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GDH biosensor based off-line capillary immunoassay for alkylphenols and their ethoxylates

Rose, A; Nistor, Catalin LU ; Emnéus, Jenny LU ; Pfeiffer, D and Wollenberger, U (2002) In Biosensors & Bioelectronics 17(11-12). p.1033-1043
Abstract
The application of a quinoprotein glucose dehydrogenase modified thick-film sensor as label detector in a capillary immunoassay (CIA) for xenoestrogens is presented. The detection of the alkylphenols and their ethoxylates is based on the competition between the analyte and tracer molecules for the binding sites of anti-alkylphenol ethoxylate antibodies. This assay is performed off-line in small disposable PVC capillaries coated with immobilized antibodies. This format allows the combination of the assay with a small portable device potentially useful for on-site environmental monitoring. Beside high amplification the utilization of beta-galactosidase as enzyme label allows the direct combination with a GDH biosensor at optimal pH... (More)
The application of a quinoprotein glucose dehydrogenase modified thick-film sensor as label detector in a capillary immunoassay (CIA) for xenoestrogens is presented. The detection of the alkylphenols and their ethoxylates is based on the competition between the analyte and tracer molecules for the binding sites of anti-alkylphenol ethoxylate antibodies. This assay is performed off-line in small disposable PVC capillaries coated with immobilized antibodies. This format allows the combination of the assay with a small portable device potentially useful for on-site environmental monitoring. Beside high amplification the utilization of beta-galactosidase as enzyme label allows the direct combination with a GDH biosensor at optimal pH conditions. The bioelectrocatalytic properties of this biosensor offer an additional amplification and thus allow a very sensitive quantification of 4-aminophenol, generated by the beta-galactosidase. Detection limits of the analytes in the mug/l range were obtained, while other phenolics and surfactants showed no or very little cross reactivity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-galactosidase, portable device, GDH biosensor, capillary immunoassay, electrochemical detection, alkylphenols, alkylphenol, ethoxylates
in
Biosensors & Bioelectronics
volume
17
issue
11-12
pages
1033 - 1043
publisher
Elsevier
external identifiers
  • wos:000178791900016
  • pmid:12392953
  • scopus:0036883527
ISSN
1873-4235
DOI
10.1016/S0956-5663(02)00096-9
language
English
LU publication?
yes
id
13e6ead3-eb51-4cd0-b189-7c049c9a6a26 (old id 324841)
date added to LUP
2007-11-09 08:51:07
date last changed
2017-01-01 06:57:30
@article{13e6ead3-eb51-4cd0-b189-7c049c9a6a26,
  abstract     = {The application of a quinoprotein glucose dehydrogenase modified thick-film sensor as label detector in a capillary immunoassay (CIA) for xenoestrogens is presented. The detection of the alkylphenols and their ethoxylates is based on the competition between the analyte and tracer molecules for the binding sites of anti-alkylphenol ethoxylate antibodies. This assay is performed off-line in small disposable PVC capillaries coated with immobilized antibodies. This format allows the combination of the assay with a small portable device potentially useful for on-site environmental monitoring. Beside high amplification the utilization of beta-galactosidase as enzyme label allows the direct combination with a GDH biosensor at optimal pH conditions. The bioelectrocatalytic properties of this biosensor offer an additional amplification and thus allow a very sensitive quantification of 4-aminophenol, generated by the beta-galactosidase. Detection limits of the analytes in the mug/l range were obtained, while other phenolics and surfactants showed no or very little cross reactivity.},
  author       = {Rose, A and Nistor, Catalin and Emnéus, Jenny and Pfeiffer, D and Wollenberger, U},
  issn         = {1873-4235},
  keyword      = {beta-galactosidase,portable device,GDH biosensor,capillary immunoassay,electrochemical detection,alkylphenols,alkylphenol,ethoxylates},
  language     = {eng},
  number       = {11-12},
  pages        = {1033--1043},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {GDH biosensor based off-line capillary immunoassay for alkylphenols and their ethoxylates},
  url          = {http://dx.doi.org/10.1016/S0956-5663(02)00096-9},
  volume       = {17},
  year         = {2002},
}