Aerosolization and recovery of viable murine norovirus in an experimental setup
(2020) In Scientific Reports 10(1).- Abstract
Noroviruses are the major cause for viral acute gastroenteritis in the world. Despite the existing infection prevention strategies in hospitals, the disease continues to spread and causes extensive and numerous outbreaks. Hence, there is a need to investigate the possibility of airborne transmission of norovirus. In this study, we developed an experimental setup for studies on the infectivity of aerosolized murine norovirus (MNV), a model for the human norovirus. Two aerosol generation principles were evaluated: bubble bursting, a common natural aerosolization mechanism, and nebulization, a common aerosolization technique in laboratory studies. The aerosolization setup was characterized by physical and viral dilution factors, generated... (More)
Noroviruses are the major cause for viral acute gastroenteritis in the world. Despite the existing infection prevention strategies in hospitals, the disease continues to spread and causes extensive and numerous outbreaks. Hence, there is a need to investigate the possibility of airborne transmission of norovirus. In this study, we developed an experimental setup for studies on the infectivity of aerosolized murine norovirus (MNV), a model for the human norovirus. Two aerosol generation principles were evaluated: bubble bursting, a common natural aerosolization mechanism, and nebulization, a common aerosolization technique in laboratory studies. The aerosolization setup was characterized by physical and viral dilution factors, generated aerosol particle size distributions, and the viral infectivity after aerosolization. We found a lower physical dilution factor when using the nebulization generator than with the bubble bursting generator. The viral dilution factor of the system was higher than the physical dilution; however, when comparing the physical and viral dilution factors, bubble bursting generation was more efficient. The infectivity per virus was similar using either generation principle, suggesting that the generation itself had a minor impact on MNV infectivity and that instead, the effect of drying in air could be a major reason for infectivity losses.
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- author
- Alsved, Malin LU ; Widell, Anders LU ; Dahlin, Henrik ; Karlson, Sara LU ; Medstrand, Patrik LU and Löndahl, Jakob LU
- organization
-
- Ergonomics and Aerosol Technology
- MERGE: ModElling the Regional and Global Earth system
- NanoLund: Centre for Nanoscience
- Metalund
- Clinical Microbiology, Malmö (research group)
- Division of Medical Microbiology
- HIV-1 and HIV-2 host interactions (research group)
- Clinical Virology, Malmö (research group)
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 10
- issue
- 1
- article number
- 15941
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85091717323
- pmid:32994471
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-020-72932-5
- language
- English
- LU publication?
- yes
- id
- 31434860-5cf8-4d00-899d-161099966534
- date added to LUP
- 2020-10-22 12:53:07
- date last changed
- 2024-04-03 15:41:34
@article{31434860-5cf8-4d00-899d-161099966534, abstract = {{<p>Noroviruses are the major cause for viral acute gastroenteritis in the world. Despite the existing infection prevention strategies in hospitals, the disease continues to spread and causes extensive and numerous outbreaks. Hence, there is a need to investigate the possibility of airborne transmission of norovirus. In this study, we developed an experimental setup for studies on the infectivity of aerosolized murine norovirus (MNV), a model for the human norovirus. Two aerosol generation principles were evaluated: bubble bursting, a common natural aerosolization mechanism, and nebulization, a common aerosolization technique in laboratory studies. The aerosolization setup was characterized by physical and viral dilution factors, generated aerosol particle size distributions, and the viral infectivity after aerosolization. We found a lower physical dilution factor when using the nebulization generator than with the bubble bursting generator. The viral dilution factor of the system was higher than the physical dilution; however, when comparing the physical and viral dilution factors, bubble bursting generation was more efficient. The infectivity per virus was similar using either generation principle, suggesting that the generation itself had a minor impact on MNV infectivity and that instead, the effect of drying in air could be a major reason for infectivity losses.</p>}}, author = {{Alsved, Malin and Widell, Anders and Dahlin, Henrik and Karlson, Sara and Medstrand, Patrik and Löndahl, Jakob}}, issn = {{2045-2322}}, language = {{eng}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Aerosolization and recovery of viable murine norovirus in an experimental setup}}, url = {{http://dx.doi.org/10.1038/s41598-020-72932-5}}, doi = {{10.1038/s41598-020-72932-5}}, volume = {{10}}, year = {{2020}}, }