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Laccase-based biosensors for monitoring lignin

Shleev, Sergey LU ; Persson, Per LU ; Shumakovich, Galina ; Mazhugo, Yulia ; Yaropolov, Alexander ; Ruzgas, Tautgirdas LU and Gorton, Lo LU (2006) In Enzyme and Microbial Technology 39(4). p.835-840
Abstract
Four different ways to detect Kraft and soluble pine lignin using either soluble or immobilised Trametes hirsuta laccase have been investigated. In the first, the concentration of lignin was measured using laccase in homogeneous medium with a Clark electrode. In the second, laccase immobilised onto aminated porous glass beads was used in a reactor submerged into the lignin containing solution and the reaction was followed with a Clark electrode. The third and fourth techniques involved lignin detection using laccase modified spectrographic graphite electrodes, either used in flow injection mode or in batch mode, where lignin solutions were injected into an electrochemical cell. It was shown that the use of laccase modified graphite... (More)
Four different ways to detect Kraft and soluble pine lignin using either soluble or immobilised Trametes hirsuta laccase have been investigated. In the first, the concentration of lignin was measured using laccase in homogeneous medium with a Clark electrode. In the second, laccase immobilised onto aminated porous glass beads was used in a reactor submerged into the lignin containing solution and the reaction was followed with a Clark electrode. The third and fourth techniques involved lignin detection using laccase modified spectrographic graphite electrodes, either used in flow injection mode or in batch mode, where lignin solutions were injected into an electrochemical cell. It was shown that the use of laccase modified graphite electrodes in conjunction with flow injection for amperometric lignin detection is an attractive perspective and our data form the basis for designing laccase-based biosensors to be applied for measuring lignin and its model compounds in waste water, e.g. from the pulp and paper industry. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amperometry, biosensor, lignin, laccase
in
Enzyme and Microbial Technology
volume
39
issue
4
pages
835 - 840
publisher
Elsevier
external identifiers
  • wos:000238932500048
  • scopus:33745225002
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2006.01.009
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
82d0b483-06b4-4563-a9de-1e829f4bd89b (old id 402227)
date added to LUP
2016-04-01 11:51:43
date last changed
2021-09-22 02:45:44
@article{82d0b483-06b4-4563-a9de-1e829f4bd89b,
  abstract     = {Four different ways to detect Kraft and soluble pine lignin using either soluble or immobilised Trametes hirsuta laccase have been investigated. In the first, the concentration of lignin was measured using laccase in homogeneous medium with a Clark electrode. In the second, laccase immobilised onto aminated porous glass beads was used in a reactor submerged into the lignin containing solution and the reaction was followed with a Clark electrode. The third and fourth techniques involved lignin detection using laccase modified spectrographic graphite electrodes, either used in flow injection mode or in batch mode, where lignin solutions were injected into an electrochemical cell. It was shown that the use of laccase modified graphite electrodes in conjunction with flow injection for amperometric lignin detection is an attractive perspective and our data form the basis for designing laccase-based biosensors to be applied for measuring lignin and its model compounds in waste water, e.g. from the pulp and paper industry. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Shleev, Sergey and Persson, Per and Shumakovich, Galina and Mazhugo, Yulia and Yaropolov, Alexander and Ruzgas, Tautgirdas and Gorton, Lo},
  issn         = {0141-0229},
  language     = {eng},
  number       = {4},
  pages        = {835--840},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Laccase-based biosensors for monitoring lignin},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2006.01.009},
  doi          = {10.1016/j.enzmictec.2006.01.009},
  volume       = {39},
  year         = {2006},
}