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Process development of oxygen-demanding reactions utilizing a simple design with parallel glass tube reactors - Evaluated using Gluconobacter oxydans (DSM 24525)

Grey, Carl LU ; Viloria-Cols, Maria LU ; Jungvid, Hans and Adlercreutz, Patrick LU (2012) In Biocatalysis and Biotransformation 30(5-6). p.441-445
Abstract
To investigate the possibility of using simple glass tubes as reactors for oxygen-demanding reactions, a setup was assembled to study the initial rate of conversion of glycerol to dihydroxyacetone (DHA) using Gluconobacter oxydans. Several parallel 10 mL glass tubes were incubated in a temperature-controlled shaker. The concentration of DHA was determined using a fast spectrophotometric HPLC-based method that could process 3 samples/min. It was shown that the obtained results were reproducible and the reaction rates remained constant throughout the reaction. Further, the system reached a high volumetric activity of 15.48 g DHA L-1 h(-1) consuming 86 mmol L-1 h(-1) oxygen before the system became mass-transfer limited, indicating a high... (More)
To investigate the possibility of using simple glass tubes as reactors for oxygen-demanding reactions, a setup was assembled to study the initial rate of conversion of glycerol to dihydroxyacetone (DHA) using Gluconobacter oxydans. Several parallel 10 mL glass tubes were incubated in a temperature-controlled shaker. The concentration of DHA was determined using a fast spectrophotometric HPLC-based method that could process 3 samples/min. It was shown that the obtained results were reproducible and the reaction rates remained constant throughout the reaction. Further, the system reached a high volumetric activity of 15.48 g DHA L-1 h(-1) consuming 86 mmol L-1 h(-1) oxygen before the system became mass-transfer limited, indicating a high diffusion of oxygen. It was concluded that the reactor system is well suited for process development where the requirement for oxygen is high and that the assay developed can be used to determine the initial rate of DHA production. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bioreactor, oxygen, activity assay, glycerol, dihydroxyacetone, Gluconobacter oxydans
in
Biocatalysis and Biotransformation
volume
30
issue
5-6
pages
441 - 445
publisher
Taylor & Francis
external identifiers
  • wos:000311680300001
  • scopus:84870546663
ISSN
1024-2422
DOI
10.3109/10242422.2012.740019
language
English
LU publication?
yes
id
2da9d3c8-b40e-4122-b96a-9b823195a56f (old id 3388502)
date added to LUP
2013-01-30 13:36:38
date last changed
2017-01-01 03:17:16
@article{2da9d3c8-b40e-4122-b96a-9b823195a56f,
  abstract     = {To investigate the possibility of using simple glass tubes as reactors for oxygen-demanding reactions, a setup was assembled to study the initial rate of conversion of glycerol to dihydroxyacetone (DHA) using Gluconobacter oxydans. Several parallel 10 mL glass tubes were incubated in a temperature-controlled shaker. The concentration of DHA was determined using a fast spectrophotometric HPLC-based method that could process 3 samples/min. It was shown that the obtained results were reproducible and the reaction rates remained constant throughout the reaction. Further, the system reached a high volumetric activity of 15.48 g DHA L-1 h(-1) consuming 86 mmol L-1 h(-1) oxygen before the system became mass-transfer limited, indicating a high diffusion of oxygen. It was concluded that the reactor system is well suited for process development where the requirement for oxygen is high and that the assay developed can be used to determine the initial rate of DHA production.},
  author       = {Grey, Carl and Viloria-Cols, Maria and Jungvid, Hans and Adlercreutz, Patrick},
  issn         = {1024-2422},
  keyword      = {bioreactor,oxygen,activity assay,glycerol,dihydroxyacetone,Gluconobacter oxydans},
  language     = {eng},
  number       = {5-6},
  pages        = {441--445},
  publisher    = {Taylor & Francis},
  series       = {Biocatalysis and Biotransformation},
  title        = {Process development of oxygen-demanding reactions utilizing a simple design with parallel glass tube reactors - Evaluated using Gluconobacter oxydans (DSM 24525)},
  url          = {http://dx.doi.org/10.3109/10242422.2012.740019},
  volume       = {30},
  year         = {2012},
}