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Ethanol production from enzymatic hydrolysates of sugarcane bagasse using recombinant xylose-utilising Saccharomyces cerevisiae

Martin, C; Galbe, Mats LU ; Wahlbom, Fredrik LU ; Hahn-Hägerdal, Bärbel LU and Jonsson, Leif (2002) In Enzyme and Microbial Technology 31(3). p.274-282
Abstract
Sugarcane bagasse was pre-treated by steam explosion at 205 and 215degreesC and hydrolysed with cellulolytic enzymes. The hydrolysates were subjected to enzymatic detoxification by treatment with the phenoloxidase laccase and to chemical detoxification by overliming. Approximately 80% of the phenolic compounds were specifically removed by the laccase treatment. Overliming partially removed the phenolic compounds, but also other fermentation inhibitors such as acetic acid, furfural and 5-hydroxy-methyl-furfural. The hydrolysates were fermented with the recombinant xylose-utilising Saccharomyces cerevisiae laboratory strain TMB 3001, a CEN.PK derivative with over-expressed xylulokinase activity and expressing the xylose reductase and xylitol... (More)
Sugarcane bagasse was pre-treated by steam explosion at 205 and 215degreesC and hydrolysed with cellulolytic enzymes. The hydrolysates were subjected to enzymatic detoxification by treatment with the phenoloxidase laccase and to chemical detoxification by overliming. Approximately 80% of the phenolic compounds were specifically removed by the laccase treatment. Overliming partially removed the phenolic compounds, but also other fermentation inhibitors such as acetic acid, furfural and 5-hydroxy-methyl-furfural. The hydrolysates were fermented with the recombinant xylose-utilising Saccharomyces cerevisiae laboratory strain TMB 3001, a CEN.PK derivative with over-expressed xylulokinase activity and expressing the xylose reductase and xylitol dehydrogenase of Pichia stipitis, and the S. cerevisiae strain ATCC 9658 1, isolated from a spent sulphite liquor fermentation plant. The fermentative performance of the lab strain in undetoxified hydrolysate was better than the performance of the industrial strain. An almost two-fold increase of the specific productivity of the strain TMB 3001 in the detoxified hydrolysates compared to the undetoxified hydrolysates was observed. The ethanol yield in the fermentation of the hydrolysate detoxified by overliming was 0.18 g/g dry bagasse, whereas it reached only 0.13 g/g dry bagasse in the undetoxified hydrolysate. Partial xylose utilisation with low xylitol formation was observed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
inhibitors, fermentation, bagasse, ethanol, lignocellulose, detoxification, xylose
in
Enzyme and Microbial Technology
volume
31
issue
3
pages
274 - 282
publisher
Elsevier
external identifiers
  • wos:000177498900012
  • scopus:0037008398
ISSN
0141-0229
DOI
10.1016/S0141-0229(02)00112-6
language
English
LU publication?
yes
id
7f4ac355-93a7-4c0b-8eb1-35883e17d785 (old id 331048)
date added to LUP
2007-11-16 09:46:22
date last changed
2017-11-19 03:27:49
@article{7f4ac355-93a7-4c0b-8eb1-35883e17d785,
  abstract     = {Sugarcane bagasse was pre-treated by steam explosion at 205 and 215degreesC and hydrolysed with cellulolytic enzymes. The hydrolysates were subjected to enzymatic detoxification by treatment with the phenoloxidase laccase and to chemical detoxification by overliming. Approximately 80% of the phenolic compounds were specifically removed by the laccase treatment. Overliming partially removed the phenolic compounds, but also other fermentation inhibitors such as acetic acid, furfural and 5-hydroxy-methyl-furfural. The hydrolysates were fermented with the recombinant xylose-utilising Saccharomyces cerevisiae laboratory strain TMB 3001, a CEN.PK derivative with over-expressed xylulokinase activity and expressing the xylose reductase and xylitol dehydrogenase of Pichia stipitis, and the S. cerevisiae strain ATCC 9658 1, isolated from a spent sulphite liquor fermentation plant. The fermentative performance of the lab strain in undetoxified hydrolysate was better than the performance of the industrial strain. An almost two-fold increase of the specific productivity of the strain TMB 3001 in the detoxified hydrolysates compared to the undetoxified hydrolysates was observed. The ethanol yield in the fermentation of the hydrolysate detoxified by overliming was 0.18 g/g dry bagasse, whereas it reached only 0.13 g/g dry bagasse in the undetoxified hydrolysate. Partial xylose utilisation with low xylitol formation was observed.},
  author       = {Martin, C and Galbe, Mats and Wahlbom, Fredrik and Hahn-Hägerdal, Bärbel and Jonsson, Leif},
  issn         = {0141-0229},
  keyword      = {inhibitors,fermentation,bagasse,ethanol,lignocellulose,detoxification,xylose},
  language     = {eng},
  number       = {3},
  pages        = {274--282},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Ethanol production from enzymatic hydrolysates of sugarcane bagasse using recombinant xylose-utilising Saccharomyces cerevisiae},
  url          = {http://dx.doi.org/10.1016/S0141-0229(02)00112-6},
  volume       = {31},
  year         = {2002},
}