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Effects of calcium deficiency and calcium supplementation on gastrectomy-induced osteopenia in the young male rat

Lehto-Axtelius, D; Surve, Vikas LU ; Johnell, Olof LU and Håkanson, Rolf LU (2002) In Scandinavian Journal of Gastroenterology 37(3). p.299-306
Abstract
Background: Surgical removal of the stomach (gastrectomy, Gx) induces osteopenia. In this study we compared the osteopenic effect of Gx with that induced by calcium (Ca) deficiency. Methods: Young male rats were subjected to Gx and/or low Ca diet (-Ca). A group of Gx rats received standard diet + oral Ca supplementation (+ Ca). The rats were killed at various times after the operation/start of treatment (longest time 12 weeks). After 8 weeks on low Ca diet, the blood Ca 2 concentration was lowered slightly in both Sham-operated and Gx rats. The calvariac were subjected to transillumination analysis and quantitative histomorphometry. Also the tibiae were subjected to histomorphometry. Results: Transillumination of the calvariae revealed... (More)
Background: Surgical removal of the stomach (gastrectomy, Gx) induces osteopenia. In this study we compared the osteopenic effect of Gx with that induced by calcium (Ca) deficiency. Methods: Young male rats were subjected to Gx and/or low Ca diet (-Ca). A group of Gx rats received standard diet + oral Ca supplementation (+ Ca). The rats were killed at various times after the operation/start of treatment (longest time 12 weeks). After 8 weeks on low Ca diet, the blood Ca 2 concentration was lowered slightly in both Sham-operated and Gx rats. The calvariac were subjected to transillumination analysis and quantitative histomorphometry. Also the tibiae were subjected to histomorphometry. Results: Transillumination of the calvariae revealed extensive bone loss in the rats that had been subjected to Gx and/or low Ca diet, Gx + Ca induced the same bone loss as Gx alone. These observations were later confirmed in quantitative terms by histomorphometry (Sham-Ca 56%, Gx 35%, Gx + Ca 32%, Gx - Ca 58% less bone area than in Sham). The osteopenia induced by Gx+ low Ca diet seemed more rapid in onset than that induced by Gx or low Ca diet alone. Tibiae from Gx rats and rats given a low Ca diet displayed a reduced trabecular bone volume (Sham-Ca 27%, remaining, Gx 36%, Gx + Ca 44%, Gx - Ca 1711) and reduced trabecular number (Sham-Ca 44% remaining, Gx 41%, Gx + Ca 56%, Gx - Ca 33%). The trabecular thickness was reduced in the Gx rats and Gx - Ca rats (Gx 78% remaining, Gx-Ca 63%) but not in Sham-operated rats receiving a low Ca-dict (95% remaining). Conclusion: Although the pattern of osteopenia was qualitatively quite similar in Gx rats and Ca-deficient rats, in quantitative terms the low Ca diet was more detrimental to bone than Gx. Ca deficiency induced a similar degree of osteopenia in both Sham and Gx rats. Ca supplementation failed to prevent the Gx-induced osteopenia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gastrectomy, calvaria, calcium supplementation, bone, calcium deficiency, osteopenia, rats, tibia
in
Scandinavian Journal of Gastroenterology
volume
37
issue
3
pages
299 - 306
publisher
Taylor & Francis
external identifiers
  • pmid:11916192
  • wos:000174212400008
  • scopus:0036188568
ISSN
1502-7708
DOI
10.1080/003655202317284200
language
English
LU publication?
yes
id
7a78f1f9-87ab-40ff-aa23-09a80356ccfe (old id 342409)
date added to LUP
2007-11-14 13:08:45
date last changed
2017-01-01 06:55:25
@article{7a78f1f9-87ab-40ff-aa23-09a80356ccfe,
  abstract     = {Background: Surgical removal of the stomach (gastrectomy, Gx) induces osteopenia. In this study we compared the osteopenic effect of Gx with that induced by calcium (Ca) deficiency. Methods: Young male rats were subjected to Gx and/or low Ca diet (-Ca). A group of Gx rats received standard diet + oral Ca supplementation (+ Ca). The rats were killed at various times after the operation/start of treatment (longest time 12 weeks). After 8 weeks on low Ca diet, the blood Ca 2 concentration was lowered slightly in both Sham-operated and Gx rats. The calvariac were subjected to transillumination analysis and quantitative histomorphometry. Also the tibiae were subjected to histomorphometry. Results: Transillumination of the calvariae revealed extensive bone loss in the rats that had been subjected to Gx and/or low Ca diet, Gx + Ca induced the same bone loss as Gx alone. These observations were later confirmed in quantitative terms by histomorphometry (Sham-Ca 56%, Gx 35%, Gx + Ca 32%, Gx - Ca 58% less bone area than in Sham). The osteopenia induced by Gx+ low Ca diet seemed more rapid in onset than that induced by Gx or low Ca diet alone. Tibiae from Gx rats and rats given a low Ca diet displayed a reduced trabecular bone volume (Sham-Ca 27%, remaining, Gx 36%, Gx + Ca 44%, Gx - Ca 1711) and reduced trabecular number (Sham-Ca 44% remaining, Gx 41%, Gx + Ca 56%, Gx - Ca 33%). The trabecular thickness was reduced in the Gx rats and Gx - Ca rats (Gx 78% remaining, Gx-Ca 63%) but not in Sham-operated rats receiving a low Ca-dict (95% remaining). Conclusion: Although the pattern of osteopenia was qualitatively quite similar in Gx rats and Ca-deficient rats, in quantitative terms the low Ca diet was more detrimental to bone than Gx. Ca deficiency induced a similar degree of osteopenia in both Sham and Gx rats. Ca supplementation failed to prevent the Gx-induced osteopenia.},
  author       = {Lehto-Axtelius, D and Surve, Vikas and Johnell, Olof and Håkanson, Rolf},
  issn         = {1502-7708},
  keyword      = {gastrectomy,calvaria,calcium supplementation,bone,calcium deficiency,osteopenia,rats,tibia},
  language     = {eng},
  number       = {3},
  pages        = {299--306},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Effects of calcium deficiency and calcium supplementation on gastrectomy-induced osteopenia in the young male rat},
  url          = {http://dx.doi.org/10.1080/003655202317284200},
  volume       = {37},
  year         = {2002},
}