Advanced

Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes.

Andersson, Sofia A LU ; Olsson, Anders H LU ; Esguerra, Jonathan L S; Heimann, Emilia LU ; Ladenvall, Claes LU ; Edlund, Anna LU ; Salehi, S Albert LU ; Taneera, Jalal LU ; Degerman, Eva LU and Groop, Leif LU , et al. (2012) In Molecular and Cellular Endocrinology 364(1-2). p.36-45
Abstract
Reduced insulin release has been linked to defect exocytosis in β-cells. However, whether expression of genes suggested to be involved in the exocytotic process (exocytotic genes) is altered in pancreatic islets from patients with type 2 diabetes (T2D), and correlate to insulin secretion, needs to be further investigated. Analysing expression levels of 23 exocytotic genes using microarray revealed reduced expression of five genes in human T2D islets (χ(2)=13.25; p<0.001). Gene expression of STX1A, SYT4, SYT7, SYT11, SYT13, SNAP25 and STXBP1 correlated negatively to in vivo measurements of HbA1c levels and positively to glucose stimulated insulin secretion (GSIS) in vitro in human islets. STX1A, SYT4 and SYT11 protein levels... (More)
Reduced insulin release has been linked to defect exocytosis in β-cells. However, whether expression of genes suggested to be involved in the exocytotic process (exocytotic genes) is altered in pancreatic islets from patients with type 2 diabetes (T2D), and correlate to insulin secretion, needs to be further investigated. Analysing expression levels of 23 exocytotic genes using microarray revealed reduced expression of five genes in human T2D islets (χ(2)=13.25; p<0.001). Gene expression of STX1A, SYT4, SYT7, SYT11, SYT13, SNAP25 and STXBP1 correlated negatively to in vivo measurements of HbA1c levels and positively to glucose stimulated insulin secretion (GSIS) in vitro in human islets. STX1A, SYT4 and SYT11 protein levels correspondingly decreased in human T2D islets. Moreover, silencing of SYT4 and SYT13 reduced GSIS in INS1-832/13 cells. Our data support that reduced expression of exocytotic genes contributes to impaired insulin secretion, and suggest decreased expression of these genes as part of T2D pathogenesis. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Endocrinology
volume
364
issue
1-2
pages
36 - 45
publisher
Elsevier
external identifiers
  • wos:000311017200003
  • pmid:22939844
  • scopus:84867518459
ISSN
1872-8057
DOI
10.1016/j.mce.2012.08.009
language
English
LU publication?
yes
id
186001c6-d9f6-495d-b922-cb50ea0c2a4d (old id 3124493)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22939844?dopt=Abstract
date added to LUP
2012-10-02 17:08:06
date last changed
2017-10-08 03:17:16
@article{186001c6-d9f6-495d-b922-cb50ea0c2a4d,
  abstract     = {Reduced insulin release has been linked to defect exocytosis in β-cells. However, whether expression of genes suggested to be involved in the exocytotic process (exocytotic genes) is altered in pancreatic islets from patients with type 2 diabetes (T2D), and correlate to insulin secretion, needs to be further investigated. Analysing expression levels of 23 exocytotic genes using microarray revealed reduced expression of five genes in human T2D islets (χ(2)=13.25; p&lt;0.001). Gene expression of STX1A, SYT4, SYT7, SYT11, SYT13, SNAP25 and STXBP1 correlated negatively to in vivo measurements of HbA1c levels and positively to glucose stimulated insulin secretion (GSIS) in vitro in human islets. STX1A, SYT4 and SYT11 protein levels correspondingly decreased in human T2D islets. Moreover, silencing of SYT4 and SYT13 reduced GSIS in INS1-832/13 cells. Our data support that reduced expression of exocytotic genes contributes to impaired insulin secretion, and suggest decreased expression of these genes as part of T2D pathogenesis.},
  author       = {Andersson, Sofia A and Olsson, Anders H and Esguerra, Jonathan L S and Heimann, Emilia and Ladenvall, Claes and Edlund, Anna and Salehi, S Albert and Taneera, Jalal and Degerman, Eva and Groop, Leif and Ling, Charlotte and Eliasson, Lena},
  issn         = {1872-8057},
  language     = {eng},
  number       = {1-2},
  pages        = {36--45},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Endocrinology},
  title        = {Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes.},
  url          = {http://dx.doi.org/10.1016/j.mce.2012.08.009},
  volume       = {364},
  year         = {2012},
}