Utilising phase diagram to understand barley starch microsphere preparation in an aqueous two-phase system
Gidlöf, Zandra LU ; Lomstein Pedersen, Betty ; Nilsson, Lars LU ; Teleman, Anita ; Wahlgren, Marie LU
and Millqvist-Fureby, Anna
(2023)
In Colloids and Surfaces A: Physicochemical and Engineering Aspects
658.
- Abstract
In this work, a waxy barley starch-PEG aqueous two-phase system (ATPS) phase diagram was constructed, and starch microsphere preparation was explored at different phase diagram positions. The aim was to investigate starch-PEG ATPS phase behaviour and relate this to starch crystallisation and microsphere formation. The hypothesis was that phase diagram position would influence the starch microsphere preparation and the properties of the microspheres. The microsphere formation process was investigated with regard to microsphere development and starch crystallisation kinetics. Microsphere physicochemical properties and their development during different stages of the preparation were studied by examining freshly produced, freeze-dried, and... (More)
In this work, a waxy barley starch-PEG aqueous two-phase system (ATPS) phase diagram was constructed, and starch microsphere preparation was explored at different phase diagram positions. The aim was to investigate starch-PEG ATPS phase behaviour and relate this to starch crystallisation and microsphere formation. The hypothesis was that phase diagram position would influence the starch microsphere preparation and the properties of the microspheres. The microsphere formation process was investigated with regard to microsphere development and starch crystallisation kinetics. Microsphere physicochemical properties and their development during different stages of the preparation were studied by examining freshly produced, freeze-dried, and redispersed microspheres. Enzymatic hydrolysis of redispersed microspheres was also investigated. It was possible to produce microspheres from different positions in the phase diagram using 24 h incubation at 25 °C. However, the operational area for the used production conditions was relatively small compared to the biphasic region of the phase diagram. The main findings were that the starch-PEG ATPS phase behaviour can affect the rate of microsphere formation and particle size, but the additional properties of the dried and redispersed microspheres did not differ to a considerable extent. Thus, we have identified a robust production space where production parameters such as time to obtain microspheres can be considerably influenced by the ATPS system phase diagram position.
(Less)
- author
- Gidlöf, Zandra
LU
; Lomstein Pedersen, Betty
; Nilsson, Lars
LU
; Teleman, Anita
; Wahlgren, Marie
LU
and Millqvist-Fureby, Anna
- organization
- publishing date
- 2023-02-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aqueous two-phase system, ATPS, Barley starch, Microspheres, Phase diagram, Starch crystallisation
- in
- Colloids and Surfaces A: Physicochemical and Engineering Aspects
- volume
- 658
- article number
- 130652
- publisher
- Elsevier
- external identifiers
-
- scopus:85145556521
- ISSN
- 0927-7757
- DOI
- 10.1016/j.colsurfa.2022.130652
- language
- English
- LU publication?
- yes
- id
- 36a0ab9d-3aa1-41a7-ae4d-ce6baafba892
- date added to LUP
- 2023-02-09 15:02:49
- date last changed
- 2023-12-17 19:28:00
@article{36a0ab9d-3aa1-41a7-ae4d-ce6baafba892,
abstract = {{<p>In this work, a waxy barley starch-PEG aqueous two-phase system (ATPS) phase diagram was constructed, and starch microsphere preparation was explored at different phase diagram positions. The aim was to investigate starch-PEG ATPS phase behaviour and relate this to starch crystallisation and microsphere formation. The hypothesis was that phase diagram position would influence the starch microsphere preparation and the properties of the microspheres. The microsphere formation process was investigated with regard to microsphere development and starch crystallisation kinetics. Microsphere physicochemical properties and their development during different stages of the preparation were studied by examining freshly produced, freeze-dried, and redispersed microspheres. Enzymatic hydrolysis of redispersed microspheres was also investigated. It was possible to produce microspheres from different positions in the phase diagram using 24 h incubation at 25 °C. However, the operational area for the used production conditions was relatively small compared to the biphasic region of the phase diagram. The main findings were that the starch-PEG ATPS phase behaviour can affect the rate of microsphere formation and particle size, but the additional properties of the dried and redispersed microspheres did not differ to a considerable extent. Thus, we have identified a robust production space where production parameters such as time to obtain microspheres can be considerably influenced by the ATPS system phase diagram position.</p>}},
author = {{Gidlöf, Zandra and Lomstein Pedersen, Betty and Nilsson, Lars and Teleman, Anita and Wahlgren, Marie and Millqvist-Fureby, Anna}},
issn = {{0927-7757}},
keywords = {{Aqueous two-phase system; ATPS; Barley starch; Microspheres; Phase diagram; Starch crystallisation}},
language = {{eng}},
month = {{02}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces A: Physicochemical and Engineering Aspects}},
title = {{Utilising phase diagram to understand barley starch microsphere preparation in an aqueous two-phase system}},
url = {{http://dx.doi.org/10.1016/j.colsurfa.2022.130652}},
doi = {{10.1016/j.colsurfa.2022.130652}},
volume = {{658}},
year = {{2023}},
}