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Protein-surfactant interactions at solid surfaces

Arnebrant, Thomas and Wahlgren, Marie C LU orcid (1995) In ACS Symposium Series 602. p.239-255
Abstract
Effects of surfactants on protein adsorption are reviewed. Differences between removal of preadsorbed proteins (elutability) and competitive adsorption are discussed and simple models are suggested. It can be concluded that surfactants may interact through solubilization or replacement mechanisms depending on surfactant- surface interactions and surfactant- protein binding. Solubilization requires complex formation between protein and surfactant, and the replacement adsorption of the surfactant to the surface. As for protein adsorption, one of the most important properties affecting the elutability appears to be the conformational stability. Differences between a competitive situation and addition of surfactant after adsorption of the... (More)
Effects of surfactants on protein adsorption are reviewed. Differences between removal of preadsorbed proteins (elutability) and competitive adsorption are discussed and simple models are suggested. It can be concluded that surfactants may interact through solubilization or replacement mechanisms depending on surfactant- surface interactions and surfactant- protein binding. Solubilization requires complex formation between protein and surfactant, and the replacement adsorption of the surfactant to the surface. As for protein adsorption, one of the most important properties affecting the elutability appears to be the conformational stability. Differences between a competitive situation and addition of surfactant after adsorption of the protein are suggested to originate from alteration in surface activity of protein-surfactant complexes formed in solution as compared to pure protein, the difference in diffusivity of surfactants and protein, and time dependent conformational changes of the protein. (Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Proteins at Interfaces II Fundamentals and Applications
series title
ACS Symposium Series
editor
Horbett1, Thomas A. Horbett1 and L. Brash, John
volume
602
pages
17 pages
publisher
The American Chemical Society (ACS)
ISBN
9780841233041
9780841215276
DOI
10.1021/bk-1995-0602.ch017
language
English
LU publication?
yes
id
32eff569-5129-4d80-8d8b-86c586525f6c
date added to LUP
2016-04-15 19:24:53
date last changed
2019-10-04 14:38:44
@inbook{32eff569-5129-4d80-8d8b-86c586525f6c,
  abstract     = {{Effects of surfactants on protein adsorption are reviewed. Differences between removal of preadsorbed proteins (elutability) and competitive adsorption are discussed and simple models are suggested. It can be concluded that surfactants may interact through solubilization or replacement mechanisms depending on surfactant- surface interactions and surfactant- protein binding. Solubilization requires complex formation between protein and surfactant, and the replacement adsorption of the surfactant to the surface. As for protein adsorption, one of the most important properties affecting the elutability appears to be the conformational stability. Differences between a competitive situation and addition of surfactant after adsorption of the protein are suggested to originate from alteration in surface activity of protein-surfactant complexes formed in solution as compared to pure protein, the difference in diffusivity of surfactants and protein, and time dependent conformational changes of the protein.}},
  author       = {{Arnebrant, Thomas and Wahlgren, Marie C}},
  booktitle    = {{Proteins at Interfaces II Fundamentals and Applications}},
  editor       = {{Horbett1, Thomas A. Horbett1 and L. Brash, John}},
  isbn         = {{9780841233041}},
  language     = {{eng}},
  pages        = {{239--255}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Symposium Series}},
  title        = {{Protein-surfactant interactions at solid surfaces}},
  url          = {{http://dx.doi.org/10.1021/bk-1995-0602.ch017}},
  doi          = {{10.1021/bk-1995-0602.ch017}},
  volume       = {{602}},
  year         = {{1995}},
}