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Effects of fat, beef and fiber in diets on activities of sphingomyelinase, ceramidase and caspase-3 in rat colonic mucosa

Yang, Liping LU ; Mutanen, M; Cheng, Yajun LU and Duan, Rui-Dong LU (2002) In Medical Principles and Practice 11(3). p.150-156
Abstract
Objective: The present study investigates the activity changes of sphingomyelinase (SMase), ceramidase and caspase-3 in colonic mucosa of rats induced by high fat and red meat diets. Method. For a period of 3 weeks, rats were fed protein without fat (control), high fat only, high fat with beef, and high fat with fiber (cellulose) diets. The fat content (22.4%) was constant in the three high fat diets. Then, the colonic mucosae were scraped and homogenized and the activities of SMase, ceramidase and caspase-3 determined. Results: Compared to the control diet, the fat diet and fat with beef diet reduced intestinal alkaline SMase by 80 and 84%, respectively; ceramidase activity by 60 and 92%, respectively, and caspase-3 activity by 40 and... (More)
Objective: The present study investigates the activity changes of sphingomyelinase (SMase), ceramidase and caspase-3 in colonic mucosa of rats induced by high fat and red meat diets. Method. For a period of 3 weeks, rats were fed protein without fat (control), high fat only, high fat with beef, and high fat with fiber (cellulose) diets. The fat content (22.4%) was constant in the three high fat diets. Then, the colonic mucosae were scraped and homogenized and the activities of SMase, ceramidase and caspase-3 determined. Results: Compared to the control diet, the fat diet and fat with beef diet reduced intestinal alkaline SMase by 80 and 84%, respectively; ceramidase activity by 60 and 92%, respectively, and caspase-3 activity by 40 and 75%, respectively. The activities of acid and neutral SMases were also decreased by fat and fat with beef diets but to a smaller extent than those of alkaline SMase. Supplement of fiber in the fat diet had no effect on the changes of alkaline SMase activity but prevented fat-induced decreases in acid and neutral SMase activities and partially prevented those of ceramidase and caspase-3 activities. The activity of intestinal alkaline phosphatase was not changed by any of the diets. Conclusion: Fat, beef and fiber significantly affect the enzymes responsible for sphingomyelin metabolism and apoptosis in the colon. The effects may have implications in colonic tumorigenesis related to dietary factors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fiber, red meat, fat, ceramidase, sphingomyelinase, caspase-3, colon
in
Medical Principles and Practice
volume
11
issue
3
pages
150 - 156
publisher
Karger
external identifiers
  • wos:000177535400007
  • scopus:0036053298
ISSN
1011-7571
DOI
10.1159/000063247
language
English
LU publication?
yes
id
33af3992-3c94-452e-ad55-e5d571b164d3 (old id 330940)
date added to LUP
2007-11-19 13:07:07
date last changed
2017-01-01 05:01:25
@article{33af3992-3c94-452e-ad55-e5d571b164d3,
  abstract     = {Objective: The present study investigates the activity changes of sphingomyelinase (SMase), ceramidase and caspase-3 in colonic mucosa of rats induced by high fat and red meat diets. Method. For a period of 3 weeks, rats were fed protein without fat (control), high fat only, high fat with beef, and high fat with fiber (cellulose) diets. The fat content (22.4%) was constant in the three high fat diets. Then, the colonic mucosae were scraped and homogenized and the activities of SMase, ceramidase and caspase-3 determined. Results: Compared to the control diet, the fat diet and fat with beef diet reduced intestinal alkaline SMase by 80 and 84%, respectively; ceramidase activity by 60 and 92%, respectively, and caspase-3 activity by 40 and 75%, respectively. The activities of acid and neutral SMases were also decreased by fat and fat with beef diets but to a smaller extent than those of alkaline SMase. Supplement of fiber in the fat diet had no effect on the changes of alkaline SMase activity but prevented fat-induced decreases in acid and neutral SMase activities and partially prevented those of ceramidase and caspase-3 activities. The activity of intestinal alkaline phosphatase was not changed by any of the diets. Conclusion: Fat, beef and fiber significantly affect the enzymes responsible for sphingomyelin metabolism and apoptosis in the colon. The effects may have implications in colonic tumorigenesis related to dietary factors.},
  author       = {Yang, Liping and Mutanen, M and Cheng, Yajun and Duan, Rui-Dong},
  issn         = {1011-7571},
  keyword      = {fiber,red meat,fat,ceramidase,sphingomyelinase,caspase-3,colon},
  language     = {eng},
  number       = {3},
  pages        = {150--156},
  publisher    = {Karger},
  series       = {Medical Principles and Practice},
  title        = {Effects of fat, beef and fiber in diets on activities of sphingomyelinase, ceramidase and caspase-3 in rat colonic mucosa},
  url          = {http://dx.doi.org/10.1159/000063247},
  volume       = {11},
  year         = {2002},
}