Enhancing the stability of probiotics: Freeze-drying and encapsulation
(2024) 1.- Abstract
- This research dives into the intricate world of freeze-drying, an essential process in pharmaceutical and probiotic industries, to better understand the factors affecting probiotic stability during storage. The process of freeze-drying and the composition of freeze-dried products significantly affect the stability of probiotics, yet factors such as the material’s encapsulation properties remain relatively unexplored, demanding further research.
This thesis will explore the effects of lyoprotectants, freezing, annealing, and drying on the structure of freeze-dried products. It aims to provide a comprehensive understanding of how varying conditions influence structural characteristics and their correlation with the storage stability of... (More) - This research dives into the intricate world of freeze-drying, an essential process in pharmaceutical and probiotic industries, to better understand the factors affecting probiotic stability during storage. The process of freeze-drying and the composition of freeze-dried products significantly affect the stability of probiotics, yet factors such as the material’s encapsulation properties remain relatively unexplored, demanding further research.
This thesis will explore the effects of lyoprotectants, freezing, annealing, and drying on the structure of freeze-dried products. It aims to provide a comprehensive understanding of how varying conditions influence structural characteristics and their correlation with the storage stability of probiotics. We will also explore the key differences between vial and pellet formulations, and discuss topics critical for ensuring stability during storage such as glass transition temperatures.
The investigation uses techniques such as scanning electron microscopy (SEM) and X-ray micro- computed tomography (μCT) to investigate the structural attributes of freeze-dried material. The thickness of the encapsulating material is the central focus, revealing its crucial role in protecting the probiotic cells. The findings indicates that a notably thicker material provides increased protection, enhancing probiotic storage stability. (Less) - Abstract (Swedish)
- Denna forskning fördjupar sig i frystorkning, en essentiell process inom läkemedels- och probiotikaindustrin, för att bättre förstå faktorer som påverkar probiotisk stabilitet under lagring. Frystorkningsprocessen och sammansättningen av frystorkade produkter påverkar signifikant probiotikas stabilitet, men faktorer som materialens inkapslingsegenskaper förblir relativt outforskade och kräver ytterligare forskning.
Denna avhandling kommer att utforska effekterna av lyoprotektanter, frysning, temperering och torkning på strukturen av frystorkade produkter. Målet är att ge en omfattande förståelse för hur varierande förhållanden påverkar strukturella egenskaper och deras korrelation med probiotikas lagringsstabilitet. Vi kommer även att... (More) - Denna forskning fördjupar sig i frystorkning, en essentiell process inom läkemedels- och probiotikaindustrin, för att bättre förstå faktorer som påverkar probiotisk stabilitet under lagring. Frystorkningsprocessen och sammansättningen av frystorkade produkter påverkar signifikant probiotikas stabilitet, men faktorer som materialens inkapslingsegenskaper förblir relativt outforskade och kräver ytterligare forskning.
Denna avhandling kommer att utforska effekterna av lyoprotektanter, frysning, temperering och torkning på strukturen av frystorkade produkter. Målet är att ge en omfattande förståelse för hur varierande förhållanden påverkar strukturella egenskaper och deras korrelation med probiotikas lagringsstabilitet. Vi kommer även att undersöka de viktigaste skillnaderna mellan formuleringar i vialer och pellets, samt diskutera ämnen som är kritiska för att säkerställa stabilitet under lagring, såsom glastransitionstemperaturer.
Undersökningen använder tekniker som skanningselektronmikroskopi (SEM) och röntgenmikro-datortomografi (μCT) för att undersöka strukturella egenskaper hos frystorkat material. Fokus ligger på tjockleken hos inkapslande material, vilket avslöjar dess avgörande roll i att skydda probiotikaceller. Resultaten indikerar att ett märkbart tjockare material ger ökad skyddsnivå och förbättrar probiotikas lagringsstabilitet.
(Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/273021c0-1867-48d8-88b5-967a5c120c49
- author
- Bai, Shuai LU
- supervisor
-
- Marie Wahlgren LU
- Björn Bergenståhl LU
- Anna Fureby LU
- Emanuel Larsson LU
- opponent
-
- Prof. Först, Petra, Technical University of Munich, Germany.
- organization
- publishing date
- 2024-01-09
- type
- Thesis
- publication status
- published
- subject
- keywords
- freeze-drying, lyophilization, L.ruteri, Probiotics, Lyoprotectant, Structure, Encapsulating material, Pore size, Material thickness, SEM, Tomography, Storage stability, Amorphous sugars, Glass transition temperature, DSC
- volume
- 1
- edition
- 1
- pages
- 146 pages
- publisher
- Department of Process and Life Science Engineering, Lund University.
- defense location
- Lecture Hall KC:A, Kemicentrum, Naturvetarvägen 14, Faculty of Engineering LTH, Lund University, Lund. The dissertation will be live streamed, but part of the premises is to be excluded from the live stream.
- defense date
- 2024-02-02 09:15:00
- ISBN
- 978-91-8096-018-2
- 978-91-8096-019-9
- project
- NextBioform - Formulation for Biologics
- language
- English
- LU publication?
- yes
- id
- 273021c0-1867-48d8-88b5-967a5c120c49
- date added to LUP
- 2024-01-08 16:33:13
- date last changed
- 2024-01-15 10:27:35
@phdthesis{273021c0-1867-48d8-88b5-967a5c120c49,
abstract = {{This research dives into the intricate world of freeze-drying, an essential process in pharmaceutical and probiotic industries, to better understand the factors affecting probiotic stability during storage. The process of freeze-drying and the composition of freeze-dried products significantly affect the stability of probiotics, yet factors such as the material’s encapsulation properties remain relatively unexplored, demanding further research.<br/>This thesis will explore the effects of lyoprotectants, freezing, annealing, and drying on the structure of freeze-dried products. It aims to provide a comprehensive understanding of how varying conditions influence structural characteristics and their correlation with the storage stability of probiotics. We will also explore the key differences between vial and pellet formulations, and discuss topics critical for ensuring stability during storage such as glass transition temperatures.<br/>The investigation uses techniques such as scanning electron microscopy (SEM) and X-ray micro- computed tomography (μCT) to investigate the structural attributes of freeze-dried material. The thickness of the encapsulating material is the central focus, revealing its crucial role in protecting the probiotic cells. The findings indicates that a notably thicker material provides increased protection, enhancing probiotic storage stability.}},
author = {{Bai, Shuai}},
isbn = {{978-91-8096-018-2}},
keywords = {{freeze-drying; lyophilization; L.ruteri; Probiotics; Lyoprotectant; Structure; Encapsulating material; Pore size; Material thickness; SEM; Tomography; Storage stability; Amorphous sugars; Glass transition temperature; DSC}},
language = {{eng}},
month = {{01}},
publisher = {{Department of Process and Life Science Engineering, Lund University.}},
school = {{Lund University}},
title = {{Enhancing the stability of probiotics: Freeze-drying and encapsulation}},
url = {{https://lup.lub.lu.se/search/files/168601638/Enhancing_the_stability_of_probiotics.pdf}},
volume = {{1}},
year = {{2024}},
}