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Water- and temperature-dependence of DNA damage and repair in the fruticose lichen Cladonia arbuscula ssp mitis exposed to UV-B radiation

Buffoni Hall, Roberta LU ; Paulsson, M; Duncan, K; Tobin, AK; Widell, Susanne LU and Bornman, Janet LU (2003) In Physiologia Plantarum 118(3). p.371-379
Abstract
The induction of cyclobutane pyrimidine dimers (CPDs) by ultraviolet-B radiation (UV-B, 280-315 nm) and repair mechanisms were studied in the lichen Cladonia arbuscula ssp. mitis exposed to different temperatures and water status conditions. In addition, the development and repair of CPDs were studied in relation to the different developmental stages of the lichen thallus podetial branches. Air-dried lichen thalli exposed to UV-B radiation combined with relatively high visible light (HL, 800 mumol m(-2) s(-1); 400-700 nm) for 7 days showed a progressive increase of CPDs with no substantial repair, although HL was present during and after irradiation with UV-B. Fully hydrated lichen thalli, that had not been previously exposed to UV-B... (More)
The induction of cyclobutane pyrimidine dimers (CPDs) by ultraviolet-B radiation (UV-B, 280-315 nm) and repair mechanisms were studied in the lichen Cladonia arbuscula ssp. mitis exposed to different temperatures and water status conditions. In addition, the development and repair of CPDs were studied in relation to the different developmental stages of the lichen thallus podetial branches. Air-dried lichen thalli exposed to UV-B radiation combined with relatively high visible light (HL, 800 mumol m(-2) s(-1); 400-700 nm) for 7 days showed a progressive increase of CPDs with no substantial repair, although HL was present during and after irradiation with UV-B. Fully hydrated lichen thalli, that had not been previously exposed to UV-B radiation for 7 days, were given short-term UV-B radiation treatment at 25degreesC, and accumulated DNA lesions in the form of CPDs, with repair occurring when they were exposed to photoreactivating conditions (2 h of 300 mumol m(-2)s(-1), 400-700 nm). A different pattern was observed when fully hydrated thalli were exposed to short-term UV-B radiation at 2degreesC, in comparison with exposure at 25degreesC. High levels of CPDs were induced at 2degreesC under UV-B irradiation, without significant repair under subsequent photoreactivating light. Likewise, when PAR (300 mumol m(-2)s(-1) ) and UV-B radiation were given simultaneously, the CPD levels were not lowered. Throughout all experiments the youngest, less differentiated parts of the lichen thallus - namely 'tips', according to our arbitrary subdivision - were the parts showing the highest levels of CPD accumulation and the lowest levels of repair in comparison with the older thallus tissue ('stems'). Thus the experiments showed that Cladonia arbuscula ssp. mitis is sensitive to UV-B irradiation in the air-dried state and is not able to completely repair the damage caused by the radiation. Furthermore, temperature plays a role in the DNA damage repairing capacity of this lichen, since even when fully hydrated, C. arbuscula ssp. mitis did not repair DNA damage at the low temperatures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physiologia Plantarum
volume
118
issue
3
pages
371 - 379
publisher
Wiley Online Library
external identifiers
  • wos:000183604600009
  • scopus:0038350302
ISSN
0031-9317
DOI
10.1034/j.1399-3054.2003.00130.x
language
English
LU publication?
yes
id
360e5182-714c-4b90-988c-04419e244200 (old id 308359)
date added to LUP
2007-09-03 14:13:08
date last changed
2018-01-07 08:49:45
@article{360e5182-714c-4b90-988c-04419e244200,
  abstract     = {The induction of cyclobutane pyrimidine dimers (CPDs) by ultraviolet-B radiation (UV-B, 280-315 nm) and repair mechanisms were studied in the lichen Cladonia arbuscula ssp. mitis exposed to different temperatures and water status conditions. In addition, the development and repair of CPDs were studied in relation to the different developmental stages of the lichen thallus podetial branches. Air-dried lichen thalli exposed to UV-B radiation combined with relatively high visible light (HL, 800 mumol m(-2) s(-1); 400-700 nm) for 7 days showed a progressive increase of CPDs with no substantial repair, although HL was present during and after irradiation with UV-B. Fully hydrated lichen thalli, that had not been previously exposed to UV-B radiation for 7 days, were given short-term UV-B radiation treatment at 25degreesC, and accumulated DNA lesions in the form of CPDs, with repair occurring when they were exposed to photoreactivating conditions (2 h of 300 mumol m(-2)s(-1), 400-700 nm). A different pattern was observed when fully hydrated thalli were exposed to short-term UV-B radiation at 2degreesC, in comparison with exposure at 25degreesC. High levels of CPDs were induced at 2degreesC under UV-B irradiation, without significant repair under subsequent photoreactivating light. Likewise, when PAR (300 mumol m(-2)s(-1) ) and UV-B radiation were given simultaneously, the CPD levels were not lowered. Throughout all experiments the youngest, less differentiated parts of the lichen thallus - namely 'tips', according to our arbitrary subdivision - were the parts showing the highest levels of CPD accumulation and the lowest levels of repair in comparison with the older thallus tissue ('stems'). Thus the experiments showed that Cladonia arbuscula ssp. mitis is sensitive to UV-B irradiation in the air-dried state and is not able to completely repair the damage caused by the radiation. Furthermore, temperature plays a role in the DNA damage repairing capacity of this lichen, since even when fully hydrated, C. arbuscula ssp. mitis did not repair DNA damage at the low temperatures.},
  author       = {Buffoni Hall, Roberta and Paulsson, M and Duncan, K and Tobin, AK and Widell, Susanne and Bornman, Janet},
  issn         = {0031-9317},
  language     = {eng},
  number       = {3},
  pages        = {371--379},
  publisher    = {Wiley Online Library},
  series       = {Physiologia Plantarum},
  title        = {Water- and temperature-dependence of DNA damage and repair in the fruticose lichen Cladonia arbuscula ssp mitis exposed to UV-B radiation},
  url          = {http://dx.doi.org/10.1034/j.1399-3054.2003.00130.x},
  volume       = {118},
  year         = {2003},
}