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Immunohistochemical studies on the distribution of albumin, fibrinogen, fibronectin, IgG and collagen around PTFE and titanium implants

Rosengren, Agneta LU ; Johansson, B R LU ; Danielsen, N LU ; Thomsen, P. and Ericson, L E (1996) In Biomaterials 17(18). p.86-1779
Abstract

Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a... (More)

Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a cryosubstitution method combined with postembedding immunohistochemistry, a light microscopic analysis was allowed. The selected proteins had an apparently varying distribution in the implant-close tissue and their distribution changed during the follow-up period. There was also a difference in the distribution pattern for each protein around titanium and PTFE implants. Insertion of the c.p. titanium implants elicited an inflammatory reaction in many respects similar to a normal wound healing response, while the PTFE implants caused a more pronounced, persistent inflammation.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Abdominal Muscles, Animals, Biocompatible Materials, Collagen, Fibrinogen, Fibronectins, Immunoglobulin G, Immunohistochemistry, Male, Muscle Fibers, Skeletal, Polytetrafluoroethylene, Prostheses and Implants, Rats, Rats, Sprague-Dawley, Serum Albumin, Titanium, Journal Article, Research Support, Non-U.S. Gov't
in
Biomaterials
volume
17
issue
18
pages
8 pages
publisher
Elsevier
external identifiers
  • pmid:8879516
  • scopus:0030250736
ISSN
0142-9612
language
English
LU publication?
yes
id
31ebeef3-1f4a-48c1-906d-4954b5b8eadb
date added to LUP
2017-10-13 13:36:59
date last changed
2024-10-14 15:03:23
@article{31ebeef3-1f4a-48c1-906d-4954b5b8eadb,
  abstract     = {{<p>Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a cryosubstitution method combined with postembedding immunohistochemistry, a light microscopic analysis was allowed. The selected proteins had an apparently varying distribution in the implant-close tissue and their distribution changed during the follow-up period. There was also a difference in the distribution pattern for each protein around titanium and PTFE implants. Insertion of the c.p. titanium implants elicited an inflammatory reaction in many respects similar to a normal wound healing response, while the PTFE implants caused a more pronounced, persistent inflammation.</p>}},
  author       = {{Rosengren, Agneta and Johansson, B R and Danielsen, N and Thomsen, P. and Ericson, L E}},
  issn         = {{0142-9612}},
  keywords     = {{Abdominal Muscles; Animals; Biocompatible Materials; Collagen; Fibrinogen; Fibronectins; Immunoglobulin G; Immunohistochemistry; Male; Muscle Fibers, Skeletal; Polytetrafluoroethylene; Prostheses and Implants; Rats; Rats, Sprague-Dawley; Serum Albumin; Titanium; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{18}},
  pages        = {{86--1779}},
  publisher    = {{Elsevier}},
  series       = {{Biomaterials}},
  title        = {{Immunohistochemical studies on the distribution of albumin, fibrinogen, fibronectin, IgG and collagen around PTFE and titanium implants}},
  volume       = {{17}},
  year         = {{1996}},
}