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At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

Eckstein, M; Sesing, M; Kragl, U and Adlercreutz, Patrick LU (2002) In Biotechnology Letters 24(11). p.867-872
Abstract
The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
water activity, alpha-chymotrypsin, ionic liquids
in
Biotechnology Letters
volume
24
issue
11
pages
867 - 872
publisher
Springer
external identifiers
  • wos:000175752100003
  • scopus:0036273739
ISSN
1573-6776
DOI
10.1023/A:1015564608261
language
English
LU publication?
yes
id
014ecc11-1a14-4228-94f5-3ed36bb1d0c2 (old id 337279)
date added to LUP
2007-08-02 16:31:15
date last changed
2017-11-12 04:01:21
@article{014ecc11-1a14-4228-94f5-3ed36bb1d0c2,
  abstract     = {The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.},
  author       = {Eckstein, M and Sesing, M and Kragl, U and Adlercreutz, Patrick},
  issn         = {1573-6776},
  keyword      = {water activity,alpha-chymotrypsin,ionic liquids},
  language     = {eng},
  number       = {11},
  pages        = {867--872},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents},
  url          = {http://dx.doi.org/10.1023/A:1015564608261},
  volume       = {24},
  year         = {2002},
}