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Monitoring of enzymatic hydrolysis of starch by microdialysis sampling coupled on-line to anion exchange chromatography and integrated pulsed electrochemical detection using post-column switching

Torto, N; Gorton, Lo LU ; Marko-Varga, György LU ; Emnéus, Jenny LU ; Akerberg, C; Zacchi, Guido LU and Laurell, Thomas LU (1997) In Biotechnology and Bioengineering 56(5). p.546-554
Abstract
A quantitative evaluation of the hydrolysis of wheat starch using Termamyl, a thermostable alpha-amylase (endo-l,4-alpha-D-glucan, glucanohydrolase; EC 3.2.1.78), is reported. Data from the monitoring of the hydrolysis of wheat starch indicated that, after 1 h, glucose and maltooligosaccharides up to DP 7 were the main hydrolysis products and thus enabled optimization of a liquefication step during the production of L-lactic acid. The monitoring system used, both in the on- and off-line mode, was based on continuous flow microdialysis sampling (CFMS) coupled to anion exchange chromatography and integrated pulsed electrochemical detection (IPED). A microdialysis probe equipped with a 5-mm polysulfone (SPS 4005) membrane, with a... (More)
A quantitative evaluation of the hydrolysis of wheat starch using Termamyl, a thermostable alpha-amylase (endo-l,4-alpha-D-glucan, glucanohydrolase; EC 3.2.1.78), is reported. Data from the monitoring of the hydrolysis of wheat starch indicated that, after 1 h, glucose and maltooligosaccharides up to DP 7 were the main hydrolysis products and thus enabled optimization of a liquefication step during the production of L-lactic acid. The monitoring system used, both in the on- and off-line mode, was based on continuous flow microdialysis sampling (CFMS) coupled to anion exchange chromatography and integrated pulsed electrochemical detection (IPED). A microdialysis probe equipped with a 5-mm polysulfone (SPS 4005) membrane, with a molecular-weight cut-off of 5 kDa, was used to sample the hydrolysis products of native wheat starch at 90 degrees C. Characteristic fingerprint separations were achieved by anion exchange chromatography after enzymatic hydrolysis. Post-column switching improved the detection and, consequently, also quantification of the hydrolysates as fouling of the electrode could be reduced. Maltooligosaccharide standards were used for quantification and to verify the elution of the hydrolysates by spiking the off-line samples. (C) 1997 John Wiley & Sons, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
post-column switching, microdialysis, monitoring, hydrolysates, integrated pulsed electrochemical detection
in
Biotechnology and Bioengineering
volume
56
issue
5
pages
546 - 554
publisher
John Wiley & Sons
external identifiers
  • wos:A1997YD66400008
  • pmid:18642275
  • scopus:0031555490
ISSN
1097-0290
DOI
10.1002/(SICI)1097-0290(19971205)56:5<546::AID-BIT8>3.0.CO;2-I
language
English
LU publication?
yes
id
a7deda23-5576-49f2-a58a-7e900e1471b5 (old id 3911464)
date added to LUP
2013-06-28 13:44:53
date last changed
2017-01-01 05:08:13
@article{a7deda23-5576-49f2-a58a-7e900e1471b5,
  abstract     = {A quantitative evaluation of the hydrolysis of wheat starch using Termamyl, a thermostable alpha-amylase (endo-l,4-alpha-D-glucan, glucanohydrolase; EC 3.2.1.78), is reported. Data from the monitoring of the hydrolysis of wheat starch indicated that, after 1 h, glucose and maltooligosaccharides up to DP 7 were the main hydrolysis products and thus enabled optimization of a liquefication step during the production of L-lactic acid. The monitoring system used, both in the on- and off-line mode, was based on continuous flow microdialysis sampling (CFMS) coupled to anion exchange chromatography and integrated pulsed electrochemical detection (IPED). A microdialysis probe equipped with a 5-mm polysulfone (SPS 4005) membrane, with a molecular-weight cut-off of 5 kDa, was used to sample the hydrolysis products of native wheat starch at 90 degrees C. Characteristic fingerprint separations were achieved by anion exchange chromatography after enzymatic hydrolysis. Post-column switching improved the detection and, consequently, also quantification of the hydrolysates as fouling of the electrode could be reduced. Maltooligosaccharide standards were used for quantification and to verify the elution of the hydrolysates by spiking the off-line samples. (C) 1997 John Wiley &amp; Sons, Inc.},
  author       = {Torto, N and Gorton, Lo and Marko-Varga, György and Emnéus, Jenny and Akerberg, C and Zacchi, Guido and Laurell, Thomas},
  issn         = {1097-0290},
  keyword      = {post-column switching,microdialysis,monitoring,hydrolysates,integrated pulsed electrochemical detection},
  language     = {eng},
  number       = {5},
  pages        = {546--554},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Monitoring of enzymatic hydrolysis of starch by microdialysis sampling coupled on-line to anion exchange chromatography and integrated pulsed electrochemical detection using post-column switching},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0290(19971205)56:5<546::AID-BIT8>3.0.CO;2-I},
  volume       = {56},
  year         = {1997},
}