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Supercritical fluid extraction of a lignocellulosic hydrolysate of spruce for detoxification and to facilitate analysis of inhibitors

Persson, Per LU ; Larsson, S; Jonsson, Leif; Nilvebrant, NO; Sivik, Björn LU ; Munteanu, Florentina LU ; Thörneby, Lars LU and Gorton, Lo LU (2002) In Biotechnology and Bioengineering 79(6). p.694-700
Abstract
This work describes a novel approach to detoxify lignocellulosic hydrolysates and facilitate the analysis of inhibitory compounds, namely supercritical fluid extraction (SFE). The efficiency of the fermentation of lignocellulosic dilute-acid hydrolysates depends upon the composition of the hydrolysate and the organism used. Furthermore, it has been shown that inhibitors in the hydrolysate reduce the fermentation yield. This knowledge has given rise to the need to identify and remove the inhibiting compounds. Sample clean-up or work-up steps, to provide a clean and concentrated sample for the analytical system, facilitate the characterization of inhibitors, or indeed any compound in the hydrolysates. Removal of inhibitors was performed with... (More)
This work describes a novel approach to detoxify lignocellulosic hydrolysates and facilitate the analysis of inhibitory compounds, namely supercritical fluid extraction (SFE). The efficiency of the fermentation of lignocellulosic dilute-acid hydrolysates depends upon the composition of the hydrolysate and the organism used. Furthermore, it has been shown that inhibitors in the hydrolysate reduce the fermentation yield. This knowledge has given rise to the need to identify and remove the inhibiting compounds. Sample clean-up or work-up steps, to provide a clean and concentrated sample for the analytical system, facilitate the characterization of inhibitors, or indeed any compound in the hydrolysates. Removal of inhibitors was performed with countercurrent flow supercritical fluid extraction of liquid hydrolysates. Three different groups of inhibitors (furan derivatives, phenolic compounds, and aliphatic acids) and sugars were subsequently analyzed in the hydrolysate, extracted hydrolysate, and extract. The effect of the SFE treatment was examined with respect to fermentability with Saccharomyces cerevisiae. Not only did the extraction provide a clean and concentrated sample (extract) for analysis, but also a hydrolysate with increased fermentability as well as lower concentrations of inhibitors such as phenolics and furan derivatives. (C) 2002 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fermentation, supercritical fluid extraction (SFE), detoxification, lignocellulosic hydrolysates, inhibitors, softwood
in
Biotechnology and Bioengineering
volume
79
issue
6
pages
694 - 700
publisher
John Wiley & Sons
external identifiers
  • wos:000177549400013
  • pmid:12209817
  • scopus:0037144091
ISSN
1097-0290
DOI
10.1002/bit.10324
language
English
LU publication?
yes
id
05b2777c-17c4-49e0-b6de-69a4d53d4cb7 (old id 331100)
date added to LUP
2007-11-08 14:28:37
date last changed
2017-10-22 03:42:02
@article{05b2777c-17c4-49e0-b6de-69a4d53d4cb7,
  abstract     = {This work describes a novel approach to detoxify lignocellulosic hydrolysates and facilitate the analysis of inhibitory compounds, namely supercritical fluid extraction (SFE). The efficiency of the fermentation of lignocellulosic dilute-acid hydrolysates depends upon the composition of the hydrolysate and the organism used. Furthermore, it has been shown that inhibitors in the hydrolysate reduce the fermentation yield. This knowledge has given rise to the need to identify and remove the inhibiting compounds. Sample clean-up or work-up steps, to provide a clean and concentrated sample for the analytical system, facilitate the characterization of inhibitors, or indeed any compound in the hydrolysates. Removal of inhibitors was performed with countercurrent flow supercritical fluid extraction of liquid hydrolysates. Three different groups of inhibitors (furan derivatives, phenolic compounds, and aliphatic acids) and sugars were subsequently analyzed in the hydrolysate, extracted hydrolysate, and extract. The effect of the SFE treatment was examined with respect to fermentability with Saccharomyces cerevisiae. Not only did the extraction provide a clean and concentrated sample (extract) for analysis, but also a hydrolysate with increased fermentability as well as lower concentrations of inhibitors such as phenolics and furan derivatives. (C) 2002 Wiley Periodicals, Inc.},
  author       = {Persson, Per and Larsson, S and Jonsson, Leif and Nilvebrant, NO and Sivik, Björn and Munteanu, Florentina and Thörneby, Lars and Gorton, Lo},
  issn         = {1097-0290},
  keyword      = {fermentation,supercritical fluid extraction (SFE),detoxification,lignocellulosic hydrolysates,inhibitors,softwood},
  language     = {eng},
  number       = {6},
  pages        = {694--700},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Supercritical fluid extraction of a lignocellulosic hydrolysate of spruce for detoxification and to facilitate analysis of inhibitors},
  url          = {http://dx.doi.org/10.1002/bit.10324},
  volume       = {79},
  year         = {2002},
}