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Utility of G protein-coupled receptor 35 expression for predicting outcome in colon cancer

Ali, Haytham; AbdelMageed, Manar; Olsson, Lina LU ; Israelsson, Anne; Lindmark, Gudrun LU ; Hammarström, Marie Louise; Hammarström, Sten and Sitohy, Basel (2019) In Tumor Biology 41(6). p.1-11
Abstract

The utility of mRNA and protein determinations of G protein-coupled receptor 35, that is, GPR35a (GPR35 V1) and GPR35b (GPR35 V2/3), as indicators of outcome for colon cancer patients after curative surgery was investigated. Expression levels of V1 and V2/3 GPR35, carcinoembryonic antigen and CXCL17 mRNAs were assessed in primary tumours and regional lymph nodes of 121 colon cancer patients (stage I–IV), colon cancer cell lines and control colon epithelial cells using real-time quantitative reverse transcriptase-polymerase chain reaction. Expression of G protein-coupled receptor 35 was investigated by two-colour immunohistochemistry and immunomorphometry. GPR35 V2/3 mRNA, but not V1 mRNA, was expressed in colon cancer cell lines,... (More)

The utility of mRNA and protein determinations of G protein-coupled receptor 35, that is, GPR35a (GPR35 V1) and GPR35b (GPR35 V2/3), as indicators of outcome for colon cancer patients after curative surgery was investigated. Expression levels of V1 and V2/3 GPR35, carcinoembryonic antigen and CXCL17 mRNAs were assessed in primary tumours and regional lymph nodes of 121 colon cancer patients (stage I–IV), colon cancer cell lines and control colon epithelial cells using real-time quantitative reverse transcriptase-polymerase chain reaction. Expression of G protein-coupled receptor 35 was investigated by two-colour immunohistochemistry and immunomorphometry. GPR35 V2/3 mRNA, but not V1 mRNA, was expressed in colon cancer cell lines, primary colon tumours and control colon epithelial cells. Haematoxylin and eosin positive (H&E(+)), but not H&E(–), lymph nodes expressed high levels of GPR35 V2/3 mRNA (P<0.0001). GPR35b and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer tumour cells. Kaplan–Meier and hazard ratio analysis revealed that patients with lymph nodes expressing high levels of GPR35 V2/3 mRNA and, in particular, in the group of patients with lymph nodes also expressing carcinoembryonic antigen mRNA, had a short disease-free survival time, 67 months versus 122 months at 12-year follow-up (difference: 55 months, P = 0.001; hazard ratio: 3.6, P = 0.002). In conclusion, high level expression of G protein-coupled receptor 35 V2/3 mRNA in regional lymph nodes of colon cancer patients is a sign of poor prognosis.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
CEA, colon cancer, CXCL17 mRNA, GPR35 V1 mRNA, GPR35 V2/3 mRNA, GPR35b, immunohistochemistry, qRT-PCR
in
Tumor Biology
volume
41
issue
6
pages
1 - 11
publisher
Springer
external identifiers
  • scopus:85068211995
ISSN
1010-4283
DOI
10.1177/1010428319858885
language
English
LU publication?
yes
id
3b8afa0a-0f04-49b1-810c-a44ddf0fea40
date added to LUP
2019-07-09 16:52:25
date last changed
2019-08-06 03:24:00
@article{3b8afa0a-0f04-49b1-810c-a44ddf0fea40,
  abstract     = {<p>The utility of mRNA and protein determinations of G protein-coupled receptor 35, that is, GPR35a (GPR35 V1) and GPR35b (GPR35 V2/3), as indicators of outcome for colon cancer patients after curative surgery was investigated. Expression levels of V1 and V2/3 GPR35, carcinoembryonic antigen and CXCL17 mRNAs were assessed in primary tumours and regional lymph nodes of 121 colon cancer patients (stage I–IV), colon cancer cell lines and control colon epithelial cells using real-time quantitative reverse transcriptase-polymerase chain reaction. Expression of G protein-coupled receptor 35 was investigated by two-colour immunohistochemistry and immunomorphometry. GPR35 V2/3 mRNA, but not V1 mRNA, was expressed in colon cancer cell lines, primary colon tumours and control colon epithelial cells. Haematoxylin and eosin positive (H&amp;E(+)), but not H&amp;E(–), lymph nodes expressed high levels of GPR35 V2/3 mRNA (P&lt;0.0001). GPR35b and carcinoembryonic antigen proteins were simultaneously expressed in many colon cancer tumour cells. Kaplan–Meier and hazard ratio analysis revealed that patients with lymph nodes expressing high levels of GPR35 V2/3 mRNA and, in particular, in the group of patients with lymph nodes also expressing carcinoembryonic antigen mRNA, had a short disease-free survival time, 67 months versus 122 months at 12-year follow-up (difference: 55 months, P = 0.001; hazard ratio: 3.6, P = 0.002). In conclusion, high level expression of G protein-coupled receptor 35 V2/3 mRNA in regional lymph nodes of colon cancer patients is a sign of poor prognosis.</p>},
  author       = {Ali, Haytham and AbdelMageed, Manar and Olsson, Lina and Israelsson, Anne and Lindmark, Gudrun and Hammarström, Marie Louise and Hammarström, Sten and Sitohy, Basel},
  issn         = {1010-4283},
  keyword      = {CEA,colon cancer,CXCL17 mRNA,GPR35 V1 mRNA,GPR35 V2/3 mRNA,GPR35b,immunohistochemistry,qRT-PCR},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {1--11},
  publisher    = {Springer},
  series       = {Tumor Biology},
  title        = {Utility of G protein-coupled receptor 35 expression for predicting outcome in colon cancer},
  url          = {http://dx.doi.org/10.1177/1010428319858885},
  volume       = {41},
  year         = {2019},
}