Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Proteins in deep eutectic solvents : Structure, dynamics and interactions with the solvent

Sanchez-Fernandez, Adrian LU orcid and Jackson, Andrew J. LU (2021) In Advances in Botanical Research 97.
Abstract

The structure and dynamics of proteins in solution are closely related to their activity and, ultimately, to their performance in products and technologies, for example, drug formulations and enzymatic catalysis. With this in mind, the study of the fundamental behavior of proteins in deep eutectic solvents not only provides insight into a novel alternative to aqueous and molecular organic solvents, but also contributes to understanding the potential behavior of these macromolecules in non-aqueous environments. In particular, it has relevance for systems with high levels of dehydration, liquid phases for dispersions at cryogenic temperatures, or systems with high ionic strength. In this chapter, we will explore current knowledge of the... (More)

The structure and dynamics of proteins in solution are closely related to their activity and, ultimately, to their performance in products and technologies, for example, drug formulations and enzymatic catalysis. With this in mind, the study of the fundamental behavior of proteins in deep eutectic solvents not only provides insight into a novel alternative to aqueous and molecular organic solvents, but also contributes to understanding the potential behavior of these macromolecules in non-aqueous environments. In particular, it has relevance for systems with high levels of dehydration, liquid phases for dispersions at cryogenic temperatures, or systems with high ionic strength. In this chapter, we will explore current knowledge of the physicochemical behavior of proteins in deep eutectic solvents. The local and global structure of proteins in these solvents will be presented and compared to those in aqueous solutions, organic solvents and ionic liquids. Furthermore, the characteristic behavior of proteins in deep eutectic solvents will be linked to solvation effects and the interaction of the macromolecules with the solvent, where specific electrostatic and hydrogen bond interactions can affect the structure and dynamics of proteins. Finally, challenges and opportunities in the field will be discussed, with the aim of guiding a common effort to develop a better understanding of the fundamental behavior of proteins in these interesting environments.

(Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Dynamics, Interactions, Protein incorporation, Structure
host publication
Advances in Botanical Research
series title
Advances in Botanical Research
volume
97
publisher
Academic Press
external identifiers
  • scopus:85092666197
ISSN
0065-2296
DOI
10.1016/bs.abr.2020.09.003
language
English
LU publication?
yes
id
37da358f-97fc-43b3-9b4c-9f8a4d5dda10
date added to LUP
2020-11-11 12:06:08
date last changed
2023-12-19 07:01:28
@inbook{37da358f-97fc-43b3-9b4c-9f8a4d5dda10,
  abstract     = {{<p>The structure and dynamics of proteins in solution are closely related to their activity and, ultimately, to their performance in products and technologies, for example, drug formulations and enzymatic catalysis. With this in mind, the study of the fundamental behavior of proteins in deep eutectic solvents not only provides insight into a novel alternative to aqueous and molecular organic solvents, but also contributes to understanding the potential behavior of these macromolecules in non-aqueous environments. In particular, it has relevance for systems with high levels of dehydration, liquid phases for dispersions at cryogenic temperatures, or systems with high ionic strength. In this chapter, we will explore current knowledge of the physicochemical behavior of proteins in deep eutectic solvents. The local and global structure of proteins in these solvents will be presented and compared to those in aqueous solutions, organic solvents and ionic liquids. Furthermore, the characteristic behavior of proteins in deep eutectic solvents will be linked to solvation effects and the interaction of the macromolecules with the solvent, where specific electrostatic and hydrogen bond interactions can affect the structure and dynamics of proteins. Finally, challenges and opportunities in the field will be discussed, with the aim of guiding a common effort to develop a better understanding of the fundamental behavior of proteins in these interesting environments.</p>}},
  author       = {{Sanchez-Fernandez, Adrian and Jackson, Andrew J.}},
  booktitle    = {{Advances in Botanical Research}},
  issn         = {{0065-2296}},
  keywords     = {{Dynamics; Interactions; Protein incorporation; Structure}},
  language     = {{eng}},
  publisher    = {{Academic Press}},
  series       = {{Advances in Botanical Research}},
  title        = {{Proteins in deep eutectic solvents : Structure, dynamics and interactions with the solvent}},
  url          = {{http://dx.doi.org/10.1016/bs.abr.2020.09.003}},
  doi          = {{10.1016/bs.abr.2020.09.003}},
  volume       = {{97}},
  year         = {{2021}},
}