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Affinity partitioning of a Cellulomonas fimi beta-mannanase with a mannan-binding module in galactomannan/starch aqueous two-phase system

Antov, Mirjana ; Anderson, Lars LU ; Andersson, Alexandra LU ; Tjerneld, Folke LU and Stålbrand, Henrik LU (2006) In Journal of Chromatography A 1123(1). p.53-59
Abstract
A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achieved due to biospecificity of the mannan-binding module towards the top phase containing galactomannan. Experiments were conducted at pH 8 to prevent enzyme degradation of the phase containing galactomannan. Removal of the top phase polymer was accomplished by beta-mannanase degradation allowed by shifting to the optimal pH 6. In the combination... (More)
A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achieved due to biospecificity of the mannan-binding module towards the top phase containing galactomannan. Experiments were conducted at pH 8 to prevent enzyme degradation of the phase containing galactomannan. Removal of the top phase polymer was accomplished by beta-mannanase degradation allowed by shifting to the optimal pH 6. In the combination with the genetic fusion of any given protein to the mannan-binding module, the results envision a general procedure for primary affinity recovery of such fusion proteins. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
galactomannan, affinity-partitioning, aqueous two-phase system, beta-mannanase, mannan-binding module
in
Journal of Chromatography A
volume
1123
issue
1
pages
53 - 59
publisher
Elsevier
external identifiers
  • wos:000239478100009
  • pmid:16797561
  • scopus:33745683948
ISSN
0021-9673
DOI
10.1016/j.chroma.2006.05.021
language
English
LU publication?
yes
id
e9d7da90-3200-435e-b1fd-c5851525d53d (old id 399114)
date added to LUP
2016-04-01 16:26:58
date last changed
2021-10-06 01:48:00
@article{e9d7da90-3200-435e-b1fd-c5851525d53d,
  abstract     = {A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achieved due to biospecificity of the mannan-binding module towards the top phase containing galactomannan. Experiments were conducted at pH 8 to prevent enzyme degradation of the phase containing galactomannan. Removal of the top phase polymer was accomplished by beta-mannanase degradation allowed by shifting to the optimal pH 6. In the combination with the genetic fusion of any given protein to the mannan-binding module, the results envision a general procedure for primary affinity recovery of such fusion proteins.},
  author       = {Antov, Mirjana and Anderson, Lars and Andersson, Alexandra and Tjerneld, Folke and Stålbrand, Henrik},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1},
  pages        = {53--59},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Affinity partitioning of a Cellulomonas fimi beta-mannanase with a mannan-binding module in galactomannan/starch aqueous two-phase system},
  url          = {http://dx.doi.org/10.1016/j.chroma.2006.05.021},
  doi          = {10.1016/j.chroma.2006.05.021},
  volume       = {1123},
  year         = {2006},
}