik
LU
; Jirenhed, Dan Anders
LU
; Rasmussen, Anders
LU
; Zucca, Riccardo
LU
and Hesslow, Germund
LU
- organization
- publishing date
- 2018-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 8
- issue
- 1
- article number
- 14777
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85054297619
- pmid:30283004
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-018-32791-7
- language
- English
- LU publication?
- yes
- id
- 2ea8494a-2a3c-47a2-b5a8-667dde645cd4
- date added to LUP
- 2018-10-14 10:08:23
- date last changed
- 2024-06-24 21:52:03
@article{2ea8494a-2a3c-47a2-b5a8-667dde645cd4,
abstract = {{<p>Long-term depression (LTD) of parallel fibre/Purkinje cell synapses has been the favoured explanation for cerebellar motor learning such as classical eyeblink conditioning. Previous evidence against this interpretation has been contested. Here we wanted to test whether a classical conditioning protocol causes LTD. We applied a conditioning protocol, using a train of electrical pulses to the parallel fibres as the conditional stimulus. In order to rule out indirect effects caused by antidromic granule cell activation or output from Purkinje cells that might produce changes in Purkinje cell responsiveness, we focused the analysis on the first pulse in the conditional stimulus, that is, before any indirect effects would have time to occur. Purkinje cells learned to respond with a firing pause to the conditional stimulus. Yet, there was no depression of parallel fibre excitation after training.</p>}},
author = {{Johansson, Fredrik and Jirenhed, Dan Anders and Rasmussen, Anders and Zucca, Riccardo and Hesslow, Germund}},
issn = {{2045-2322}},
language = {{eng}},
month = {{12}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Absence of Parallel Fibre to Purkinje Cell LTD During Eyeblink Conditioning}},
url = {{http://dx.doi.org/10.1038/s41598-018-32791-7}},
doi = {{10.1038/s41598-018-32791-7}},
volume = {{8}},
year = {{2018}},
}