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Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains

Karhumaa, Kaisa LU ; Wiedemann, B ; Hahn-Hägerdal, Bärbel LU ; Boles, E and Gorwa-Grauslund, Marie-Francoise LU (2006) In Microbial Cell Factories 5.
Abstract
Background: Fermentation of lignocellulosic biomass is an attractive alternative for the production of bioethanol. Traditionally, the yeast Saccharomyces cerevisiae is used in industrial ethanol fermentations. However, S. cerevisiae is naturally not able to ferment the pentose sugars D-xylose and L-arabinose, which are present in high amounts in lignocellulosic raw materials. Results: We describe the engineering of laboratory and industrial S. cerevisiae strains to co-ferment the pentose sugars D-xylose and L-arabinose. Introduction of a fungal xylose and a bacterial arabinose pathway resulted in strains able to grow on both pentose sugars. Introduction of a xylose pathway into an arabinose-fermenting laboratory strain resulted in nearly... (More)
Background: Fermentation of lignocellulosic biomass is an attractive alternative for the production of bioethanol. Traditionally, the yeast Saccharomyces cerevisiae is used in industrial ethanol fermentations. However, S. cerevisiae is naturally not able to ferment the pentose sugars D-xylose and L-arabinose, which are present in high amounts in lignocellulosic raw materials. Results: We describe the engineering of laboratory and industrial S. cerevisiae strains to co-ferment the pentose sugars D-xylose and L-arabinose. Introduction of a fungal xylose and a bacterial arabinose pathway resulted in strains able to grow on both pentose sugars. Introduction of a xylose pathway into an arabinose-fermenting laboratory strain resulted in nearly complete conversion of arabinose into arabitol due to the L-arabinose reductase activity of the xylose reductase. The industrial strain displayed lower arabitol yield and increased ethanol yield from xylose and arabinose. Conclusion: Our work demonstrates simultaneous co-utilization of xylose and arabinose in recombinant strains of S. cerevisiae. In addition, the co-utilization of arabinose together with xylose significantly reduced formation of the by-product xylitol, which contributed to improved ethanol production. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbial Cell Factories
volume
5
publisher
BioMed Central (BMC)
external identifiers
  • wos:000237573300001
  • pmid:16606456
  • scopus:33646569083
ISSN
1475-2859
DOI
10.1186/1475-2859-5-18
language
English
LU publication?
yes
id
028e7df4-5030-4631-a8b3-3d9efef2a02c (old id 409445)
date added to LUP
2016-04-01 16:23:22
date last changed
2021-09-22 02:08:39
@article{028e7df4-5030-4631-a8b3-3d9efef2a02c,
  abstract     = {Background: Fermentation of lignocellulosic biomass is an attractive alternative for the production of bioethanol. Traditionally, the yeast Saccharomyces cerevisiae is used in industrial ethanol fermentations. However, S. cerevisiae is naturally not able to ferment the pentose sugars D-xylose and L-arabinose, which are present in high amounts in lignocellulosic raw materials. Results: We describe the engineering of laboratory and industrial S. cerevisiae strains to co-ferment the pentose sugars D-xylose and L-arabinose. Introduction of a fungal xylose and a bacterial arabinose pathway resulted in strains able to grow on both pentose sugars. Introduction of a xylose pathway into an arabinose-fermenting laboratory strain resulted in nearly complete conversion of arabinose into arabitol due to the L-arabinose reductase activity of the xylose reductase. The industrial strain displayed lower arabitol yield and increased ethanol yield from xylose and arabinose. Conclusion: Our work demonstrates simultaneous co-utilization of xylose and arabinose in recombinant strains of S. cerevisiae. In addition, the co-utilization of arabinose together with xylose significantly reduced formation of the by-product xylitol, which contributed to improved ethanol production.},
  author       = {Karhumaa, Kaisa and Wiedemann, B and Hahn-Hägerdal, Bärbel and Boles, E and Gorwa-Grauslund, Marie-Francoise},
  issn         = {1475-2859},
  language     = {eng},
  publisher    = {BioMed Central (BMC)},
  series       = {Microbial Cell Factories},
  title        = {Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains},
  url          = {http://dx.doi.org/10.1186/1475-2859-5-18},
  doi          = {10.1186/1475-2859-5-18},
  volume       = {5},
  year         = {2006},
}