Affinity partitioning of a Cellulomonas fimi beta-mannanase with a mannan-binding module in galactomannan/starch aqueous two-phase system
(2006) In Journal of Chromatography A 1123(1). p.53-59- Abstract
- A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achieved due to biospecificity of the mannan-binding module towards the top phase containing galactomannan. Experiments were conducted at pH 8 to prevent enzyme degradation of the phase containing galactomannan. Removal of the top phase polymer was accomplished by beta-mannanase degradation allowed by shifting to the optimal pH 6. In the combination... (More)
- A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achieved due to biospecificity of the mannan-binding module towards the top phase containing galactomannan. Experiments were conducted at pH 8 to prevent enzyme degradation of the phase containing galactomannan. Removal of the top phase polymer was accomplished by beta-mannanase degradation allowed by shifting to the optimal pH 6. In the combination with the genetic fusion of any given protein to the mannan-binding module, the results envision a general procedure for primary affinity recovery of such fusion proteins. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/399114
- author
- Antov, Mirjana ; Anderson, Lars LU ; Andersson, Alexandra LU ; Tjerneld, Folke LU and Stålbrand, Henrik LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- galactomannan, affinity-partitioning, aqueous two-phase system, beta-mannanase, mannan-binding module
- in
- Journal of Chromatography A
- volume
- 1123
- issue
- 1
- pages
- 53 - 59
- publisher
- Elsevier
- external identifiers
-
- wos:000239478100009
- pmid:16797561
- scopus:33745683948
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2006.05.021
- language
- English
- LU publication?
- yes
- id
- e9d7da90-3200-435e-b1fd-c5851525d53d (old id 399114)
- date added to LUP
- 2016-04-01 16:26:58
- date last changed
- 2022-03-15 00:34:32
@article{e9d7da90-3200-435e-b1fd-c5851525d53d,
abstract = {{A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achieved due to biospecificity of the mannan-binding module towards the top phase containing galactomannan. Experiments were conducted at pH 8 to prevent enzyme degradation of the phase containing galactomannan. Removal of the top phase polymer was accomplished by beta-mannanase degradation allowed by shifting to the optimal pH 6. In the combination with the genetic fusion of any given protein to the mannan-binding module, the results envision a general procedure for primary affinity recovery of such fusion proteins.}},
author = {{Antov, Mirjana and Anderson, Lars and Andersson, Alexandra and Tjerneld, Folke and Stålbrand, Henrik}},
issn = {{0021-9673}},
keywords = {{galactomannan; affinity-partitioning; aqueous two-phase system; beta-mannanase; mannan-binding module}},
language = {{eng}},
number = {{1}},
pages = {{53--59}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Affinity partitioning of a Cellulomonas fimi beta-mannanase with a mannan-binding module in galactomannan/starch aqueous two-phase system}},
url = {{http://dx.doi.org/10.1016/j.chroma.2006.05.021}},
doi = {{10.1016/j.chroma.2006.05.021}},
volume = {{1123}},
year = {{2006}},
}