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Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis

Delgado, Osvaldo LU ; Martinez, MA; Abate, CM and Sineriz, F (2002) In Biotechnology Letters 24(15). p.1285-1290
Abstract
An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PCR, Zymomonas mobilis, gfpuv, alcohol dehydrogenase, expression vector
in
Biotechnology Letters
volume
24
issue
15
pages
1285 - 1290
publisher
Springer
external identifiers
  • wos:000176804800012
  • scopus:0036332032
ISSN
1573-6776
DOI
10.1023/A:1016266110391
language
English
LU publication?
yes
id
5ee18abd-45af-48c9-a252-07c978bc4fef (old id 333874)
date added to LUP
2007-08-16 08:27:58
date last changed
2017-01-01 07:03:46
@article{5ee18abd-45af-48c9-a252-07c978bc4fef,
  abstract     = {An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth.},
  author       = {Delgado, Osvaldo and Martinez, MA and Abate, CM and Sineriz, F},
  issn         = {1573-6776},
  keyword      = {PCR,Zymomonas mobilis,gfpuv,alcohol dehydrogenase,expression vector},
  language     = {eng},
  number       = {15},
  pages        = {1285--1290},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis},
  url          = {http://dx.doi.org/10.1023/A:1016266110391},
  volume       = {24},
  year         = {2002},
}