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A comparison of peripheral nerve regeneration in acellular muscle and nerve autografts

Kerns, JM; Danielsen, Nils LU ; Zhao, Q; Lundborg, Göran LU and Kanje, M (2003) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00 37(4). p.193-200
Abstract
Regeneration of the rat sciatic nerve through acellular muscle and nerve autografts was evaluated 6-28 days postoperatively by the sensory pinch test, immunocytochemical staining for neurofilaments, and light and electron microscopy. Data points generated by the pinch test were plotted against postoperative time periods and by the use of regression analysis the initial delay period for muscle grafts was determined to 10.3 days. This value was similar to that previously published for acellular nerve grafts (9.5 days), but significantly longer than that for fresh nerve grafts (3.6 days). The calculated regeneration rate (slope of the regression line) for muscle grafts (1.8 mm/day) did not differ significantly ( p >0.05) from that... (More)
Regeneration of the rat sciatic nerve through acellular muscle and nerve autografts was evaluated 6-28 days postoperatively by the sensory pinch test, immunocytochemical staining for neurofilaments, and light and electron microscopy. Data points generated by the pinch test were plotted against postoperative time periods and by the use of regression analysis the initial delay period for muscle grafts was determined to 10.3 days. This value was similar to that previously published for acellular nerve grafts (9.5 days), but significantly longer than that for fresh nerve grafts (3.6 days). The calculated regeneration rate (slope of the regression line) for muscle grafts (1.8 mm/day) did not differ significantly ( p >0.05) from that calculated for acellular nerve grafts (2.1 mm/day) or for fresh nerve grafts (1.5 mm/day). The front of regenerating axons shown by axonal neurofilament staining confirmed the pinch test results. Both types of acellular grafts were repopulated with host non-neuronal cells and the muscle graft contained occasional ectopic muscle fibres. Remnants of graft basal laminae were evident at the ultrastructural level. These results indicate the suitability of either acellular muscle or nerve grafts for nerve repair despite their prolonged initial delay periods compared with conventional fresh nerve grafts. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acellular, regeneration, autografts, nerve, muscle
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00
volume
37
issue
4
pages
193 - 200
publisher
Taylor & Francis
external identifiers
  • wos:000185137300001
  • pmid:14582750
  • scopus:0041911185
ISSN
1651-2073
DOI
language
English
LU publication?
yes
id
84a3f3b8-5c7c-4647-9987-df66a22834f8 (old id 301556)
date added to LUP
2007-08-03 12:13:05
date last changed
2018-05-29 09:51:58
@article{84a3f3b8-5c7c-4647-9987-df66a22834f8,
  abstract     = {Regeneration of the rat sciatic nerve through acellular muscle and nerve autografts was evaluated 6-28 days postoperatively by the sensory pinch test, immunocytochemical staining for neurofilaments, and light and electron microscopy. Data points generated by the pinch test were plotted against postoperative time periods and by the use of regression analysis the initial delay period for muscle grafts was determined to 10.3 days. This value was similar to that previously published for acellular nerve grafts (9.5 days), but significantly longer than that for fresh nerve grafts (3.6 days). The calculated regeneration rate (slope of the regression line) for muscle grafts (1.8 mm/day) did not differ significantly ( p >0.05) from that calculated for acellular nerve grafts (2.1 mm/day) or for fresh nerve grafts (1.5 mm/day). The front of regenerating axons shown by axonal neurofilament staining confirmed the pinch test results. Both types of acellular grafts were repopulated with host non-neuronal cells and the muscle graft contained occasional ectopic muscle fibres. Remnants of graft basal laminae were evident at the ultrastructural level. These results indicate the suitability of either acellular muscle or nerve grafts for nerve repair despite their prolonged initial delay periods compared with conventional fresh nerve grafts.},
  author       = {Kerns, JM and Danielsen, Nils and Zhao, Q and Lundborg, Göran and Kanje, M},
  issn         = {1651-2073},
  keyword      = {acellular,regeneration,autografts,nerve,muscle},
  language     = {eng},
  number       = {4},
  pages        = {193--200},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00},
  title        = {A comparison of peripheral nerve regeneration in acellular muscle and nerve autografts},
  url          = {http://dx.doi.org/},
  volume       = {37},
  year         = {2003},
}