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Initiation of the decorin glycosaminoglycan chain in the endoplasmic reticulum-Golgi intermediate compartment

Jönsson, Mats LU ; Eklund, Erik LU ; Fransson, Lars-Åke LU and Oldberg, Åke LU (2003) In Journal of Biological Chemistry 278(24). p.21415-21420
Abstract
We have transiently expressed decorin with a C- terminal KDEL endoplasmic reticulum retention signal peptide in COS- 7 cells to study initiation of galactosaminoglycan synthesis in the endoplasmic reticulum- Golgi intermediate compartment. All decorin- KDEL molecules were substituted with N- linked oligosaccharides sensitive to endoglycosidase H, indicating that the core protein was located proximal to the medial- Golgi. O-Linked glycosylation was only initiated in a minor fraction of the molecules. The O- linked saccharides were characterized by gel filtration after stepwise degradations using chondroitin ABC/ AC-I lyases, beta1 - 3- glycuronidase, beta-galactosidase, and alkaline phosphatase. The major O- linked saccharide was the... (More)
We have transiently expressed decorin with a C- terminal KDEL endoplasmic reticulum retention signal peptide in COS- 7 cells to study initiation of galactosaminoglycan synthesis in the endoplasmic reticulum- Golgi intermediate compartment. All decorin- KDEL molecules were substituted with N- linked oligosaccharides sensitive to endoglycosidase H, indicating that the core protein was located proximal to the medial- Golgi. O-Linked glycosylation was only initiated in a minor fraction of the molecules. The O- linked saccharides were characterized by gel filtration after stepwise degradations using chondroitin ABC/ AC-I lyases, beta1 - 3- glycuronidase, beta-galactosidase, and alkaline phosphatase. The major O- linked saccharide was the linkage region pentasaccharide GalNAcbeta1-4GlcUAbeta1-3Galbeta1-3Galbeta1-4-Xyl- 2- phosphate, demonstrating initiation of chondroitin synthesis in the endoplasmic reticulum- Golgi intermediate compartment. In the presence of brefeldin A, partial elongation of a chondroitin chain took place, indicating retrieval of polymerases but not of sulfotransferases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
278
issue
24
pages
21415 - 21420
publisher
ASBMB
external identifiers
  • wos:000183354200015
  • scopus:0038498824
ISSN
1083-351X
DOI
10.1074/jbc.M210977200
language
English
LU publication?
yes
id
f422e802-d2a0-4c26-8eb8-5084f060b33e (old id 308889)
date added to LUP
2007-09-13 14:43:05
date last changed
2018-10-03 10:34:22
@article{f422e802-d2a0-4c26-8eb8-5084f060b33e,
  abstract     = {We have transiently expressed decorin with a C- terminal KDEL endoplasmic reticulum retention signal peptide in COS- 7 cells to study initiation of galactosaminoglycan synthesis in the endoplasmic reticulum- Golgi intermediate compartment. All decorin- KDEL molecules were substituted with N- linked oligosaccharides sensitive to endoglycosidase H, indicating that the core protein was located proximal to the medial- Golgi. O-Linked glycosylation was only initiated in a minor fraction of the molecules. The O- linked saccharides were characterized by gel filtration after stepwise degradations using chondroitin ABC/ AC-I lyases, beta1 - 3- glycuronidase, beta-galactosidase, and alkaline phosphatase. The major O- linked saccharide was the linkage region pentasaccharide GalNAcbeta1-4GlcUAbeta1-3Galbeta1-3Galbeta1-4-Xyl- 2- phosphate, demonstrating initiation of chondroitin synthesis in the endoplasmic reticulum- Golgi intermediate compartment. In the presence of brefeldin A, partial elongation of a chondroitin chain took place, indicating retrieval of polymerases but not of sulfotransferases.},
  author       = {Jönsson, Mats and Eklund, Erik and Fransson, Lars-Åke and Oldberg, Åke},
  issn         = {1083-351X},
  language     = {eng},
  number       = {24},
  pages        = {21415--21420},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Initiation of the decorin glycosaminoglycan chain in the endoplasmic reticulum-Golgi intermediate compartment},
  url          = {http://dx.doi.org/10.1074/jbc.M210977200},
  volume       = {278},
  year         = {2003},
}