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Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization

Hamacher, T; Becker, J; Gárdonyi, Márk LU ; Hahn-Hägerdal, Bärbel LU and Boles, E (2002) In Microbiology1994-01-01+01:00 148. p.2783-2788
Abstract
For an economically feasible production of ethanol from plant biomass by microbial cells, the fermentation of xylose is important. As xylose uptake might be a limiting step for xylose fermentation by recombinant xylose-utilizing Saccharomyces cerevisiae cells a study of xylose uptake was performed. After deletion of all of the 18 hexose-transporter genes, the ability of the cells to take up and to grow on xylose was lost. Reintroduction of individual hexose-transporter genes in this strain revealed that at intermediate xylose concentrations the yeast high- and intermediate-affinity transporters Hxt4, Hxt5, Hxt7 and Ga12 are important xylose-transporting proteins. Several heterologous monosaccharide transporters from bacteria and plant... (More)
For an economically feasible production of ethanol from plant biomass by microbial cells, the fermentation of xylose is important. As xylose uptake might be a limiting step for xylose fermentation by recombinant xylose-utilizing Saccharomyces cerevisiae cells a study of xylose uptake was performed. After deletion of all of the 18 hexose-transporter genes, the ability of the cells to take up and to grow on xylose was lost. Reintroduction of individual hexose-transporter genes in this strain revealed that at intermediate xylose concentrations the yeast high- and intermediate-affinity transporters Hxt4, Hxt5, Hxt7 and Ga12 are important xylose-transporting proteins. Several heterologous monosaccharide transporters from bacteria and plant cells did not confer sufficient uptake activity to restore growth on xylose. Overexpression of the xylose-transporting proteins in a xylose-utilizing PUA yeast strain did not result in faster growth on xylose under aerobic conditions nor did it enhance the xylose fermentation rate under anaerobic conditions. The results of this study suggest that xylose uptake does not determine the xylose flux under the conditions and in the yeast strains investigated. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fermentation, xylose, glucose uptake, Saccharomyces cerevisiae, xylose uptake, heterologous expression
in
Microbiology1994-01-01+01:00
volume
148
pages
2783 - 2788
publisher
MAIK Nauka/Interperiodica
external identifiers
  • wos:000178070500013
  • pmid:12213924
  • scopus:0036738179
ISSN
1465-2080
language
English
LU publication?
yes
id
75093e2a-8f7b-4daf-91fd-6726a3973d53 (old id 328537)
alternative location
http://mic.sgmjournals.org/cgi/content/abstract/148/9/2783
date added to LUP
2007-08-23 10:35:05
date last changed
2017-10-22 03:55:44
@article{75093e2a-8f7b-4daf-91fd-6726a3973d53,
  abstract     = {For an economically feasible production of ethanol from plant biomass by microbial cells, the fermentation of xylose is important. As xylose uptake might be a limiting step for xylose fermentation by recombinant xylose-utilizing Saccharomyces cerevisiae cells a study of xylose uptake was performed. After deletion of all of the 18 hexose-transporter genes, the ability of the cells to take up and to grow on xylose was lost. Reintroduction of individual hexose-transporter genes in this strain revealed that at intermediate xylose concentrations the yeast high- and intermediate-affinity transporters Hxt4, Hxt5, Hxt7 and Ga12 are important xylose-transporting proteins. Several heterologous monosaccharide transporters from bacteria and plant cells did not confer sufficient uptake activity to restore growth on xylose. Overexpression of the xylose-transporting proteins in a xylose-utilizing PUA yeast strain did not result in faster growth on xylose under aerobic conditions nor did it enhance the xylose fermentation rate under anaerobic conditions. The results of this study suggest that xylose uptake does not determine the xylose flux under the conditions and in the yeast strains investigated.},
  author       = {Hamacher, T and Becker, J and Gárdonyi, Márk and Hahn-Hägerdal, Bärbel and Boles, E},
  issn         = {1465-2080},
  keyword      = {fermentation,xylose,glucose uptake,Saccharomyces cerevisiae,xylose uptake,heterologous expression},
  language     = {eng},
  pages        = {2783--2788},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology1994-01-01+01:00},
  title        = {Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization},
  volume       = {148},
  year         = {2002},
}