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Exogenous pancreatic-like enzymes are recovered in the gut and improve growth of exocrine pancreatic insufficient pigs

Pierzynowski, Stefan LU ; Szwiec, K.; Piedra, J. L. Valverde; Gruijc, D.; Szymanczyk, S.; Swieboda, P.; Prykhodko, O.; Fedkiv, O.; Kruszewska, D. and Filip, R., et al. (2012) In Journal of Animal Science 90. p.324-326
Abstract
The exocrine pancreatic insufficient (EPI) pigs grow less due to different disturbances in feed digestion, absorption, and retention. Use of pancreatic-like enzymes of microbial origin in pigs may improve feed use and performance in slow-growing pigs. The aim was to study gut recovery and effectiveness of pancreatic-like enzymes of microbial origin supplementation on pig performance. Six male pigs 10 to 12 kg BW underwent pancreatic duct ligation surgery to induce total exocrine pancreatic insufficiency (EPI). Three cannulas to access the gastrointestinal tract content were installed in stomach, duodenum, and ileum in EPI pigs and in 3 control (healthy) pigs. One month after surgery, enzymes were given before feeding and digesta samples... (More)
The exocrine pancreatic insufficient (EPI) pigs grow less due to different disturbances in feed digestion, absorption, and retention. Use of pancreatic-like enzymes of microbial origin in pigs may improve feed use and performance in slow-growing pigs. The aim was to study gut recovery and effectiveness of pancreatic-like enzymes of microbial origin supplementation on pig performance. Six male pigs 10 to 12 kg BW underwent pancreatic duct ligation surgery to induce total exocrine pancreatic insufficiency (EPI). Three cannulas to access the gastrointestinal tract content were installed in stomach, duodenum, and ileum in EPI pigs and in 3 control (healthy) pigs. One month after surgery, enzymes were given before feeding and digesta samples were collected for analyses. The BW of EPI pigs did not increase during 1 mo following surgery (11.7 vs. 11.6 kg BW); however, BW increased after 1 wk of enzyme supplementation (12.1 kg BW). Coefficient of fat and N absorption increased (P < 0.05) in EPI pigs after enzyme supplementation. Activity of amylase, lipase, and protease in chyme samples of EPI pigs was very low compared to controls. In EPI pigs after enzyme supplementation, amylase activity increased from 5.32 to 72.9 units/mL but remained lower than that of healthy pigs (162.7 units/mL). Lipase activity increased from 79.1 to 421.6 units/mL, which was similar to that of controls (507.3 units/mL). Proteolytic activity increased from 7.8 to 69.7 units/mL but still did not reach control pigs (164.3 units/mL). In conclusion, exogenous microbial enzymes mimic endogenous pancreatic enzymes being recovered along the lumen of the gastrointestinal tract. These enzymes might be a useful tool to stimulate growth of slower-growing pigs after the weaning period. (Less)
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Contribution to journal
publication status
published
subject
keywords
exocrine pancreatic insufficiency, microbial enzyme
in
Journal of Animal Science
volume
90
pages
324 - 326
publisher
American Society of Animal Science
external identifiers
  • wos:000319673100104
  • pmid:23365368
  • scopus:84882720666
ISSN
1525-3163
DOI
10.2527/jas53872
language
English
LU publication?
yes
id
1c7a807f-d726-4914-aec0-5967462ef7df (old id 3931361)
date added to LUP
2013-07-15 14:51:44
date last changed
2017-03-05 03:20:20
@article{1c7a807f-d726-4914-aec0-5967462ef7df,
  abstract     = {The exocrine pancreatic insufficient (EPI) pigs grow less due to different disturbances in feed digestion, absorption, and retention. Use of pancreatic-like enzymes of microbial origin in pigs may improve feed use and performance in slow-growing pigs. The aim was to study gut recovery and effectiveness of pancreatic-like enzymes of microbial origin supplementation on pig performance. Six male pigs 10 to 12 kg BW underwent pancreatic duct ligation surgery to induce total exocrine pancreatic insufficiency (EPI). Three cannulas to access the gastrointestinal tract content were installed in stomach, duodenum, and ileum in EPI pigs and in 3 control (healthy) pigs. One month after surgery, enzymes were given before feeding and digesta samples were collected for analyses. The BW of EPI pigs did not increase during 1 mo following surgery (11.7 vs. 11.6 kg BW); however, BW increased after 1 wk of enzyme supplementation (12.1 kg BW). Coefficient of fat and N absorption increased (P &lt; 0.05) in EPI pigs after enzyme supplementation. Activity of amylase, lipase, and protease in chyme samples of EPI pigs was very low compared to controls. In EPI pigs after enzyme supplementation, amylase activity increased from 5.32 to 72.9 units/mL but remained lower than that of healthy pigs (162.7 units/mL). Lipase activity increased from 79.1 to 421.6 units/mL, which was similar to that of controls (507.3 units/mL). Proteolytic activity increased from 7.8 to 69.7 units/mL but still did not reach control pigs (164.3 units/mL). In conclusion, exogenous microbial enzymes mimic endogenous pancreatic enzymes being recovered along the lumen of the gastrointestinal tract. These enzymes might be a useful tool to stimulate growth of slower-growing pigs after the weaning period.},
  author       = {Pierzynowski, Stefan and Szwiec, K. and Piedra, J. L. Valverde and Gruijc, D. and Szymanczyk, S. and Swieboda, P. and Prykhodko, O. and Fedkiv, O. and Kruszewska, D. and Filip, R. and Botermans, J. and Svendsen, J. and Ushakova, G. and Kovalenko, T. and Osadchenko, I. and Goncharova, K. and Skibo, G. and Weström, Björn},
  issn         = {1525-3163},
  keyword      = {exocrine pancreatic insufficiency,microbial enzyme},
  language     = {eng},
  pages        = {324--326},
  publisher    = {American Society of Animal Science},
  series       = {Journal of Animal Science},
  title        = {Exogenous pancreatic-like enzymes are recovered in the gut and improve growth of exocrine pancreatic insufficient pigs},
  url          = {http://dx.doi.org/10.2527/jas53872},
  volume       = {90},
  year         = {2012},
}