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High-Resolution Fluorescence Diffuse Optical Tomography Developed with Nonlinear Upconverting Nanoparticles

Xu, Can LU ; Svenmarker, Pontus LU ; Liu, Haichun LU ; Wu, Xia; Messing, Maria LU ; Wallenberg, Reine LU and Andersson-Engels, Stefan LU (2012) In ACS Nano 6(6). p.4788-4795
Abstract
Fluorescence diffuse optical tomography (FDOT) is an emerging biomedical imaging technique that can be used to localize and quantify deeply situated fluorescent molecules within tissues. However, the potential of this technique is currently limited by its poor spatial resolution. In this work, we demonstrate that the current resolution limit of FDOT can be breached by exploiting the nonlinear power-dependent optical emission property of upconverting nanoparticles doped with rare-earth elements. The rare-earth-doped core-shell nanoparticles, NaYF4:Yb3+/Tm3+@NaYF4 of hexagonal phase, are synthesized through a stoichiometric method, and optical characterization shows that the upconverting emission of the nanoparticles in tissues depends... (More)
Fluorescence diffuse optical tomography (FDOT) is an emerging biomedical imaging technique that can be used to localize and quantify deeply situated fluorescent molecules within tissues. However, the potential of this technique is currently limited by its poor spatial resolution. In this work, we demonstrate that the current resolution limit of FDOT can be breached by exploiting the nonlinear power-dependent optical emission property of upconverting nanoparticles doped with rare-earth elements. The rare-earth-doped core-shell nanoparticles, NaYF4:Yb3+/Tm3+@NaYF4 of hexagonal phase, are synthesized through a stoichiometric method, and optical characterization shows that the upconverting emission of the nanoparticles in tissues depends quadratically on the power of excitation. In addition, quantum-yield measurements of the emission from the synthesized nanoparticles are performed over a large range of excitation intensities, for both core and core-shell particles. The measurements show that the quantum yield of the 800 nm emission band of core-shell upconverting nanoparticles is 3.5% under an excitation intensity of 78 W/cm(2). The FDOT reconstruction experiments are carried out in a controlled environment using liquid tissue phantoms. The experiments show that the spatial resolution of the FDOT reconstruction images can be significantly improved by the use of the synthesized upconverting nanoparticles and break the current spatial resolution limits of FDOT images obtained from using conventional linear fluorophores as contrast agents. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
upconversion nanoparticles, bioimaging, quantum yield, resolution, diffuse imaging
in
ACS Nano
volume
6
issue
6
pages
4788 - 4795
publisher
The American Chemical Society
external identifiers
  • wos:000305661300026
  • scopus:84862864134
ISSN
1936-086X
DOI
10.1021/nn3015807
language
English
LU publication?
yes
id
0cc67da0-707d-47b3-9a52-680ba63287f9 (old id 3001727)
date added to LUP
2012-08-21 10:59:35
date last changed
2017-10-01 03:05:31
@article{0cc67da0-707d-47b3-9a52-680ba63287f9,
  abstract     = {Fluorescence diffuse optical tomography (FDOT) is an emerging biomedical imaging technique that can be used to localize and quantify deeply situated fluorescent molecules within tissues. However, the potential of this technique is currently limited by its poor spatial resolution. In this work, we demonstrate that the current resolution limit of FDOT can be breached by exploiting the nonlinear power-dependent optical emission property of upconverting nanoparticles doped with rare-earth elements. The rare-earth-doped core-shell nanoparticles, NaYF4:Yb3+/Tm3+@NaYF4 of hexagonal phase, are synthesized through a stoichiometric method, and optical characterization shows that the upconverting emission of the nanoparticles in tissues depends quadratically on the power of excitation. In addition, quantum-yield measurements of the emission from the synthesized nanoparticles are performed over a large range of excitation intensities, for both core and core-shell particles. The measurements show that the quantum yield of the 800 nm emission band of core-shell upconverting nanoparticles is 3.5% under an excitation intensity of 78 W/cm(2). The FDOT reconstruction experiments are carried out in a controlled environment using liquid tissue phantoms. The experiments show that the spatial resolution of the FDOT reconstruction images can be significantly improved by the use of the synthesized upconverting nanoparticles and break the current spatial resolution limits of FDOT images obtained from using conventional linear fluorophores as contrast agents.},
  author       = {Xu, Can and Svenmarker, Pontus and Liu, Haichun and Wu, Xia and Messing, Maria and Wallenberg, Reine and Andersson-Engels, Stefan},
  issn         = {1936-086X},
  keyword      = {upconversion nanoparticles,bioimaging,quantum yield,resolution,diffuse imaging},
  language     = {eng},
  number       = {6},
  pages        = {4788--4795},
  publisher    = {The American Chemical Society},
  series       = {ACS Nano},
  title        = {High-Resolution Fluorescence Diffuse Optical Tomography Developed with Nonlinear Upconverting Nanoparticles},
  url          = {http://dx.doi.org/10.1021/nn3015807},
  volume       = {6},
  year         = {2012},
}