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Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Wallman, Lars LU ; Ekström, Simon LU ; Magnusson, Mattias LU ; Bolmsjö, Gunnar LU ; Olsson, Magnus LU ; Nilsson, Johan LU ; Marko-Varga, György LU and Laurell, Thomas LU (2006) In Measurement Science & Technology 17(12). p.3147-3153
Abstract
A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
piezodispensing, capillary force, microfluidics, MALDI, mass spectrometry, microchip, robotic, MALDI-TOF MS, automation, protein analysis, proteomics, solid phase extraction
in
Measurement Science & Technology
volume
17
issue
12
pages
3147 - 3153
publisher
IOP Publishing
external identifiers
  • wos:000242528500008
  • scopus:33846110953
ISSN
0957-0233
DOI
10.1088/0957-0233/17/12/S05
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Robotics (ceased) (LUR000026), Analytical Chemistry (S/LTH) (011001004), Departments at LTH (011200000)
id
3cabce1b-3899-494c-99fd-26300e7488be (old id 748440)
date added to LUP
2016-04-04 09:38:36
date last changed
2025-10-14 11:16:19
@article{3cabce1b-3899-494c-99fd-26300e7488be,
  abstract     = {{A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.}},
  author       = {{Wallman, Lars and Ekström, Simon and Magnusson, Mattias and Bolmsjö, Gunnar and Olsson, Magnus and Nilsson, Johan and Marko-Varga, György and Laurell, Thomas}},
  issn         = {{0957-0233}},
  keywords     = {{piezodispensing; capillary force; microfluidics; MALDI; mass spectrometry; microchip; robotic; MALDI-TOF MS; automation; protein analysis; proteomics; solid phase extraction}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{3147--3153}},
  publisher    = {{IOP Publishing}},
  series       = {{Measurement Science & Technology}},
  title        = {{Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout}},
  url          = {{http://dx.doi.org/10.1088/0957-0233/17/12/S05}},
  doi          = {{10.1088/0957-0233/17/12/S05}},
  volume       = {{17}},
  year         = {{2006}},
}