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Changes in tissue optical properties due to radio-frequency ablation of myocardium

Swartling, Johannes LU ; Pålsson, Sara LU ; Platonov, Pyotr LU ; Olsson, SB and Andersson-Engels, Stefan LU (2003) In Medical & Biological Engineering & Computing 41(4). p.403-409
Abstract
The optical properties of pig heart tissue were measured after in vivo ablation therapy had been performed during open-heart surgery. In vitro samples of normal and ablated tissue were subjected to measurements with an optically integrating sphere set-up in the region 470-900nm. Three independent measurements were made: total transmittance, total reflectance and collimated transmittance, which made it possible to extract the absorption and scattering coefficients and the scattering anisotropy factor g, using an inverse Monte Carlo model. Between 470 and 700nm, only the reduced scattering coefficient and absorption could be evaluated. The absorption spectra were fitted to known tissue chromophore spectra, so that the concentrations of... (More)
The optical properties of pig heart tissue were measured after in vivo ablation therapy had been performed during open-heart surgery. In vitro samples of normal and ablated tissue were subjected to measurements with an optically integrating sphere set-up in the region 470-900nm. Three independent measurements were made: total transmittance, total reflectance and collimated transmittance, which made it possible to extract the absorption and scattering coefficients and the scattering anisotropy factor g, using an inverse Monte Carlo model. Between 470 and 700nm, only the reduced scattering coefficient and absorption could be evaluated. The absorption spectra were fitted to known tissue chromophore spectra, so that the concentrations of haemoglobin and myoglobin could be estimated. The reduced scattering coefficient was compared with Mie computations to provide Mie equivalent average radii. Most of the absorption was from myoglobin, whereas haemoglobin absorption was negligible. Metmyoglobin was formed in the ablated tissue, which could yield a spectral signature to distinguish the ablated tissue with a simple optical probe to monitor the ablation therapy. The reduced scattering coefficient increased by, on average, 50% in the ablated tissue, which corresponded to a slight decrease in the Mie equivalent radius. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
arrhythmia, cardiology, Monte Carlo simulations, scattering, tissue optics, spectroscopy
in
Medical & Biological Engineering & Computing
volume
41
issue
4
pages
403 - 409
publisher
Springer
external identifiers
  • wos:000184231800005
  • pmid:12892362
  • scopus:0041307311
ISSN
0140-0118
DOI
language
English
LU publication?
yes
id
8618ef7d-95c1-4337-b974-79528438f1ff (old id 305908)
date added to LUP
2007-09-23 09:51:26
date last changed
2018-06-10 04:33:46
@article{8618ef7d-95c1-4337-b974-79528438f1ff,
  abstract     = {The optical properties of pig heart tissue were measured after in vivo ablation therapy had been performed during open-heart surgery. In vitro samples of normal and ablated tissue were subjected to measurements with an optically integrating sphere set-up in the region 470-900nm. Three independent measurements were made: total transmittance, total reflectance and collimated transmittance, which made it possible to extract the absorption and scattering coefficients and the scattering anisotropy factor g, using an inverse Monte Carlo model. Between 470 and 700nm, only the reduced scattering coefficient and absorption could be evaluated. The absorption spectra were fitted to known tissue chromophore spectra, so that the concentrations of haemoglobin and myoglobin could be estimated. The reduced scattering coefficient was compared with Mie computations to provide Mie equivalent average radii. Most of the absorption was from myoglobin, whereas haemoglobin absorption was negligible. Metmyoglobin was formed in the ablated tissue, which could yield a spectral signature to distinguish the ablated tissue with a simple optical probe to monitor the ablation therapy. The reduced scattering coefficient increased by, on average, 50% in the ablated tissue, which corresponded to a slight decrease in the Mie equivalent radius.},
  author       = {Swartling, Johannes and Pålsson, Sara and Platonov, Pyotr and Olsson, SB and Andersson-Engels, Stefan},
  issn         = {0140-0118},
  keyword      = {arrhythmia,cardiology,Monte Carlo simulations,scattering,tissue optics,spectroscopy},
  language     = {eng},
  number       = {4},
  pages        = {403--409},
  publisher    = {Springer},
  series       = {Medical & Biological Engineering & Computing},
  title        = {Changes in tissue optical properties due to radio-frequency ablation of myocardium},
  url          = {http://dx.doi.org/},
  volume       = {41},
  year         = {2003},
}