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Intraduodenal infusion of alpha-ketoglutarate decreases whole body energy expenditure in growing pigs

Junghans, Peter ; Derno, Michael ; Pierzynowski, Stefan LU ; Hennig, Ulf ; Rudolph, Paul Eberhard and Souffrant, Wolfgang B. (2006) In Clinical Nutrition 25(3). p.489-496
Abstract
Background & aims: alpha-Ketoglutarate (AKG) has been suggested to play a particular role as an oxidative fuel for the gut, and thus may have a sparing function for fuels such as glutamate and aspartate. Using the pig model we aimed to quantify how the route of administration (intravenous, i.v.; intragastric, i.g.; intraduodenal, i.d.) affects AKG utilization, whole body energy expenditure (EE) and nutrient oxidation. Methods: Pigs (15 kg) were supplied with a complete nutrient solution (NS) via catheters. To explore the metabolic effects of AKG, 1.0 g AKG kgBW(-1) d(-1) was infused simultaneously with the NS using either the i.d., i.v. or i.g. route. [1-C-13]AKG (15 mg kgBW(-1)) was infused i.d., i.v. or i.g., respectively, for 3 h.... (More)
Background & aims: alpha-Ketoglutarate (AKG) has been suggested to play a particular role as an oxidative fuel for the gut, and thus may have a sparing function for fuels such as glutamate and aspartate. Using the pig model we aimed to quantify how the route of administration (intravenous, i.v.; intragastric, i.g.; intraduodenal, i.d.) affects AKG utilization, whole body energy expenditure (EE) and nutrient oxidation. Methods: Pigs (15 kg) were supplied with a complete nutrient solution (NS) via catheters. To explore the metabolic effects of AKG, 1.0 g AKG kgBW(-1) d(-1) was infused simultaneously with the NS using either the i.d., i.v. or i.g. route. [1-C-13]AKG (15 mg kgBW(-1)) was infused i.d., i.v. or i.g., respectively, for 3 h. AKG utilization (AKG UTIL) was estimated as AKG UTIL = 100-C-13 recovery (% of C-13 dose). C-13 recovery was calculated from the C-13 enrichment in breath CO2 and the whole-body CO2 production. Results: AKG infusion and NS via the i.d. route resulted in a reduced AKG UTIL (40.1 +/- 6.7) as compared to the i.v. route (62.9 +/- 2.4, P < 0.001) and i.g. route (62.3 +/- 1.6, P < 0.001). The total EE was lower with the i.d. route of AKG and NS (745 +/- 68 kJ kgBW(-0.62) d(-1)) as compared to the i.v. route (965 +/- 54 kJ kgBW(-0.62) d(-1), P < 0.005) and i.g. route (918 +/- 43 kJ kgBW(-0.62) d(-1), P < 0.005). Carbohydrate oxidation was increased with the i.d. route (38.2g +/- 3.4 kg BW-0.62 d(-1)) as compared to the i.v. route (27.8 +/- 2.9g kg BW-0.62 d(-1), P < 0.08) and i.g. route (23.9 +/- 8.5g kg BW-0.62 d(-1), P < 0.05). Fat oxidation was decreased (2.1 +/- 1.9 g kgBW(-0.62) d(-1); P < 0.001) with the i.d. route as compared to the i.v. route (11.5 coproduct 1.4g kgBW(-0.62) d(-1), P < 0.001) and i.g. route (11.9 +/- 3.1 g kgBW(-0.62) d(-1), P < 0.001). Conclusions: The i.d. infusion of AKG in combination with the NS affected the whole body EE and nutrient oxidation, in comparison to that obtained with the i.v. and i.g. routes. It was concluded that the i.d. administration of AKG markedly controlled the nutrient partitioning in the oxidation processes. Finally, in contrary to the observations with glutamine or glutamate, a considerable percentage of the AKG infusion was retained in the body irrespective of the route of administration. (C) 2005 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
energy expenditure, alpha-ketoglutarate, administration route, pig model
in
Clinical Nutrition
volume
25
issue
3
pages
489 - 496
publisher
Elsevier
external identifiers
  • wos:000239127000015
  • scopus:33744935791
ISSN
1532-1983
DOI
10.1016/j.clnu.2005.11.003
language
English
LU publication?
yes
id
d81bdb1a-c71d-4345-a503-7f18558bc733 (old id 402275)
date added to LUP
2016-04-01 16:08:34
date last changed
2020-08-05 03:23:10
@article{d81bdb1a-c71d-4345-a503-7f18558bc733,
  abstract     = {Background &amp; aims: alpha-Ketoglutarate (AKG) has been suggested to play a particular role as an oxidative fuel for the gut, and thus may have a sparing function for fuels such as glutamate and aspartate. Using the pig model we aimed to quantify how the route of administration (intravenous, i.v.; intragastric, i.g.; intraduodenal, i.d.) affects AKG utilization, whole body energy expenditure (EE) and nutrient oxidation. Methods: Pigs (15 kg) were supplied with a complete nutrient solution (NS) via catheters. To explore the metabolic effects of AKG, 1.0 g AKG kgBW(-1) d(-1) was infused simultaneously with the NS using either the i.d., i.v. or i.g. route. [1-C-13]AKG (15 mg kgBW(-1)) was infused i.d., i.v. or i.g., respectively, for 3 h. AKG utilization (AKG UTIL) was estimated as AKG UTIL = 100-C-13 recovery (% of C-13 dose). C-13 recovery was calculated from the C-13 enrichment in breath CO2 and the whole-body CO2 production. Results: AKG infusion and NS via the i.d. route resulted in a reduced AKG UTIL (40.1 +/- 6.7) as compared to the i.v. route (62.9 +/- 2.4, P &lt; 0.001) and i.g. route (62.3 +/- 1.6, P &lt; 0.001). The total EE was lower with the i.d. route of AKG and NS (745 +/- 68 kJ kgBW(-0.62) d(-1)) as compared to the i.v. route (965 +/- 54 kJ kgBW(-0.62) d(-1), P &lt; 0.005) and i.g. route (918 +/- 43 kJ kgBW(-0.62) d(-1), P &lt; 0.005). Carbohydrate oxidation was increased with the i.d. route (38.2g +/- 3.4 kg BW-0.62 d(-1)) as compared to the i.v. route (27.8 +/- 2.9g kg BW-0.62 d(-1), P &lt; 0.08) and i.g. route (23.9 +/- 8.5g kg BW-0.62 d(-1), P &lt; 0.05). Fat oxidation was decreased (2.1 +/- 1.9 g kgBW(-0.62) d(-1); P &lt; 0.001) with the i.d. route as compared to the i.v. route (11.5 coproduct 1.4g kgBW(-0.62) d(-1), P &lt; 0.001) and i.g. route (11.9 +/- 3.1 g kgBW(-0.62) d(-1), P &lt; 0.001). Conclusions: The i.d. infusion of AKG in combination with the NS affected the whole body EE and nutrient oxidation, in comparison to that obtained with the i.v. and i.g. routes. It was concluded that the i.d. administration of AKG markedly controlled the nutrient partitioning in the oxidation processes. Finally, in contrary to the observations with glutamine or glutamate, a considerable percentage of the AKG infusion was retained in the body irrespective of the route of administration. (C) 2005 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.},
  author       = {Junghans, Peter and Derno, Michael and Pierzynowski, Stefan and Hennig, Ulf and Rudolph, Paul Eberhard and Souffrant, Wolfgang B.},
  issn         = {1532-1983},
  language     = {eng},
  number       = {3},
  pages        = {489--496},
  publisher    = {Elsevier},
  series       = {Clinical Nutrition},
  title        = {Intraduodenal infusion of alpha-ketoglutarate decreases whole body energy expenditure in growing pigs},
  url          = {http://dx.doi.org/10.1016/j.clnu.2005.11.003},
  doi          = {10.1016/j.clnu.2005.11.003},
  volume       = {25},
  year         = {2006},
}