Acoustophoresis enables the label-free separation of functionally different subsets of cultured bone marrow stromal cells
(2021) In Cytometry. Part A : the journal of the International Society for Analytical Cytology 99(5). p.476-487- Abstract
Culture-expanded mesenchymal stromal cells (MSCs) are promising candidates for clinical cell-based therapies. MSC products are heterogeneous and we therefore investigated whether acoustophoresis, an ultrasound-based separation technology, could be used for the label-free enrichment of functionally different MSC populations. Acoustophoresis uses an ultrasonic standing wave in a microchannel which differentially affects the movement of cells depending on their acoustophysical properties, such as size, density, and compressibility. Human bone marrow MSCs were generated by standard adherent culture in xenofree medium and separated by microchip acoustophoresis. MSCs with up to 20% higher proliferation and 1.7-fold increased clonogenic... (More)
Culture-expanded mesenchymal stromal cells (MSCs) are promising candidates for clinical cell-based therapies. MSC products are heterogeneous and we therefore investigated whether acoustophoresis, an ultrasound-based separation technology, could be used for the label-free enrichment of functionally different MSC populations. Acoustophoresis uses an ultrasonic standing wave in a microchannel which differentially affects the movement of cells depending on their acoustophysical properties, such as size, density, and compressibility. Human bone marrow MSCs were generated by standard adherent culture in xenofree medium and separated by microchip acoustophoresis. MSCs with up to 20% higher proliferation and 1.7-fold increased clonogenic potential were enriched in the side outlet of the chip compared to the input sample. These cells were significantly smaller (average diameter 14.5 ± 0.4 μm) compared to the center outlet fraction (average diameter 17.1 ± 0.6 μm) and expressed higher levels of genes related to proliferation and stem cell properties (i.e. Ki-67 (1.9-fold), Nanog1 (6.65-fold), Oct4 (2.9-fold), and CXCL12 (1.8-fold), n = 3) in the side outlet compared to input. Fractions of MSCs in G0 /G1 cell cycle phase were significantly enriched in the side fraction and an up to 2.8-fold increase of cells in S/G2 /M phases were observed in center fractions compared to side fractions and 1.3-fold increased compared to the input sample. Acoustophoresis did not compromise MSC phenotype, proliferation, clonogenic capacity, and viability (generally 87-98%), nor did it affect differentiation or immunomodulatory capacities. These results demonstrate that label-free acoustic separation can enrich functionally different MSC subsets which can potentially be employed to produce better-defined stromal cell products from cultured MSCs. Hence, acoustophoresis is a potentially promising separation technology to provide improved cell products for research and possible future clinical use.
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- author
- Olm, Franziska LU ; Lim, Hooi Ching LU ; Schallmoser, Katharina ; Strunk, Dirk ; Laurell, Thomas LU and Scheding, Stefan LU
- organization
-
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Bone marrow stem cells and cellular therapies (research group)
- Acoustofluidics group (research group)
- Division of Molecular Hematology (DMH)
- LUCC: Lund University Cancer Centre
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- NanoLund: Centre for Nanoscience
- Department of Biomedical Engineering
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- publishing date
- 2021-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cytometry. Part A : the journal of the International Society for Analytical Cytology
- volume
- 99
- issue
- 5
- pages
- 12 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85087302144
- pmid:32542988
- ISSN
- 1552-4930
- DOI
- 10.1002/cyto.a.24171
- language
- English
- LU publication?
- yes
- id
- 353a527c-b718-47df-856c-dee7ea80ac75
- date added to LUP
- 2020-06-19 09:30:13
- date last changed
- 2024-10-31 06:21:28
@article{353a527c-b718-47df-856c-dee7ea80ac75, abstract = {{<p>Culture-expanded mesenchymal stromal cells (MSCs) are promising candidates for clinical cell-based therapies. MSC products are heterogeneous and we therefore investigated whether acoustophoresis, an ultrasound-based separation technology, could be used for the label-free enrichment of functionally different MSC populations. Acoustophoresis uses an ultrasonic standing wave in a microchannel which differentially affects the movement of cells depending on their acoustophysical properties, such as size, density, and compressibility. Human bone marrow MSCs were generated by standard adherent culture in xenofree medium and separated by microchip acoustophoresis. MSCs with up to 20% higher proliferation and 1.7-fold increased clonogenic potential were enriched in the side outlet of the chip compared to the input sample. These cells were significantly smaller (average diameter 14.5 ± 0.4 μm) compared to the center outlet fraction (average diameter 17.1 ± 0.6 μm) and expressed higher levels of genes related to proliferation and stem cell properties (i.e. Ki-67 (1.9-fold), Nanog1 (6.65-fold), Oct4 (2.9-fold), and CXCL12 (1.8-fold), n = 3) in the side outlet compared to input. Fractions of MSCs in G0 /G1 cell cycle phase were significantly enriched in the side fraction and an up to 2.8-fold increase of cells in S/G2 /M phases were observed in center fractions compared to side fractions and 1.3-fold increased compared to the input sample. Acoustophoresis did not compromise MSC phenotype, proliferation, clonogenic capacity, and viability (generally 87-98%), nor did it affect differentiation or immunomodulatory capacities. These results demonstrate that label-free acoustic separation can enrich functionally different MSC subsets which can potentially be employed to produce better-defined stromal cell products from cultured MSCs. Hence, acoustophoresis is a potentially promising separation technology to provide improved cell products for research and possible future clinical use.</p>}}, author = {{Olm, Franziska and Lim, Hooi Ching and Schallmoser, Katharina and Strunk, Dirk and Laurell, Thomas and Scheding, Stefan}}, issn = {{1552-4930}}, language = {{eng}}, month = {{05}}, number = {{5}}, pages = {{476--487}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Cytometry. Part A : the journal of the International Society for Analytical Cytology}}, title = {{Acoustophoresis enables the label-free separation of functionally different subsets of cultured bone marrow stromal cells}}, url = {{http://dx.doi.org/10.1002/cyto.a.24171}}, doi = {{10.1002/cyto.a.24171}}, volume = {{99}}, year = {{2021}}, }