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Indoor PM2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM2.5

Wierzbicka, Aneta LU orcid ; Omelekhina, Yuliya LU ; Saber, Anne Thoustrup ; Bloom, Erica LU ; Gren, Louise LU ; Poulsen, Sarah Søs ; Strandberg, Bo LU ; Pagels, Joakim LU and Jacobsen, Nicklas Raun (2022) In Indoor Air 32(12).
Abstract

We spend most of our time indoors; however, little is known about the effects of exposure to aerosol particles indoors. We aimed to determine differences in relative toxicity and physicochemical properties of PM
2.5 collected simultaneously indoors (PM
2.5 INDOOR ) and outdoors (PM
2.5 OUTDOOR ) in 15 occupied homes in southern Sweden. Collected particles were extracted from filters, pooled (indoor and outdoor separately), and characterized for chemical composition and endotoxins before being tested for toxicity in mice via intratracheal instillation. Various endpoints including lung inflammation, genotoxicity, and acute-phase response in lung and liver were assessed 1, 3, and 28 days post-exposure. Chemical composition... (More)

We spend most of our time indoors; however, little is known about the effects of exposure to aerosol particles indoors. We aimed to determine differences in relative toxicity and physicochemical properties of PM
2.5 collected simultaneously indoors (PM
2.5 INDOOR ) and outdoors (PM
2.5 OUTDOOR ) in 15 occupied homes in southern Sweden. Collected particles were extracted from filters, pooled (indoor and outdoor separately), and characterized for chemical composition and endotoxins before being tested for toxicity in mice via intratracheal instillation. Various endpoints including lung inflammation, genotoxicity, and acute-phase response in lung and liver were assessed 1, 3, and 28 days post-exposure. Chemical composition of particles used in toxicological assessment was compared to particles analyzed without extraction. Time-resolved particle mass and number concentrations were monitored. PM
2.5 INDOOR showed higher relative concentrations (μg mg
-1 ) of metals, PAHs, and endotoxins compared to PM
2.5 OUTDOOR . These differences may be linked to PM
2.5 INDOOR causing significantly higher lung inflammation and lung acute-phase response 1 day post-exposure compared to PM
2.5 OUTDOOR and vehicle controls, respectively. None of the tested materials caused genotoxicity. PM
2.5 INDOOR displayed higher relative toxicity than PM
2.5 OUTDOOR under the studied conditions, that is, wintertime with reduced air exchange rates, high influence of indoor sources, and relatively low outdoor concentrations of PM. Reducing PM
2.5 INDOOR exposure requires reduction of both infiltration from outdoors and indoor-generated particles.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Mice, Air Pollutants/analysis, Air Pollution, Indoor/adverse effects, Environmental Monitoring, Particle Size, Acute-Phase Reaction, Sweden, Particulate Matter/analysis, Pneumonia/etiology, aerosol, home, indoor/outdoor ratio, physicochemical characteristics, PM2.5, real-life exposure, toxicity
in
Indoor Air
volume
32
issue
12
article number
e13177
pages
15 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:36567521
  • scopus:85145061636
ISSN
0905-6947
DOI
10.1111/ina.13177
project
Airborne particles in our homes: cocktail effects, chemical composition, physical characteristics and toxicity
language
English
LU publication?
yes
additional info
© 2022 The Authors. Indoor Air published by John Wiley & Sons Ltd.
id
3c94b026-0bfd-4a71-82d1-d5210ffdf36b
date added to LUP
2023-01-13 14:22:08
date last changed
2024-04-04 15:52:30
@article{3c94b026-0bfd-4a71-82d1-d5210ffdf36b,
  abstract     = {{<p>We spend most of our time indoors; however, little is known about the effects of exposure to aerosol particles indoors. We aimed to determine differences in relative toxicity and physicochemical properties of PM<br>
 2.5 collected simultaneously indoors (PM<br>
 2.5 INDOOR ) and outdoors (PM<br>
 2.5 OUTDOOR ) in 15 occupied homes in southern Sweden. Collected particles were extracted from filters, pooled (indoor and outdoor separately), and characterized for chemical composition and endotoxins before being tested for toxicity in mice via intratracheal instillation. Various endpoints including lung inflammation, genotoxicity, and acute-phase response in lung and liver were assessed 1, 3, and 28 days post-exposure. Chemical composition of particles used in toxicological assessment was compared to particles analyzed without extraction. Time-resolved particle mass and number concentrations were monitored. PM <br>
 2.5 INDOOR showed higher relative concentrations (μg mg<br>
 -1 ) of metals, PAHs, and endotoxins compared to PM<br>
 2.5 OUTDOOR . These differences may be linked to PM<br>
 2.5 INDOOR causing significantly higher lung inflammation and lung acute-phase response 1 day post-exposure compared to PM<br>
 2.5 OUTDOOR and vehicle controls, respectively. None of the tested materials caused genotoxicity. PM<br>
 2.5 INDOOR displayed higher relative toxicity than PM<br>
 2.5 OUTDOOR under the studied conditions, that is, wintertime with reduced air exchange rates, high influence of indoor sources, and relatively low outdoor concentrations of PM. Reducing PM <br>
 2.5 INDOOR exposure requires reduction of both infiltration from outdoors and indoor-generated particles.<br>
 </p>}},
  author       = {{Wierzbicka, Aneta and Omelekhina, Yuliya and Saber, Anne Thoustrup and Bloom, Erica and Gren, Louise and Poulsen, Sarah Søs and Strandberg, Bo and Pagels, Joakim and Jacobsen, Nicklas Raun}},
  issn         = {{0905-6947}},
  keywords     = {{Animals; Mice; Air Pollutants/analysis; Air Pollution, Indoor/adverse effects; Environmental Monitoring; Particle Size; Acute-Phase Reaction; Sweden; Particulate Matter/analysis; Pneumonia/etiology; aerosol, home, indoor/outdoor ratio, physicochemical characteristics, PM2.5, real-life exposure, toxicity}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Indoor Air}},
  title        = {{Indoor PM2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM2.5}},
  url          = {{http://dx.doi.org/10.1111/ina.13177}},
  doi          = {{10.1111/ina.13177}},
  volume       = {{32}},
  year         = {{2022}},
}