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Immunohistochemical studies on the distribution of albumin, fibrinogen, fibronectin, IgG and collagen around PTFE and titanium implants

Rosengren, Agneta LU ; Johansson, B R LU ; Danielsen, N LU ; Thomsen, P. and Ericson, L E (1996) In Biomaterials 17(18). p.86-1779
Abstract

Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a... (More)

Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a cryosubstitution method combined with postembedding immunohistochemistry, a light microscopic analysis was allowed. The selected proteins had an apparently varying distribution in the implant-close tissue and their distribution changed during the follow-up period. There was also a difference in the distribution pattern for each protein around titanium and PTFE implants. Insertion of the c.p. titanium implants elicited an inflammatory reaction in many respects similar to a normal wound healing response, while the PTFE implants caused a more pronounced, persistent inflammation.

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published
subject
keywords
Abdominal Muscles, Animals, Biocompatible Materials, Collagen, Fibrinogen, Fibronectins, Immunoglobulin G, Immunohistochemistry, Male, Muscle Fibers, Skeletal, Polytetrafluoroethylene, Prostheses and Implants, Rats, Rats, Sprague-Dawley, Serum Albumin, Titanium, Journal Article, Research Support, Non-U.S. Gov't
in
Biomaterials
volume
17
issue
18
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:0030250736
ISSN
0142-9612
language
English
LU publication?
yes
id
31ebeef3-1f4a-48c1-906d-4954b5b8eadb
date added to LUP
2017-10-13 13:36:59
date last changed
2017-11-29 12:16:57
@article{31ebeef3-1f4a-48c1-906d-4954b5b8eadb,
  abstract     = {<p>Time-dependent distribution of extracellular proteins (albumin, fibrinogen, fibronectin, collagen-I and IgG) in the interface zone between implant and soft tissue has been investigated utilizing a recently developed method. Commercially pure (c.p.) titanium and polytetrafluoroethylene (PTFE) implants were inserted in the abdominal wall of rats for 1, 6 and 12 weeks followed by a mild fixation, cryoprotection, rapid freezing in LN2-cooled propane, cryosubstitution and low-temperature infiltration with UV curing of the methacrylate LR-Gold. Before sectioning, the bulk part of the titanium was removed by an electrolytical dissolution technique (electropolishing), while the PTFE implants were removed by a fracture technique. Employing a cryosubstitution method combined with postembedding immunohistochemistry, a light microscopic analysis was allowed. The selected proteins had an apparently varying distribution in the implant-close tissue and their distribution changed during the follow-up period. There was also a difference in the distribution pattern for each protein around titanium and PTFE implants. Insertion of the c.p. titanium implants elicited an inflammatory reaction in many respects similar to a normal wound healing response, while the PTFE implants caused a more pronounced, persistent inflammation.</p>},
  author       = {Rosengren, Agneta and Johansson, B R and Danielsen, N and Thomsen, P. and Ericson, L E},
  issn         = {0142-9612},
  keyword      = {Abdominal Muscles,Animals,Biocompatible Materials,Collagen,Fibrinogen,Fibronectins,Immunoglobulin G,Immunohistochemistry,Male,Muscle Fibers, Skeletal,Polytetrafluoroethylene,Prostheses and Implants,Rats,Rats, Sprague-Dawley,Serum Albumin,Titanium,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {18},
  pages        = {86--1779},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Immunohistochemical studies on the distribution of albumin, fibrinogen, fibronectin, IgG and collagen around PTFE and titanium implants},
  volume       = {17},
  year         = {1996},
}