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Suppression of the external mitochondrial NADPH dehydrogenase, NDB1, in Arabidopsis thaliana affects central metabolism and vegetative growth.

Wallström, Sabá LU ; Florez-Sarasa, Igor; Araújo, Wagner L; Aidemark, Mari LU ; Fernández Fernández, María; Fernie, Alisdair R; Ribas-Carbó, Miquel and Rasmusson, Allan LU (2014) In Molecular Plant 7(2). p.356-368
Abstract
Ca(2+)-dependent oxidation of cytosolic NADPH is mediated by NDB1, which is an external type II NADPH dehydrogenase in the plant mitochondrial electron transport chain. Using RNA interference, the NDB1 transcript was suppressed by 80% in Arabidopsis thaliana plants, and external Ca(2+)-dependent NADPH dehydrogenase activity became undetectable in isolated mitochondria. This was linked to a decreased level of NADP+ in rosettes of the transgenic lines. Sterile-grown transgenic seedlings displayed decreased growth specifically on glucose, and respiratory metabolism of (14)C-glucose was increased. On soil, NDB1-suppressing plants had a decreased vegetative biomass, but leaf maximum quantum efficiency of photosystem II and CO2 assimilation... (More)
Ca(2+)-dependent oxidation of cytosolic NADPH is mediated by NDB1, which is an external type II NADPH dehydrogenase in the plant mitochondrial electron transport chain. Using RNA interference, the NDB1 transcript was suppressed by 80% in Arabidopsis thaliana plants, and external Ca(2+)-dependent NADPH dehydrogenase activity became undetectable in isolated mitochondria. This was linked to a decreased level of NADP+ in rosettes of the transgenic lines. Sterile-grown transgenic seedlings displayed decreased growth specifically on glucose, and respiratory metabolism of (14)C-glucose was increased. On soil, NDB1-suppressing plants had a decreased vegetative biomass, but leaf maximum quantum efficiency of photosystem II and CO2 assimilation rates, as well as total respiration were similar to the wild type. The in vivo alternative oxidase activity and capacity were also similar in all genotypes. Metabolic profiling revealed decreased levels of sugars, citric acid cycle intermediates and amino acids in the transgenic lines. The NDB1-suppression induced transcriptomic changes associated with protein synthesis and glucosinolate and jasmonate metabolism. The transcriptomic changes also overlapped with changes observed in a mutant lacking ABAINSENSITIVE4 and in A. thaliana overexpressing stress tolerance genes from rice. The results thus indicate that A. thaliana NDB1 modulates NADP(H) reduction levels, which in turn affect central metabolism and growth, and interact with defence signalling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
vegetative growth., type II NAD(P)H dehydrogenase, citric acid cycle, metabolic profiling, mitochondrial respiration, NADPH, RNA interference
in
Molecular Plant
volume
7
issue
2
pages
356 - 368
publisher
Oxford University Press
external identifiers
  • wos:000330841400008
  • pmid:23939432
  • scopus:84893044452
ISSN
1752-9867
DOI
10.1093/mp/sst115
language
English
LU publication?
yes
id
042d1d24-460f-49cd-9a3c-cffc490182ce (old id 4005802)
date added to LUP
2013-09-17 12:30:25
date last changed
2017-09-03 03:24:46
@article{042d1d24-460f-49cd-9a3c-cffc490182ce,
  abstract     = {Ca(2+)-dependent oxidation of cytosolic NADPH is mediated by NDB1, which is an external type II NADPH dehydrogenase in the plant mitochondrial electron transport chain. Using RNA interference, the NDB1 transcript was suppressed by 80% in Arabidopsis thaliana plants, and external Ca(2+)-dependent NADPH dehydrogenase activity became undetectable in isolated mitochondria. This was linked to a decreased level of NADP+ in rosettes of the transgenic lines. Sterile-grown transgenic seedlings displayed decreased growth specifically on glucose, and respiratory metabolism of (14)C-glucose was increased. On soil, NDB1-suppressing plants had a decreased vegetative biomass, but leaf maximum quantum efficiency of photosystem II and CO2 assimilation rates, as well as total respiration were similar to the wild type. The in vivo alternative oxidase activity and capacity were also similar in all genotypes. Metabolic profiling revealed decreased levels of sugars, citric acid cycle intermediates and amino acids in the transgenic lines. The NDB1-suppression induced transcriptomic changes associated with protein synthesis and glucosinolate and jasmonate metabolism. The transcriptomic changes also overlapped with changes observed in a mutant lacking ABAINSENSITIVE4 and in A. thaliana overexpressing stress tolerance genes from rice. The results thus indicate that A. thaliana NDB1 modulates NADP(H) reduction levels, which in turn affect central metabolism and growth, and interact with defence signalling.},
  author       = {Wallström, Sabá and Florez-Sarasa, Igor and Araújo, Wagner L and Aidemark, Mari and Fernández Fernández, María and Fernie, Alisdair R and Ribas-Carbó, Miquel and Rasmusson, Allan},
  issn         = {1752-9867},
  keyword      = {vegetative growth.,type II NAD(P)H dehydrogenase,citric acid cycle,metabolic profiling,mitochondrial respiration,NADPH,RNA interference},
  language     = {eng},
  number       = {2},
  pages        = {356--368},
  publisher    = {Oxford University Press},
  series       = {Molecular Plant},
  title        = {Suppression of the external mitochondrial NADPH dehydrogenase, NDB1, in Arabidopsis thaliana affects central metabolism and vegetative growth.},
  url          = {http://dx.doi.org/10.1093/mp/sst115},
  volume       = {7},
  year         = {2014},
}