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Local growth factors are beneficial for the autonomic reinnervation of transplanted islets in rats

Kvist Reimer, Martina LU ; Mokshagundam, SP; Wyler, K; Sundler, Frank LU ; Ahrén, Bo LU and Stagner, JI (2003) In Pancreas 26(4). p.392-397
Abstract
Introduction: Transplanted islets, being avascular and denervated, receive blood vessels and nerves from the recipient. Reinnervation may account in part for the normalization of islet function in islet transplants. Whether reinnervation is possible to augment is not known. Aims and Methodology: To explore whether reinnervation of transplanted islets is augmented by local addition of growth factors to the graft, syngeneic islets were transplanted to the pancreas of streptozotocin-diabetic Lewis rats with or without pellets locally releasing nerve growth factor (NGF) and vascular endothelial growth factor (VEGF), alone or in combination. The pellets released growth factors for 14 days at a rate of 20 ng/day. After 7 weeks, pancreatic tissue... (More)
Introduction: Transplanted islets, being avascular and denervated, receive blood vessels and nerves from the recipient. Reinnervation may account in part for the normalization of islet function in islet transplants. Whether reinnervation is possible to augment is not known. Aims and Methodology: To explore whether reinnervation of transplanted islets is augmented by local addition of growth factors to the graft, syngeneic islets were transplanted to the pancreas of streptozotocin-diabetic Lewis rats with or without pellets locally releasing nerve growth factor (NGF) and vascular endothelial growth factor (VEGF), alone or in combination. The pellets released growth factors for 14 days at a rate of 20 ng/day. After 7 weeks, pancreatic tissue was processed for immunofluorescence of insulin and the neural markers neuropeptide Y (NPY) and tyrosine hydroxylase (TH). Results: Islets were larger and more numerous after treatment with NGF (p = 0.024) and with NGF in combination with VEGF (p = 0.044). Similarly, immunostaining for TH and the C-terminal flanking peptide of NPY (C-PON) was more pronounced after treatment with NGF in combination with VEGF than in controls (both p < 0.05). Conclusion: Local growth factor treatment has a beneficial effect on autonomic reinnervation as well as islet integrity and survival of the graft after islet transplantation in rats. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
transplantation, rat, neuropeptides, nerve growth factor, graft, autonomic nerves, diabetes, vascular endothelial growth factor
in
Pancreas
volume
26
issue
4
pages
392 - 397
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000182676200014
  • pmid:12717274
  • scopus:0037730407
ISSN
0885-3177
language
English
LU publication?
yes
id
e01cdeab-fe61-48bb-b95b-3a1d3435dfa9 (old id 311921)
date added to LUP
2007-09-20 12:35:11
date last changed
2018-01-07 05:10:40
@article{e01cdeab-fe61-48bb-b95b-3a1d3435dfa9,
  abstract     = {Introduction: Transplanted islets, being avascular and denervated, receive blood vessels and nerves from the recipient. Reinnervation may account in part for the normalization of islet function in islet transplants. Whether reinnervation is possible to augment is not known. Aims and Methodology: To explore whether reinnervation of transplanted islets is augmented by local addition of growth factors to the graft, syngeneic islets were transplanted to the pancreas of streptozotocin-diabetic Lewis rats with or without pellets locally releasing nerve growth factor (NGF) and vascular endothelial growth factor (VEGF), alone or in combination. The pellets released growth factors for 14 days at a rate of 20 ng/day. After 7 weeks, pancreatic tissue was processed for immunofluorescence of insulin and the neural markers neuropeptide Y (NPY) and tyrosine hydroxylase (TH). Results: Islets were larger and more numerous after treatment with NGF (p = 0.024) and with NGF in combination with VEGF (p = 0.044). Similarly, immunostaining for TH and the C-terminal flanking peptide of NPY (C-PON) was more pronounced after treatment with NGF in combination with VEGF than in controls (both p &lt; 0.05). Conclusion: Local growth factor treatment has a beneficial effect on autonomic reinnervation as well as islet integrity and survival of the graft after islet transplantation in rats.},
  author       = {Kvist Reimer, Martina and Mokshagundam, SP and Wyler, K and Sundler, Frank and Ahrén, Bo and Stagner, JI},
  issn         = {0885-3177},
  keyword      = {transplantation,rat,neuropeptides,nerve growth factor,graft,autonomic nerves,diabetes,vascular endothelial growth factor},
  language     = {eng},
  number       = {4},
  pages        = {392--397},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Pancreas},
  title        = {Local growth factors are beneficial for the autonomic reinnervation of transplanted islets in rats},
  volume       = {26},
  year         = {2003},
}