Flow cytometric analysis reveals culture condition dependent variations in phenotypic heterogeneity of Limosilactobacillus reuteri
(2021) In Scientific Reports 11.- Abstract
- Optimisation of cultivation conditions in the industrial production of probiotics is crucial to reach a high-quality product with retained probiotic functionality. Flow cytometry-based descriptors of bacterial morphology may be used as markers to estimate physiological fitness during cultivation, and can be applied for online monitoring to avoid suboptimal growth. In the current study, the effects of temperature, initial pH and oxygen levels on cell growth and cell size distributions of Limosilactobacillus reuteri DSM 17938 were measured using multivariate flow cytometry. A pleomorphic behaviour was evident from the measurements of light scatter and pulse width distributions. A pattern of high growth yielding smaller cells and less... (More)
- Optimisation of cultivation conditions in the industrial production of probiotics is crucial to reach a high-quality product with retained probiotic functionality. Flow cytometry-based descriptors of bacterial morphology may be used as markers to estimate physiological fitness during cultivation, and can be applied for online monitoring to avoid suboptimal growth. In the current study, the effects of temperature, initial pH and oxygen levels on cell growth and cell size distributions of Limosilactobacillus reuteri DSM 17938 were measured using multivariate flow cytometry. A pleomorphic behaviour was evident from the measurements of light scatter and pulse width distributions. A pattern of high growth yielding smaller cells and less heterogeneous populations could be observed. Analysis of pulse width distributions revealed significant morphological heterogeneities within the bacterial cell population under non-optimal growth conditions, and pointed towards low temperature, high initial pH, and high oxygen levels all being triggers for changes in morphology towards cell chain formation. However, cell size did not correlate to survivability after freeze-thaw or freeze-drying stress, indicating that it is not a key determinant for physical stress tolerance. The fact that L. reuteri morphology varies depending on cultivation conditions suggests that it can be used as marker for estimating physiological fitness and responses to its environment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3f1f19bd-d43b-4b09-ae41-6ba1e429df63
- author
- Seshagiri Rao, Nikhil LU ; Lundberg, Ludwig ; Palmkron, Shuai LU ; Håkansson, Sebastian LU ; Bergenståhl, Björn LU and Carlquist, Magnus LU
- organization
- publishing date
- 2021-12-07
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 11
- article number
- 23567 (2021)
- pages
- 12 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85120907568
- pmid:34876641
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-021-02919-3
- language
- English
- LU publication?
- yes
- id
- 3f1f19bd-d43b-4b09-ae41-6ba1e429df63
- date added to LUP
- 2021-12-27 23:52:44
- date last changed
- 2024-01-08 15:53:51
@article{3f1f19bd-d43b-4b09-ae41-6ba1e429df63,
abstract = {{Optimisation of cultivation conditions in the industrial production of probiotics is crucial to reach a high-quality product with retained probiotic functionality. Flow cytometry-based descriptors of bacterial morphology may be used as markers to estimate physiological fitness during cultivation, and can be applied for online monitoring to avoid suboptimal growth. In the current study, the effects of temperature, initial pH and oxygen levels on cell growth and cell size distributions of <i>Limosilactobacillus reuteri</i> DSM 17938 were measured using multivariate flow cytometry. A pleomorphic behaviour was evident from the measurements of light scatter and pulse width distributions. A pattern of high growth yielding smaller cells and less heterogeneous populations could be observed. Analysis of pulse width distributions revealed significant morphological heterogeneities within the bacterial cell population under non-optimal growth conditions, and pointed towards low temperature, high initial pH, and high oxygen levels all being triggers for changes in morphology towards cell chain formation. However, cell size did not correlate to survivability after freeze-thaw or freeze-drying stress, indicating that it is not a key determinant for physical stress tolerance. The fact that <i>L. reuteri</i> morphology varies depending on cultivation conditions suggests that it can be used as marker for estimating physiological fitness and responses to its environment.}},
author = {{Seshagiri Rao, Nikhil and Lundberg, Ludwig and Palmkron, Shuai and Håkansson, Sebastian and Bergenståhl, Björn and Carlquist, Magnus}},
issn = {{2045-2322}},
language = {{eng}},
month = {{12}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Flow cytometric analysis reveals culture condition dependent variations in phenotypic heterogeneity of <i>Limosilactobacillus reuteri</i>}},
url = {{http://dx.doi.org/10.1038/s41598-021-02919-3}},
doi = {{10.1038/s41598-021-02919-3}},
volume = {{11}},
year = {{2021}},
}