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Housekeeping genes for phylogenetic analysis of eutherian relationships

Kullberg, Morgan LU ; Nilsson, Maria LU ; Arnason, Ulfur LU ; Harley, Eric H. and Janke, Axel LU (2006) In Molecular biology and evolution 23(8). p.1493-1503
Abstract
The molecular relationship of placental mammals has attracted great interest in recent years. However, 2 crucial and conflicting hypotheses remain, one with respect to the position of the root of the eutherian tree and the other the relationship between the orders Rodentia, Lagomorpha (rabbits, hares), and Primates. Although most mitochondrial (mt) analyses have suggested that rodents have a basal position in the eutherian tree, some nuclear data in combination with mt-rRNA genes have placed the root on the so-called African clade or on a branch that includes this clade and the Xenarthra (e.g., anteater and armadillo). In order to generate a new and independent set of molecular data for phylogenetic analysis, we have established cDNA... (More)
The molecular relationship of placental mammals has attracted great interest in recent years. However, 2 crucial and conflicting hypotheses remain, one with respect to the position of the root of the eutherian tree and the other the relationship between the orders Rodentia, Lagomorpha (rabbits, hares), and Primates. Although most mitochondrial (mt) analyses have suggested that rodents have a basal position in the eutherian tree, some nuclear data in combination with mt-rRNA genes have placed the root on the so-called African clade or on a branch that includes this clade and the Xenarthra (e.g., anteater and armadillo). In order to generate a new and independent set of molecular data for phylogenetic analysis, we have established cDNA sequences from different tissues of various mammalian species. With this in mind, we have identified and sequenced 8 housekeeping genes with moderately fast rate of evolution from 22 placental mammals, representing I I orders. In order to determine the root of the eutherian tree, the same genes were also sequenced for 3 marsupial species, which were used as outgroup. Inconsistent with the analyses of nuclear + mt-rRNA gene data, the current data set did not favor a basal position of the African clade or Xenarthra in the eutherian tree. Similarly, by joining rodents and lagomorphs on the same basal branch (Glires hypothesis), the data set is also inconsistent with the tree commonly favored in mtDNA analyses. The analyses of the currently established sequences have helped examination of problematic parts in the eutherian tree at the same time as they caution against suggestions that have claimed that basal eutherian relationships have been conclusively settled. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cDNA, rooting, outgroup, phylogeny, mammals
in
Molecular biology and evolution
volume
23
issue
8
pages
1493 - 1503
publisher
Oxford University Press
external identifiers
  • wos:000239281200005
  • scopus:33748068074
ISSN
0737-4038
DOI
10.1093/molbev/msl027
language
English
LU publication?
yes
id
3637150a-14a2-4c87-b1a6-63970c9b6921 (old id 399395)
date added to LUP
2016-04-01 12:14:36
date last changed
2021-08-18 05:27:43
@article{3637150a-14a2-4c87-b1a6-63970c9b6921,
  abstract     = {The molecular relationship of placental mammals has attracted great interest in recent years. However, 2 crucial and conflicting hypotheses remain, one with respect to the position of the root of the eutherian tree and the other the relationship between the orders Rodentia, Lagomorpha (rabbits, hares), and Primates. Although most mitochondrial (mt) analyses have suggested that rodents have a basal position in the eutherian tree, some nuclear data in combination with mt-rRNA genes have placed the root on the so-called African clade or on a branch that includes this clade and the Xenarthra (e.g., anteater and armadillo). In order to generate a new and independent set of molecular data for phylogenetic analysis, we have established cDNA sequences from different tissues of various mammalian species. With this in mind, we have identified and sequenced 8 housekeeping genes with moderately fast rate of evolution from 22 placental mammals, representing I I orders. In order to determine the root of the eutherian tree, the same genes were also sequenced for 3 marsupial species, which were used as outgroup. Inconsistent with the analyses of nuclear + mt-rRNA gene data, the current data set did not favor a basal position of the African clade or Xenarthra in the eutherian tree. Similarly, by joining rodents and lagomorphs on the same basal branch (Glires hypothesis), the data set is also inconsistent with the tree commonly favored in mtDNA analyses. The analyses of the currently established sequences have helped examination of problematic parts in the eutherian tree at the same time as they caution against suggestions that have claimed that basal eutherian relationships have been conclusively settled.},
  author       = {Kullberg, Morgan and Nilsson, Maria and Arnason, Ulfur and Harley, Eric H. and Janke, Axel},
  issn         = {0737-4038},
  language     = {eng},
  number       = {8},
  pages        = {1493--1503},
  publisher    = {Oxford University Press},
  series       = {Molecular biology and evolution},
  title        = {Housekeeping genes for phylogenetic analysis of eutherian relationships},
  url          = {http://dx.doi.org/10.1093/molbev/msl027},
  doi          = {10.1093/molbev/msl027},
  volume       = {23},
  year         = {2006},
}