Cyclodextrin-surfactant complex: A new route in DNA decompaction
(2008) In Biomacromolecules 9(3). p.772-775- Abstract
- In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and... (More)
- In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and biological interfaces is small. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1191576
- author
- Gonzalez Perez, Alfredo LU ; Dias, Rita LU ; Nylander, Tommy LU and Lindman, Björn LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomacromolecules
- volume
- 9
- issue
- 3
- pages
- 772 - 775
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000253926400003
- scopus:41949138209
- pmid:18257531
- ISSN
- 1526-4602
- DOI
- 10.1021/bm7012907
- language
- English
- LU publication?
- yes
- id
- 3ac813a2-161d-435e-8561-76583edf4d4e (old id 1191576)
- date added to LUP
- 2016-04-01 12:37:37
- date last changed
- 2022-01-27 07:39:27
@article{3ac813a2-161d-435e-8561-76583edf4d4e, abstract = {{In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and biological interfaces is small.}}, author = {{Gonzalez Perez, Alfredo and Dias, Rita and Nylander, Tommy and Lindman, Björn}}, issn = {{1526-4602}}, language = {{eng}}, number = {{3}}, pages = {{772--775}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Biomacromolecules}}, title = {{Cyclodextrin-surfactant complex: A new route in DNA decompaction}}, url = {{http://dx.doi.org/10.1021/bm7012907}}, doi = {{10.1021/bm7012907}}, volume = {{9}}, year = {{2008}}, }