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In-field monitoring of cleaning efficiency in waste water treatment plants using two phenol-sensitive biosensors

Nistor, Catalin LU ; Rose, A; Farre, M; Stoica, Leonard LU ; Wollenberger, U; Ruzgas, Tautgirdas LU ; Pfeiffer, D; Barcelo, D; Gorton, Lo LU and Emnéus, Jenny LU (2002) In Analytica Chimica Acta 456(1). p.3-17
Abstract
Two amperometric biosensors based on the enzymes cellobiose dehydrogenase (CDH) and quinoprotein-dependent glucose dehydrogenase (GDH), have been applied for monitoring the phenolic content in water samples, collected at different stages of a waste water treatment process, thus representing different cleaning levels of two waste water treatment plants (WWTPs). The biosensor measurements were performed in-field, compared with the results obtained by liquid chromatography-mass spectrometry and were further correlated with the cleaning efficiencies of the WWTPs. The effect of several potentially interfering compounds on the sensor response was also studied. The general purpose of the study was to evaluate the potential use of biosensors, not... (More)
Two amperometric biosensors based on the enzymes cellobiose dehydrogenase (CDH) and quinoprotein-dependent glucose dehydrogenase (GDH), have been applied for monitoring the phenolic content in water samples, collected at different stages of a waste water treatment process, thus representing different cleaning levels of two waste water treatment plants (WWTPs). The biosensor measurements were performed in-field, compared with the results obtained by liquid chromatography-mass spectrometry and were further correlated with the cleaning efficiencies of the WWTPs. The effect of several potentially interfering compounds on the sensor response was also studied. The general purpose of the study was to evaluate the potential use of biosensors, not as quantitative tools for phenol analysis, but rather as screening tools indicating a certain trend, i.e. compounds present or not present, and potential correlation with sample toxicity. It was found that the biosensors and LC-MS results were not quantitatively comparable, however, both sensors could follow the decrease of the phenol content from the influent, primary treated and effluent waters. In addition, the correlation between biosensor inhibition and sample toxicity is discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
in-field analysis, toxicity, waste water, enzyme, biosensor
in
Analytica Chimica Acta
volume
456
issue
1
pages
3 - 17
publisher
Elsevier
external identifiers
  • wos:000174676000002
  • scopus:18344380253
ISSN
1873-4324
DOI
10.1016/S0003-2670(01)01015-7
language
English
LU publication?
yes
id
0ad65347-c693-4254-9540-bafc006a47cc (old id 341315)
date added to LUP
2007-11-02 13:31:16
date last changed
2017-10-01 04:42:58
@article{0ad65347-c693-4254-9540-bafc006a47cc,
  abstract     = {Two amperometric biosensors based on the enzymes cellobiose dehydrogenase (CDH) and quinoprotein-dependent glucose dehydrogenase (GDH), have been applied for monitoring the phenolic content in water samples, collected at different stages of a waste water treatment process, thus representing different cleaning levels of two waste water treatment plants (WWTPs). The biosensor measurements were performed in-field, compared with the results obtained by liquid chromatography-mass spectrometry and were further correlated with the cleaning efficiencies of the WWTPs. The effect of several potentially interfering compounds on the sensor response was also studied. The general purpose of the study was to evaluate the potential use of biosensors, not as quantitative tools for phenol analysis, but rather as screening tools indicating a certain trend, i.e. compounds present or not present, and potential correlation with sample toxicity. It was found that the biosensors and LC-MS results were not quantitatively comparable, however, both sensors could follow the decrease of the phenol content from the influent, primary treated and effluent waters. In addition, the correlation between biosensor inhibition and sample toxicity is discussed.},
  author       = {Nistor, Catalin and Rose, A and Farre, M and Stoica, Leonard and Wollenberger, U and Ruzgas, Tautgirdas and Pfeiffer, D and Barcelo, D and Gorton, Lo and Emnéus, Jenny},
  issn         = {1873-4324},
  keyword      = {in-field analysis,toxicity,waste water,enzyme,biosensor},
  language     = {eng},
  number       = {1},
  pages        = {3--17},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {In-field monitoring of cleaning efficiency in waste water treatment plants using two phenol-sensitive biosensors},
  url          = {http://dx.doi.org/10.1016/S0003-2670(01)01015-7},
  volume       = {456},
  year         = {2002},
}