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DNA with double-chained amphiphilic counterions and its interaction with lecithin.

Krivtsov, Alexey LU ; Bilalov, Azat LU ; Olsson, Ulf LU and Lindman, Björn LU (2012) In Langmuir 28(38). p.13698-13704
Abstract
Complex salts of double-stranded DNA with amphiphilic counterions offer novel opportunities for studies of DNA-lipid interactions. Here the effect of the hydrophobicity of the amphiphilic counterion is in focus. For this purpose, double stranded DNA with didodecyldimethylammonium ions as counterions, DDADNA, is prepared and investigated with respect to microstructure. In particular, in order to monitor the interactions with phospholipids, the phase diagram of the DDADNA/lecithin/water system is determined and compared with the previously determined phase diagram with single alkyl chain counterions, dodecyltrimethylammonium, DTA. In both systems, there is a formation of lamellar and reverse hexagonal phases, where hydrated DNA is sandwiched... (More)
Complex salts of double-stranded DNA with amphiphilic counterions offer novel opportunities for studies of DNA-lipid interactions. Here the effect of the hydrophobicity of the amphiphilic counterion is in focus. For this purpose, double stranded DNA with didodecyldimethylammonium ions as counterions, DDADNA, is prepared and investigated with respect to microstructure. In particular, in order to monitor the interactions with phospholipids, the phase diagram of the DDADNA/lecithin/water system is determined and compared with the previously determined phase diagram with single alkyl chain counterions, dodecyltrimethylammonium, DTA. In both systems, there is a formation of lamellar and reverse hexagonal phases, where hydrated DNA is sandwiched between bilayers or forms the core of reverse cylindrical micelles, respectively. However, whereas the lecithin lamellar phase can incorporate large amounts of DDADNA, there is in the case of the single chain surfactant, DTADNA, a transition to a bicontinuous cubic phase at higher DTADNA concentrations. The general appearance of the phase diagrams, and in particular the role of counterion hydrophobicity, can be rationalized in a simple geometric model. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
28
issue
38
pages
13698 - 13704
publisher
The American Chemical Society
external identifiers
  • wos:000309040800019
  • pmid:22943435
  • scopus:84866647348
ISSN
0743-7463
DOI
10.1021/la302720d
language
English
LU publication?
yes
id
98e15160-e436-4d85-addd-1873de8dc128 (old id 3124401)
date added to LUP
2012-10-10 09:48:30
date last changed
2017-09-24 03:25:59
@article{98e15160-e436-4d85-addd-1873de8dc128,
  abstract     = {Complex salts of double-stranded DNA with amphiphilic counterions offer novel opportunities for studies of DNA-lipid interactions. Here the effect of the hydrophobicity of the amphiphilic counterion is in focus. For this purpose, double stranded DNA with didodecyldimethylammonium ions as counterions, DDADNA, is prepared and investigated with respect to microstructure. In particular, in order to monitor the interactions with phospholipids, the phase diagram of the DDADNA/lecithin/water system is determined and compared with the previously determined phase diagram with single alkyl chain counterions, dodecyltrimethylammonium, DTA. In both systems, there is a formation of lamellar and reverse hexagonal phases, where hydrated DNA is sandwiched between bilayers or forms the core of reverse cylindrical micelles, respectively. However, whereas the lecithin lamellar phase can incorporate large amounts of DDADNA, there is in the case of the single chain surfactant, DTADNA, a transition to a bicontinuous cubic phase at higher DTADNA concentrations. The general appearance of the phase diagrams, and in particular the role of counterion hydrophobicity, can be rationalized in a simple geometric model.},
  author       = {Krivtsov, Alexey and Bilalov, Azat and Olsson, Ulf and Lindman, Björn},
  issn         = {0743-7463},
  language     = {eng},
  number       = {38},
  pages        = {13698--13704},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {DNA with double-chained amphiphilic counterions and its interaction with lecithin.},
  url          = {http://dx.doi.org/10.1021/la302720d},
  volume       = {28},
  year         = {2012},
}