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FoxJ1-expressing cells contribute to neurogenesis in forebrain of adult rats: Evidence from in vivo electroporation combined with piggyBac transposon.

Devaraju, Karthikeyan LU ; Barnabé-Heider, Fanie ; Kokaia, Zaal LU and Lindvall, Olle LU (2013) In Experimental Cell Research 319(18). p.2790-2800
Abstract
Ependymal cells in the lateral ventricular wall are considered to be post-mitotic but can give rise to neuroblasts and astrocytes after stroke in adult mice due to insult-induced suppression of Notch signaling. The transcription factor FoxJ1, which has been used to characterize mouse ependymal cells, is also expressed by a subset of astrocytes. Cells expressing FoxJ1, which drives the expression of motile cilia, contribute to early postnatal neurogenesis in mouse olfactory bulb. The distribution and progeny of FoxJ1-expressing cells in rat forebrain are unknown. Here we show using immunohistochemistry that the overall majority of FoxJ1-expressing cells in the lateral ventricular wall of adult rats are ependymal cells with a minor... (More)
Ependymal cells in the lateral ventricular wall are considered to be post-mitotic but can give rise to neuroblasts and astrocytes after stroke in adult mice due to insult-induced suppression of Notch signaling. The transcription factor FoxJ1, which has been used to characterize mouse ependymal cells, is also expressed by a subset of astrocytes. Cells expressing FoxJ1, which drives the expression of motile cilia, contribute to early postnatal neurogenesis in mouse olfactory bulb. The distribution and progeny of FoxJ1-expressing cells in rat forebrain are unknown. Here we show using immunohistochemistry that the overall majority of FoxJ1-expressing cells in the lateral ventricular wall of adult rats are ependymal cells with a minor population being astrocytes. To allow for long-term fate mapping of FoxJ1-derived cells, we used the piggyBac system for in vivo gene transfer with electroporation. Using this method, we found that FoxJ1-expressing cells, presumably the astrocytes, give rise to neuroblasts and mature neurons in the olfactory bulb both in intact and stroke-damaged brain of adult rats. No significant contribution of FoxJ1-derived cells to stroke-induced striatal neurogenesis was detected. These data indicate that in the adult rat brain, FoxJ1-expressing cells contribute to the formation of new neurons in the olfactory bulb but are not involved in the cellular repair after stroke. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Experimental Cell Research
volume
319
issue
18
pages
2790 - 2800
publisher
Academic Press
external identifiers
  • wos:000326432700004
  • pmid:24075965
  • scopus:84886288357
  • pmid:24075965
ISSN
1090-2422
DOI
10.1016/j.yexcr.2013.08.028
language
English
LU publication?
yes
id
2fb3afbe-0ad9-46fa-8ca4-11c3b1106e50 (old id 4143838)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24075965?dopt=Abstract
date added to LUP
2016-04-01 10:53:27
date last changed
2020-01-12 06:02:23
@article{2fb3afbe-0ad9-46fa-8ca4-11c3b1106e50,
  abstract     = {Ependymal cells in the lateral ventricular wall are considered to be post-mitotic but can give rise to neuroblasts and astrocytes after stroke in adult mice due to insult-induced suppression of Notch signaling. The transcription factor FoxJ1, which has been used to characterize mouse ependymal cells, is also expressed by a subset of astrocytes. Cells expressing FoxJ1, which drives the expression of motile cilia, contribute to early postnatal neurogenesis in mouse olfactory bulb. The distribution and progeny of FoxJ1-expressing cells in rat forebrain are unknown. Here we show using immunohistochemistry that the overall majority of FoxJ1-expressing cells in the lateral ventricular wall of adult rats are ependymal cells with a minor population being astrocytes. To allow for long-term fate mapping of FoxJ1-derived cells, we used the piggyBac system for in vivo gene transfer with electroporation. Using this method, we found that FoxJ1-expressing cells, presumably the astrocytes, give rise to neuroblasts and mature neurons in the olfactory bulb both in intact and stroke-damaged brain of adult rats. No significant contribution of FoxJ1-derived cells to stroke-induced striatal neurogenesis was detected. These data indicate that in the adult rat brain, FoxJ1-expressing cells contribute to the formation of new neurons in the olfactory bulb but are not involved in the cellular repair after stroke.},
  author       = {Devaraju, Karthikeyan and Barnabé-Heider, Fanie and Kokaia, Zaal and Lindvall, Olle},
  issn         = {1090-2422},
  language     = {eng},
  number       = {18},
  pages        = {2790--2800},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {FoxJ1-expressing cells contribute to neurogenesis in forebrain of adult rats: Evidence from in vivo electroporation combined with piggyBac transposon.},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2013.08.028},
  doi          = {10.1016/j.yexcr.2013.08.028},
  volume       = {319},
  year         = {2013},
}